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Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

Lavinskiene S, Malakauskas K, Jeroch J, Hoppenot D, Sakalauskas R - J Inflamm (Lond) (2015)

Bottom Line: Before bronchial allergen challenge, peripheral blood eosinophil chemotaxis, spontaneous ROS production was enhanced and eosinophil apoptosis was reduced in the patients with AA as compared with AR patients and HS (P < 0.05).Meanwhile, eosinophil chemotaxis and ROS generation markedly increased in the patients with AA 7 h and 24 h after challenge compared with other groups and baseline values (P < 0.05).The percentage of apoptotic eosinophils in the patients with AA decreased at 7 h as well as 24 h after challenge when compared with other groups and the baseline values (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonology and Immunology, Lithuanian University of Health Sciences, Kaunas, Lithuanian.

ABSTRACT

Objective: We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.

Material and methods: A total of 18 patients with allergic asthma (AA), 14 with allergic rhinitis (AR), and 10 healthy subjects (HS) underwent bronchial challenge with a specific allergen extract. Eosinophils from peripheral blood were isolated 24 h before as well as 7 and 24 h after bronchial allergen challenge. Chemotaxis, spontaneous ROS production in eosinophils, and apoptosis were analyzed by flow cytometry. Serum and induced sputum IL-5 levels were measured by ELISA; the cell count in sputum was analyzed by the May-Grünwald-Giemsa method.

Results: Before bronchial allergen challenge, peripheral blood eosinophil chemotaxis, spontaneous ROS production was enhanced and eosinophil apoptosis was reduced in the patients with AA as compared with AR patients and HS (P < 0.05). Meanwhile, eosinophil chemotaxis and ROS generation markedly increased in the patients with AA 7 h and 24 h after challenge compared with other groups and baseline values (P < 0.05). The percentage of apoptotic eosinophils in the patients with AA decreased at 7 h as well as 24 h after challenge when compared with other groups and the baseline values (P < 0.05). There was a significant correlation between the migrated peripheral blood eosinophil count and the sputum eosinophil count (Rs = 0.89, P < 0.0001) and the sputum IL-5 level (Rs = 0.68, P = 0.002) at 24 h after bronchial challenge only in the patients with AA. Furthermore, the percentage of peripheral blood apoptotic eosinophils significantly correlated with eosinophil count in sputum (Rs = -0.53, P = 0.02), and ROS production correlated with the serum IL-5 levels (Rs = 0.71, P = 0.01).

Conclusion: During allergen-induced late-phase airway inflammation, peripheral blood eosinophils demonstrated further alterations of their functional activity manifested by enhanced spontaneous ROS production, increased chemotaxis, and diminished apoptosis in patients with AA.

No MeSH data available.


Related in: MedlinePlus

Correlations between peripheral blood eosinophil activity and eosinophil count as well as IL-5 levels in the induced sputum in the patients with allergic asthma 24 h after bronchial allergen challenge.A Correlation between migrated peripheral blood eosinophil count and eosinophil count in sputum. B Correlation between percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum. C Correlation between migrated peripheral blood eosinophil count and IL-5 level in sputum. D Correlation between ROS production in peripheral blood eosinophils and serum IL-5 levels. MFI indicates mean fluorescence intensity.
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Fig6: Correlations between peripheral blood eosinophil activity and eosinophil count as well as IL-5 levels in the induced sputum in the patients with allergic asthma 24 h after bronchial allergen challenge.A Correlation between migrated peripheral blood eosinophil count and eosinophil count in sputum. B Correlation between percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum. C Correlation between migrated peripheral blood eosinophil count and IL-5 level in sputum. D Correlation between ROS production in peripheral blood eosinophils and serum IL-5 levels. MFI indicates mean fluorescence intensity.

Mentions: Significant correlations were found only in the patients with allergic asthma at 24 h after bronchial allergen challenge. The migrated peripheral blood eosinophil count significantly correlated with the eosinophil count in the sputum (Rs = 0.89, P < 0.0001; Figure 6A). Moreover, there was a significant correlation between the percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum (Rs = −0.53, P =0.02; Figure 6B). The migrated peripheral blood eosinophil count significantly correlated with sputum IL-5 levels (Rs = 0.68, P = 0.002; Figure 6C), and ROS production in peripheral blood eosinophils significantly correlated with the serum IL-5 levels (Rs = 0.71, P = 0.01; Figure 6D).Figure 6


Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

Lavinskiene S, Malakauskas K, Jeroch J, Hoppenot D, Sakalauskas R - J Inflamm (Lond) (2015)

Correlations between peripheral blood eosinophil activity and eosinophil count as well as IL-5 levels in the induced sputum in the patients with allergic asthma 24 h after bronchial allergen challenge.A Correlation between migrated peripheral blood eosinophil count and eosinophil count in sputum. B Correlation between percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum. C Correlation between migrated peripheral blood eosinophil count and IL-5 level in sputum. D Correlation between ROS production in peripheral blood eosinophils and serum IL-5 levels. MFI indicates mean fluorescence intensity.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4379944&req=5

Fig6: Correlations between peripheral blood eosinophil activity and eosinophil count as well as IL-5 levels in the induced sputum in the patients with allergic asthma 24 h after bronchial allergen challenge.A Correlation between migrated peripheral blood eosinophil count and eosinophil count in sputum. B Correlation between percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum. C Correlation between migrated peripheral blood eosinophil count and IL-5 level in sputum. D Correlation between ROS production in peripheral blood eosinophils and serum IL-5 levels. MFI indicates mean fluorescence intensity.
Mentions: Significant correlations were found only in the patients with allergic asthma at 24 h after bronchial allergen challenge. The migrated peripheral blood eosinophil count significantly correlated with the eosinophil count in the sputum (Rs = 0.89, P < 0.0001; Figure 6A). Moreover, there was a significant correlation between the percentage of apoptotic peripheral blood eosinophils and eosinophil count in sputum (Rs = −0.53, P =0.02; Figure 6B). The migrated peripheral blood eosinophil count significantly correlated with sputum IL-5 levels (Rs = 0.68, P = 0.002; Figure 6C), and ROS production in peripheral blood eosinophils significantly correlated with the serum IL-5 levels (Rs = 0.71, P = 0.01; Figure 6D).Figure 6

Bottom Line: Before bronchial allergen challenge, peripheral blood eosinophil chemotaxis, spontaneous ROS production was enhanced and eosinophil apoptosis was reduced in the patients with AA as compared with AR patients and HS (P < 0.05).Meanwhile, eosinophil chemotaxis and ROS generation markedly increased in the patients with AA 7 h and 24 h after challenge compared with other groups and baseline values (P < 0.05).The percentage of apoptotic eosinophils in the patients with AA decreased at 7 h as well as 24 h after challenge when compared with other groups and the baseline values (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonology and Immunology, Lithuanian University of Health Sciences, Kaunas, Lithuanian.

ABSTRACT

Objective: We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.

Material and methods: A total of 18 patients with allergic asthma (AA), 14 with allergic rhinitis (AR), and 10 healthy subjects (HS) underwent bronchial challenge with a specific allergen extract. Eosinophils from peripheral blood were isolated 24 h before as well as 7 and 24 h after bronchial allergen challenge. Chemotaxis, spontaneous ROS production in eosinophils, and apoptosis were analyzed by flow cytometry. Serum and induced sputum IL-5 levels were measured by ELISA; the cell count in sputum was analyzed by the May-Grünwald-Giemsa method.

Results: Before bronchial allergen challenge, peripheral blood eosinophil chemotaxis, spontaneous ROS production was enhanced and eosinophil apoptosis was reduced in the patients with AA as compared with AR patients and HS (P < 0.05). Meanwhile, eosinophil chemotaxis and ROS generation markedly increased in the patients with AA 7 h and 24 h after challenge compared with other groups and baseline values (P < 0.05). The percentage of apoptotic eosinophils in the patients with AA decreased at 7 h as well as 24 h after challenge when compared with other groups and the baseline values (P < 0.05). There was a significant correlation between the migrated peripheral blood eosinophil count and the sputum eosinophil count (Rs = 0.89, P < 0.0001) and the sputum IL-5 level (Rs = 0.68, P = 0.002) at 24 h after bronchial challenge only in the patients with AA. Furthermore, the percentage of peripheral blood apoptotic eosinophils significantly correlated with eosinophil count in sputum (Rs = -0.53, P = 0.02), and ROS production correlated with the serum IL-5 levels (Rs = 0.71, P = 0.01).

Conclusion: During allergen-induced late-phase airway inflammation, peripheral blood eosinophils demonstrated further alterations of their functional activity manifested by enhanced spontaneous ROS production, increased chemotaxis, and diminished apoptosis in patients with AA.

No MeSH data available.


Related in: MedlinePlus