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Enhancement of human skin fibroblasts proliferation as a result of treating with quince seed mucilage.

Ghafourian M, Tamri P, Hemmati A - Jundishapur J Nat Pharm Prod (2015)

Bottom Line: In this study, we investigated the effects of QSM at concentrations of 50, 100, 200, and 400 µg/mL on human skin fibroblast proliferation as an aspect of promotion of wound healing.The concentration of 50 µg/mL was the most effective dose after 72 hours treatment.This effect suggests that this compound can act as a wound healing agent.

View Article: PubMed Central - PubMed

Affiliation: Physiology Research Center, Department of Immunology, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Quince seed mucilage (QSM) has been used in Iranian folk medicine in the treatment of wounds and burns. Experimental and clinical studies showed its wound healing activity. However, the mechanism by which this agent affects cells involved in the wound healing process is unknown.

Objectives: In this study, we investigated the effects of QSM at concentrations of 50, 100, 200, and 400 µg/mL on human skin fibroblast proliferation as an aspect of promotion of wound healing.

Materials and methods: Human skin fibroblast cell line (HNFF-P18) was used in the experiment. Cell proliferation assay was measured by a MTT assay.

Results: Cells treated with QSM at concentrations less than 400 µg/mL increased their proliferative activity. The concentration of 50 µg/mL was the most effective dose after 72 hours treatment.

Conclusions: QSM has the ability to stimulate proliferation of human skin fibroblast. This effect suggests that this compound can act as a wound healing agent.

No MeSH data available.


Related in: MedlinePlus

Comparison of Microscopic Aspects of Human Skin Fibroblasts Submitted to Different Concentrations of QSM After 72 Hours of IncubationA) Control group. B) QSM 50 µg/mL treated cells. C) QSM 100 µg/mL treated cells. D) QSM 200 µg/mL treated cells. E) QSM 400 µg/mL treated cells.
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fig18016: Comparison of Microscopic Aspects of Human Skin Fibroblasts Submitted to Different Concentrations of QSM After 72 Hours of IncubationA) Control group. B) QSM 50 µg/mL treated cells. C) QSM 100 µg/mL treated cells. D) QSM 200 µg/mL treated cells. E) QSM 400 µg/mL treated cells.

Mentions: The QSM cream enhanced human fibroblasts proliferation (Figure 1). This effect of QSM in concentration of 100 and 200 µg/mL was visible by MTT assay after 48 hours of treatment (P < 0.05) (Figure 2). QSM at concentration of 50 µg/mL significantly enhanced the proliferation of fibroblast compared to the untreated group after 72 hours (P < 0.01) (Figure 3). The concentration of 400 µg/mL was ineffective.


Enhancement of human skin fibroblasts proliferation as a result of treating with quince seed mucilage.

Ghafourian M, Tamri P, Hemmati A - Jundishapur J Nat Pharm Prod (2015)

Comparison of Microscopic Aspects of Human Skin Fibroblasts Submitted to Different Concentrations of QSM After 72 Hours of IncubationA) Control group. B) QSM 50 µg/mL treated cells. C) QSM 100 µg/mL treated cells. D) QSM 200 µg/mL treated cells. E) QSM 400 µg/mL treated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379889&req=5

fig18016: Comparison of Microscopic Aspects of Human Skin Fibroblasts Submitted to Different Concentrations of QSM After 72 Hours of IncubationA) Control group. B) QSM 50 µg/mL treated cells. C) QSM 100 µg/mL treated cells. D) QSM 200 µg/mL treated cells. E) QSM 400 µg/mL treated cells.
Mentions: The QSM cream enhanced human fibroblasts proliferation (Figure 1). This effect of QSM in concentration of 100 and 200 µg/mL was visible by MTT assay after 48 hours of treatment (P < 0.05) (Figure 2). QSM at concentration of 50 µg/mL significantly enhanced the proliferation of fibroblast compared to the untreated group after 72 hours (P < 0.01) (Figure 3). The concentration of 400 µg/mL was ineffective.

Bottom Line: In this study, we investigated the effects of QSM at concentrations of 50, 100, 200, and 400 µg/mL on human skin fibroblast proliferation as an aspect of promotion of wound healing.The concentration of 50 µg/mL was the most effective dose after 72 hours treatment.This effect suggests that this compound can act as a wound healing agent.

View Article: PubMed Central - PubMed

Affiliation: Physiology Research Center, Department of Immunology, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Quince seed mucilage (QSM) has been used in Iranian folk medicine in the treatment of wounds and burns. Experimental and clinical studies showed its wound healing activity. However, the mechanism by which this agent affects cells involved in the wound healing process is unknown.

Objectives: In this study, we investigated the effects of QSM at concentrations of 50, 100, 200, and 400 µg/mL on human skin fibroblast proliferation as an aspect of promotion of wound healing.

Materials and methods: Human skin fibroblast cell line (HNFF-P18) was used in the experiment. Cell proliferation assay was measured by a MTT assay.

Results: Cells treated with QSM at concentrations less than 400 µg/mL increased their proliferative activity. The concentration of 50 µg/mL was the most effective dose after 72 hours treatment.

Conclusions: QSM has the ability to stimulate proliferation of human skin fibroblast. This effect suggests that this compound can act as a wound healing agent.

No MeSH data available.


Related in: MedlinePlus