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Effect of technique of sealer agitation on percentage and depth of MTA Fillapex sealer penetration: A comparative in-vitro study.

Nikhil V, Bansal P, Sawani S - J Conserv Dent (2015 Mar-Apr)

Bottom Line: Statistical analysis was performed utilizing Kruskal-Wallis and Mann-Whitney U tests with a significance level of 5%.G1 showed significantly (P < 0.001) high percentage and depth of sealer penetration than G2 and G3 while the difference was insignificant (P > 0.05) between G2 and G3.Ultrasonic method of sealer activation and MTA Fillapex showed the best results.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Subharti Dental College, Meerut, Uttar Pradesh, India.

ABSTRACT

Aim: To compare the effect of three root canal sealer activation techniques on percentage and depth of sealer penetration of MTA Fillapex and AH Plus sealers.

Materials and methods: Sixty teeth prepared till F5 ProTaper size were divided into three equal groups on the basis of sealer activation technique (G1: Ultrasonics, G2: Lentulo spiral, and G3: Counter-clockwise rotary motion). Each group was further divided into two equal subgroups on the basis of type of sealer used: AH Plus (Denstply, Konstanz, Germany) or MTA Fillapex (Angelus, Londrina, PR, Brazil) and obturated with gutta-percha. Horizontal sections at 3 and 6 mm from the apex were obtained and the percentage and depth of penetration of sealers into dentinal tubules were measured using confocal laser scanning microscopy (CLSM). Statistical analysis was performed utilizing Kruskal-Wallis and Mann-Whitney U tests with a significance level of 5%.

Results: G1 showed significantly (P < 0.001) high percentage and depth of sealer penetration than G2 and G3 while the difference was insignificant (P > 0.05) between G2 and G3.

Conclusion: Percentage and depth of sealer penetration are influenced by the type of sealer used sealer activation technique and by the root canal level. Ultrasonic method of sealer activation and MTA Fillapex showed the best results.

No MeSH data available.


Related in: MedlinePlus

Confocal laser scanning microscopic photograph showing calculation of percentage of penetration of sealer in the dentinal tubules (original magnification 10×)
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Figure 1: Confocal laser scanning microscopic photograph showing calculation of percentage of penetration of sealer in the dentinal tubules (original magnification 10×)

Mentions: Each root was sectioned at 90-° to the long axis by using diamond disc removing two, 1 mm sections at 3 mm and 6 mm from the apex. Coronal surface of each section was polished with sand paper (Politriz, Arotec, Cotia, SP, Brazil). The dentin segments were examined on a confocal microscope (Olympus Fluoview FV 1000). The respective absorption and emission wave lengths for the Rhodomine B were 540 nm and 590 nm. Dentin samples were analyzed using the 10X lens. To calculate the percentage of sealer penetration around the root canal, first each image was imported into the IOB software and the circumference of root canal measured using its ruler tool. Next, areas along the canal walls in which the sealer penetrated into dentinal tubules were outlined and measured using the same method [Figure 1]. Subsequently, the percentage of root canal sealer penetration in that section was established. To determine the maximum depth of penetration, the point of deepest penetration was measured from the canal wall to the maximum depth of penetration [Figure 2]. The effects of sealer agitation techniques and type of sealer on depth and percentage of sealer penetration were analyzed, by performing nonparametric Kruskal-Wallis for overall analysis, and a series of Mann-Whitney U tests for pairwise comparison using statistical package of social sciences (SPSS) statistics 20 software.


Effect of technique of sealer agitation on percentage and depth of MTA Fillapex sealer penetration: A comparative in-vitro study.

Nikhil V, Bansal P, Sawani S - J Conserv Dent (2015 Mar-Apr)

Confocal laser scanning microscopic photograph showing calculation of percentage of penetration of sealer in the dentinal tubules (original magnification 10×)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379650&req=5

Figure 1: Confocal laser scanning microscopic photograph showing calculation of percentage of penetration of sealer in the dentinal tubules (original magnification 10×)
Mentions: Each root was sectioned at 90-° to the long axis by using diamond disc removing two, 1 mm sections at 3 mm and 6 mm from the apex. Coronal surface of each section was polished with sand paper (Politriz, Arotec, Cotia, SP, Brazil). The dentin segments were examined on a confocal microscope (Olympus Fluoview FV 1000). The respective absorption and emission wave lengths for the Rhodomine B were 540 nm and 590 nm. Dentin samples were analyzed using the 10X lens. To calculate the percentage of sealer penetration around the root canal, first each image was imported into the IOB software and the circumference of root canal measured using its ruler tool. Next, areas along the canal walls in which the sealer penetrated into dentinal tubules were outlined and measured using the same method [Figure 1]. Subsequently, the percentage of root canal sealer penetration in that section was established. To determine the maximum depth of penetration, the point of deepest penetration was measured from the canal wall to the maximum depth of penetration [Figure 2]. The effects of sealer agitation techniques and type of sealer on depth and percentage of sealer penetration were analyzed, by performing nonparametric Kruskal-Wallis for overall analysis, and a series of Mann-Whitney U tests for pairwise comparison using statistical package of social sciences (SPSS) statistics 20 software.

Bottom Line: Statistical analysis was performed utilizing Kruskal-Wallis and Mann-Whitney U tests with a significance level of 5%.G1 showed significantly (P < 0.001) high percentage and depth of sealer penetration than G2 and G3 while the difference was insignificant (P > 0.05) between G2 and G3.Ultrasonic method of sealer activation and MTA Fillapex showed the best results.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Subharti Dental College, Meerut, Uttar Pradesh, India.

ABSTRACT

Aim: To compare the effect of three root canal sealer activation techniques on percentage and depth of sealer penetration of MTA Fillapex and AH Plus sealers.

Materials and methods: Sixty teeth prepared till F5 ProTaper size were divided into three equal groups on the basis of sealer activation technique (G1: Ultrasonics, G2: Lentulo spiral, and G3: Counter-clockwise rotary motion). Each group was further divided into two equal subgroups on the basis of type of sealer used: AH Plus (Denstply, Konstanz, Germany) or MTA Fillapex (Angelus, Londrina, PR, Brazil) and obturated with gutta-percha. Horizontal sections at 3 and 6 mm from the apex were obtained and the percentage and depth of penetration of sealers into dentinal tubules were measured using confocal laser scanning microscopy (CLSM). Statistical analysis was performed utilizing Kruskal-Wallis and Mann-Whitney U tests with a significance level of 5%.

Results: G1 showed significantly (P < 0.001) high percentage and depth of sealer penetration than G2 and G3 while the difference was insignificant (P > 0.05) between G2 and G3.

Conclusion: Percentage and depth of sealer penetration are influenced by the type of sealer used sealer activation technique and by the root canal level. Ultrasonic method of sealer activation and MTA Fillapex showed the best results.

No MeSH data available.


Related in: MedlinePlus