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Analysis of microbial communities in the oil reservoir subjected to CO2-flooding by using functional genes as molecular biomarkers for microbial CO2 sequestration.

Liu JF, Sun XB, Yang GC, Mbadinga SM, Gu JD, Mu BZ - Front Microbiol (2015)

Bottom Line: As a comparison, these functional genes in the production water samples from oil reservoir only experienced water-flooding in areas of the same oil bearing bed were also analyzed.It showed that these functional genes were all of rich diversity in these samples, and the functional microbial communities and their diversity were strongly affected by a long-term exposure to injected CO2.More interestingly, microorganisms affiliated with members of the genera Methanothemobacter, Acetobacterium, and Halothiobacillus as well as hydrogen producers in CO2 injected area either increased or remained unchanged in relative abundance compared to that in water-flooded area, which implied that these microorganisms could adapt to CO2 injection and, if so, demonstrated the potential for microbial fixation and conversion of CO2 into methane in subsurface oil reservoirs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology Shanghai, China.

ABSTRACT
Sequestration of CO2 in oil reservoirs is considered to be one of the feasible options for mitigating atmospheric CO2 building up and also for the in situ potential bioconversion of stored CO2 to methane. However, the information on these functional microbial communities and the impact of CO2 storage on them is hardly available. In this paper a comprehensive molecular survey was performed on microbial communities in production water samples from oil reservoirs experienced CO2-flooding by analysis of functional genes involved in the process, including cbbM, cbbL, fthfs, [FeFe]-hydrogenase, and mcrA. As a comparison, these functional genes in the production water samples from oil reservoir only experienced water-flooding in areas of the same oil bearing bed were also analyzed. It showed that these functional genes were all of rich diversity in these samples, and the functional microbial communities and their diversity were strongly affected by a long-term exposure to injected CO2. More interestingly, microorganisms affiliated with members of the genera Methanothemobacter, Acetobacterium, and Halothiobacillus as well as hydrogen producers in CO2 injected area either increased or remained unchanged in relative abundance compared to that in water-flooded area, which implied that these microorganisms could adapt to CO2 injection and, if so, demonstrated the potential for microbial fixation and conversion of CO2 into methane in subsurface oil reservoirs.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of the [FeFe]-Hydrogenase gene retrieved from the water samples (colored) and closely related sequences from GenBank database. Alignments to related sequences (shown with accession number) were performed with MEGA 5 software. The topology of the tree was obtained with the neighbor-joining method. Bootstrap values (n = 1000 replicates) greater than 50% are reported. Scale bar represents 10% amino acid substitution.
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Figure 5: Phylogenetic tree of the [FeFe]-Hydrogenase gene retrieved from the water samples (colored) and closely related sequences from GenBank database. Alignments to related sequences (shown with accession number) were performed with MEGA 5 software. The topology of the tree was obtained with the neighbor-joining method. Bootstrap values (n = 1000 replicates) greater than 50% are reported. Scale bar represents 10% amino acid substitution.

Mentions: The [FeFe]-hydrogenase-encoding gene was detected in both C and W samples, and phylogenetic analysis of the sequenced clones were assembled into 9 and 14 OTUs, respectively (Figure 5). The majority of the gene sequences obtained from the two samples cluster with sequences related to Firmicutes. One OTU represented by FeFe-Hyd_W3-6 shares similarity with Syntrophothermus lipocalidus of the Firmicutes. FeFe-Hyd_W4-38 is either related to Shewanella halifaxensis HAW-EB4 within the Gamma-Proteobacteria or to Thermodesulfovibrio yellowstonii within the Nitrospira (Figure 5). FeFe-Hyd_W4-36 is related to Thermodesulfobium narugense belonging to the family Thermodesulfobiaceae within the Firmicutes. FeFe-Hyd_W4-22 shares high identity with Moorella/ thermoacetica affiliated to the family Thermoanaerobacteraceae of Firmicutes. FeFe-Hyd_W4-4, FeFe-Hyd_C2-26, and FeFe-Hyd_W4-32 are all related to Desulfotomaculum kuznetsovii, a member of the order Clostridiales within Firmicutes. FeFe-Hyd_C2-10 and FeFe-Hyd_W4-35 are both similar to Thermotoga lettingae TMO affiliated with the family Thermotogaceae.


Analysis of microbial communities in the oil reservoir subjected to CO2-flooding by using functional genes as molecular biomarkers for microbial CO2 sequestration.

Liu JF, Sun XB, Yang GC, Mbadinga SM, Gu JD, Mu BZ - Front Microbiol (2015)

Phylogenetic tree of the [FeFe]-Hydrogenase gene retrieved from the water samples (colored) and closely related sequences from GenBank database. Alignments to related sequences (shown with accession number) were performed with MEGA 5 software. The topology of the tree was obtained with the neighbor-joining method. Bootstrap values (n = 1000 replicates) greater than 50% are reported. Scale bar represents 10% amino acid substitution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379918&req=5

Figure 5: Phylogenetic tree of the [FeFe]-Hydrogenase gene retrieved from the water samples (colored) and closely related sequences from GenBank database. Alignments to related sequences (shown with accession number) were performed with MEGA 5 software. The topology of the tree was obtained with the neighbor-joining method. Bootstrap values (n = 1000 replicates) greater than 50% are reported. Scale bar represents 10% amino acid substitution.
Mentions: The [FeFe]-hydrogenase-encoding gene was detected in both C and W samples, and phylogenetic analysis of the sequenced clones were assembled into 9 and 14 OTUs, respectively (Figure 5). The majority of the gene sequences obtained from the two samples cluster with sequences related to Firmicutes. One OTU represented by FeFe-Hyd_W3-6 shares similarity with Syntrophothermus lipocalidus of the Firmicutes. FeFe-Hyd_W4-38 is either related to Shewanella halifaxensis HAW-EB4 within the Gamma-Proteobacteria or to Thermodesulfovibrio yellowstonii within the Nitrospira (Figure 5). FeFe-Hyd_W4-36 is related to Thermodesulfobium narugense belonging to the family Thermodesulfobiaceae within the Firmicutes. FeFe-Hyd_W4-22 shares high identity with Moorella/ thermoacetica affiliated to the family Thermoanaerobacteraceae of Firmicutes. FeFe-Hyd_W4-4, FeFe-Hyd_C2-26, and FeFe-Hyd_W4-32 are all related to Desulfotomaculum kuznetsovii, a member of the order Clostridiales within Firmicutes. FeFe-Hyd_C2-10 and FeFe-Hyd_W4-35 are both similar to Thermotoga lettingae TMO affiliated with the family Thermotogaceae.

Bottom Line: As a comparison, these functional genes in the production water samples from oil reservoir only experienced water-flooding in areas of the same oil bearing bed were also analyzed.It showed that these functional genes were all of rich diversity in these samples, and the functional microbial communities and their diversity were strongly affected by a long-term exposure to injected CO2.More interestingly, microorganisms affiliated with members of the genera Methanothemobacter, Acetobacterium, and Halothiobacillus as well as hydrogen producers in CO2 injected area either increased or remained unchanged in relative abundance compared to that in water-flooded area, which implied that these microorganisms could adapt to CO2 injection and, if so, demonstrated the potential for microbial fixation and conversion of CO2 into methane in subsurface oil reservoirs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology Shanghai, China.

ABSTRACT
Sequestration of CO2 in oil reservoirs is considered to be one of the feasible options for mitigating atmospheric CO2 building up and also for the in situ potential bioconversion of stored CO2 to methane. However, the information on these functional microbial communities and the impact of CO2 storage on them is hardly available. In this paper a comprehensive molecular survey was performed on microbial communities in production water samples from oil reservoirs experienced CO2-flooding by analysis of functional genes involved in the process, including cbbM, cbbL, fthfs, [FeFe]-hydrogenase, and mcrA. As a comparison, these functional genes in the production water samples from oil reservoir only experienced water-flooding in areas of the same oil bearing bed were also analyzed. It showed that these functional genes were all of rich diversity in these samples, and the functional microbial communities and their diversity were strongly affected by a long-term exposure to injected CO2. More interestingly, microorganisms affiliated with members of the genera Methanothemobacter, Acetobacterium, and Halothiobacillus as well as hydrogen producers in CO2 injected area either increased or remained unchanged in relative abundance compared to that in water-flooded area, which implied that these microorganisms could adapt to CO2 injection and, if so, demonstrated the potential for microbial fixation and conversion of CO2 into methane in subsurface oil reservoirs.

No MeSH data available.


Related in: MedlinePlus