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Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma.

Wu G, Wang Y, Lu X, He H, Liu H, Meng X, Xia S, Zheng K, Liu B - BMC Cancer (2015)

Bottom Line: However, results have been conflicting regarding its expression levels and role in HCC.Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression. miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis.Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. wgzwl@hotmail.com.

ABSTRACT

Background: Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC.

Methods: RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP). Transfection of miR-372 mimic into HCC cell lines was used to evaluate cellular proliferation and invasion. Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression.

Results: miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis. Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment. Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells.

Conclusions: miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

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Related in: MedlinePlus

RT-PCR tested Mir-372 expression in 37 HCC tissues samples and corresponding normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01). a: ΔCt (U6B minus mir-372) was used to compared the expression difference between tumor and normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01); b: CpG islands located approximately 1,200 bp upstream of the promoter.
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Fig1: RT-PCR tested Mir-372 expression in 37 HCC tissues samples and corresponding normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01). a: ΔCt (U6B minus mir-372) was used to compared the expression difference between tumor and normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01); b: CpG islands located approximately 1,200 bp upstream of the promoter.

Mentions: RT-PCR showed that the mean expression levels of miR-372 were lower in HCC tissues compared with normal tissues (−14.89 ± 2.83 vs. −12.38 ± 2.96, respectively; P <0.01) (Figure 1). Of 120 HCC patients, miR-372 was expressed at low levels in 87 cases (72.5%) according to in situ hybridization (Figure 2). Furthermore, HUH7 and HCCLM3 HCC cell lines showed lower expression levels of miR-372 than HepG2, SMMC7721, PLC5, QGY7701 and LO2 cells (Figure 3a).Figure 1


Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma.

Wu G, Wang Y, Lu X, He H, Liu H, Meng X, Xia S, Zheng K, Liu B - BMC Cancer (2015)

RT-PCR tested Mir-372 expression in 37 HCC tissues samples and corresponding normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01). a: ΔCt (U6B minus mir-372) was used to compared the expression difference between tumor and normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01); b: CpG islands located approximately 1,200 bp upstream of the promoter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4379970&req=5

Fig1: RT-PCR tested Mir-372 expression in 37 HCC tissues samples and corresponding normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01). a: ΔCt (U6B minus mir-372) was used to compared the expression difference between tumor and normal tissues (−14.89 ± 2.83 vs −12.38 ± 2.96, **P < 0.01); b: CpG islands located approximately 1,200 bp upstream of the promoter.
Mentions: RT-PCR showed that the mean expression levels of miR-372 were lower in HCC tissues compared with normal tissues (−14.89 ± 2.83 vs. −12.38 ± 2.96, respectively; P <0.01) (Figure 1). Of 120 HCC patients, miR-372 was expressed at low levels in 87 cases (72.5%) according to in situ hybridization (Figure 2). Furthermore, HUH7 and HCCLM3 HCC cell lines showed lower expression levels of miR-372 than HepG2, SMMC7721, PLC5, QGY7701 and LO2 cells (Figure 3a).Figure 1

Bottom Line: However, results have been conflicting regarding its expression levels and role in HCC.Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression. miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis.Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. wgzwl@hotmail.com.

ABSTRACT

Background: Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC.

Methods: RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP). Transfection of miR-372 mimic into HCC cell lines was used to evaluate cellular proliferation and invasion. Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression.

Results: miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis. Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment. Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells.

Conclusions: miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

Show MeSH
Related in: MedlinePlus