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Identification of Rubisco rbcL and rbcS in Camellia oleifera and their potential as molecular markers for selection of high tea oil cultivars.

Chen Y, Wang B, Chen J, Wang X, Wang R, Peng S, Chen L, Ma L, Luo J - Front Plant Sci (2015)

Bottom Line: The Co-rbcL expression in 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was greater than 'Hengchong 89'.The expression levels of Co-rbcS in 'Xianglin 1' and 'Xianglin 14' were similar but were significantly greater than in 'Hengchong 89'.In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Technology Research Center of Oil-tea Camellia, Hunan Academy of Forestry Changsha, China.

ABSTRACT
Tea oil derived from seeds of Camellia oleifera Abel. is high-quality edible oil in China. This study isolated full-length cDNAs of Rubisco subunits rbcL and rbcS from C. oleifera. The rbcL has 1,522 bp with a 1,425 bp coding region, encoding 475 amino acids; and the rbcS has 615 bp containing a 528 bp coding region, encoding 176 amino acids. The expression level of the two genes, designated as Co-rbcL and Co-rbcS, was determined in three C. oleifera cultivars: Hengchong 89, Xianglin 1, and Xianglin 14 whose annual oil yields were 546.9, 591.4, and 657.7 kg ha(-1), respectively. The Co-rbcL expression in 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was greater than 'Hengchong 89'. The expression levels of Co-rbcS in 'Xianglin 1' and 'Xianglin 14' were similar but were significantly greater than in 'Hengchong 89'. The net photosynthetic rate of 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was higher than 'Hengchong 89'. Pearson's correlation analysis showed that seed yields and oil yields were highly correlated with the expression level of Co-rbcL at P < 0.001 level; and the expression of Co-rbcS was correlated with oil yield at P < 0.01 level. Net photosynthetic rate was also correlated with oil yields and seed yields at P < 0.001 and P < 0.01 levels, respectively. Our results suggest that Co-rbcS and Co-rbcL in particular could potentially be molecular markers for early selection of high oil yield cultivars. In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency.

No MeSH data available.


Alignment of the amino acid sequence of rbcS from Camellia oleifera with the sequence from Camellia sinensis, Gossypium hirsutum, Pyrus pyrifolia, Brassica napus, and Arabidopsis thaliana. A. thaliana 1B, 2B, and 3B represent A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658), and A. thaliana small chain 3B (NP_198657), respectively. Amino acid residues identical to those of C. oleifera rbcS protein are indicated by dots. Hyphens indicate gaps. The arrows indicate the possible processing site of mature protein in C. oleifera.
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Figure 6: Alignment of the amino acid sequence of rbcS from Camellia oleifera with the sequence from Camellia sinensis, Gossypium hirsutum, Pyrus pyrifolia, Brassica napus, and Arabidopsis thaliana. A. thaliana 1B, 2B, and 3B represent A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658), and A. thaliana small chain 3B (NP_198657), respectively. Amino acid residues identical to those of C. oleifera rbcS protein are indicated by dots. Hyphens indicate gaps. The arrows indicate the possible processing site of mature protein in C. oleifera.

Mentions: Two strong transmembrane helices, signal peptides or transmembrane regions were found in the putative polypeptide of Co-rbcS by SignalP 3.0 Server using neural networks (NNs) and hidden Markov models (HMMs) trained on eukaryotes (Figure 6). The most likely cleavage site appeared to be between positions 16 and 17. Acidic residues and basic residues account for 25.5 and 12.25%, respectively. Hydrophobic residues and charged residues are 28.90 and 18.89%, respectively. Alignment of the putative Co-rbcS amino acid sequence with rbcS from the other plants showed that Co-rbcS shared 96% homology with that of C. sinensis (ABK15574), 78% homology with that of Gossypium hirsutum (AFS41732) and P. pyrifolia var. culta (BAA00450) rbcS, and 77% homology with that of B. napus rbcS (ABB51649) and A. thaliana small chain 3B (NP_198657) as well as 75 and 76% homology with A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658) and A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), respectively.


Identification of Rubisco rbcL and rbcS in Camellia oleifera and their potential as molecular markers for selection of high tea oil cultivars.

Chen Y, Wang B, Chen J, Wang X, Wang R, Peng S, Chen L, Ma L, Luo J - Front Plant Sci (2015)

Alignment of the amino acid sequence of rbcS from Camellia oleifera with the sequence from Camellia sinensis, Gossypium hirsutum, Pyrus pyrifolia, Brassica napus, and Arabidopsis thaliana. A. thaliana 1B, 2B, and 3B represent A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658), and A. thaliana small chain 3B (NP_198657), respectively. Amino acid residues identical to those of C. oleifera rbcS protein are indicated by dots. Hyphens indicate gaps. The arrows indicate the possible processing site of mature protein in C. oleifera.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379756&req=5

Figure 6: Alignment of the amino acid sequence of rbcS from Camellia oleifera with the sequence from Camellia sinensis, Gossypium hirsutum, Pyrus pyrifolia, Brassica napus, and Arabidopsis thaliana. A. thaliana 1B, 2B, and 3B represent A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658), and A. thaliana small chain 3B (NP_198657), respectively. Amino acid residues identical to those of C. oleifera rbcS protein are indicated by dots. Hyphens indicate gaps. The arrows indicate the possible processing site of mature protein in C. oleifera.
Mentions: Two strong transmembrane helices, signal peptides or transmembrane regions were found in the putative polypeptide of Co-rbcS by SignalP 3.0 Server using neural networks (NNs) and hidden Markov models (HMMs) trained on eukaryotes (Figure 6). The most likely cleavage site appeared to be between positions 16 and 17. Acidic residues and basic residues account for 25.5 and 12.25%, respectively. Hydrophobic residues and charged residues are 28.90 and 18.89%, respectively. Alignment of the putative Co-rbcS amino acid sequence with rbcS from the other plants showed that Co-rbcS shared 96% homology with that of C. sinensis (ABK15574), 78% homology with that of Gossypium hirsutum (AFS41732) and P. pyrifolia var. culta (BAA00450) rbcS, and 77% homology with that of B. napus rbcS (ABB51649) and A. thaliana small chain 3B (NP_198657) as well as 75 and 76% homology with A. thaliana ribulose bisphosphate carboxylase 2B small chain (NP_198658) and A. thaliana ribulose bisphosphate carboxylase small chain 1B (NP_198659), respectively.

Bottom Line: The Co-rbcL expression in 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was greater than 'Hengchong 89'.The expression levels of Co-rbcS in 'Xianglin 1' and 'Xianglin 14' were similar but were significantly greater than in 'Hengchong 89'.In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Technology Research Center of Oil-tea Camellia, Hunan Academy of Forestry Changsha, China.

ABSTRACT
Tea oil derived from seeds of Camellia oleifera Abel. is high-quality edible oil in China. This study isolated full-length cDNAs of Rubisco subunits rbcL and rbcS from C. oleifera. The rbcL has 1,522 bp with a 1,425 bp coding region, encoding 475 amino acids; and the rbcS has 615 bp containing a 528 bp coding region, encoding 176 amino acids. The expression level of the two genes, designated as Co-rbcL and Co-rbcS, was determined in three C. oleifera cultivars: Hengchong 89, Xianglin 1, and Xianglin 14 whose annual oil yields were 546.9, 591.4, and 657.7 kg ha(-1), respectively. The Co-rbcL expression in 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was greater than 'Hengchong 89'. The expression levels of Co-rbcS in 'Xianglin 1' and 'Xianglin 14' were similar but were significantly greater than in 'Hengchong 89'. The net photosynthetic rate of 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was higher than 'Hengchong 89'. Pearson's correlation analysis showed that seed yields and oil yields were highly correlated with the expression level of Co-rbcL at P < 0.001 level; and the expression of Co-rbcS was correlated with oil yield at P < 0.01 level. Net photosynthetic rate was also correlated with oil yields and seed yields at P < 0.001 and P < 0.01 levels, respectively. Our results suggest that Co-rbcS and Co-rbcL in particular could potentially be molecular markers for early selection of high oil yield cultivars. In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency.

No MeSH data available.