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An optical method for serum calcium and phosphorus level assessment during hemodialysis.

Holmar J, Uhlin F, Fernström A, Luman M, Jankowski J, Fridolin I - Toxins (Basel) (2015)

Bottom Line: Obtained levels were compared in means and SD.The levels achieved were not significantly different (p = 0.5).In conclusion, optical monitoring of the spent dialysate for assessing the serum levels of Ca and P during dialysis seems to be feasible and could offer valuable and continuous information to medical staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Technomedicum, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia. jana@cb.ttu.ee.

ABSTRACT
Survival among hemodialysis patients is disturbingly low, partly because vascular calcification (VC) and cardiovascular disease are highly prevalent. Elevated serum phosphorus (P) and calcium (Ca) levels play an essential role in the formation of VC events. The purpose of the current study was to reveal optical monitoring possibilities of serum P and Ca values during dialysis. Twenty-eight patients from Tallinn (Estonia) and Linköping (Sweden) were included in the study. The serum levels of Ca and P on the basis of optical information, i.e., absorbance and fluorescence of the spent dialysate (optical method) were assessed. Obtained levels were compared in means and SD. The mean serum level of Ca was 2.54 ± 0.21 and 2.53 ± 0.19 mmol/L; P levels varied between 1.08 ± 0.51 and 1.08 ± 0.48 mmol/L, measured in the laboratory and estimated by the optical method respectively. The levels achieved were not significantly different (p = 0.5). The Bland-Altman 95% limits of agreement between the two methods varied from -0.19 to 0.19 for Ca and from -0.37 to 0.37 in the case of P. In conclusion, optical monitoring of the spent dialysate for assessing the serum levels of Ca and P during dialysis seems to be feasible and could offer valuable and continuous information to medical staff.

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Related in: MedlinePlus

Examples of fluorescence spectra of spent dialysate taken at the start (10 min) and at the end (240 min) of the dialysis procedure. Excitation (EX) = 220–500 nm, Emission (EM) = 220–500 nm.
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toxins-07-00719-f001: Examples of fluorescence spectra of spent dialysate taken at the start (10 min) and at the end (240 min) of the dialysis procedure. Excitation (EX) = 220–500 nm, Emission (EM) = 220–500 nm.

Mentions: Fluorescence analysis was performed over an excitation (EX) wavelength range of 220–500 nm (excitation increment 10 nm); emission (EM) spectra over a wavelength range of 220–500 nm were recorded at each excitation and the resulting 3D fluorescence maps are presented in Figure 1. The amplitude of the spectra is proportional to the content of eliminated uremic retention solutes in the spent dialysate.


An optical method for serum calcium and phosphorus level assessment during hemodialysis.

Holmar J, Uhlin F, Fernström A, Luman M, Jankowski J, Fridolin I - Toxins (Basel) (2015)

Examples of fluorescence spectra of spent dialysate taken at the start (10 min) and at the end (240 min) of the dialysis procedure. Excitation (EX) = 220–500 nm, Emission (EM) = 220–500 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379520&req=5

toxins-07-00719-f001: Examples of fluorescence spectra of spent dialysate taken at the start (10 min) and at the end (240 min) of the dialysis procedure. Excitation (EX) = 220–500 nm, Emission (EM) = 220–500 nm.
Mentions: Fluorescence analysis was performed over an excitation (EX) wavelength range of 220–500 nm (excitation increment 10 nm); emission (EM) spectra over a wavelength range of 220–500 nm were recorded at each excitation and the resulting 3D fluorescence maps are presented in Figure 1. The amplitude of the spectra is proportional to the content of eliminated uremic retention solutes in the spent dialysate.

Bottom Line: Obtained levels were compared in means and SD.The levels achieved were not significantly different (p = 0.5).In conclusion, optical monitoring of the spent dialysate for assessing the serum levels of Ca and P during dialysis seems to be feasible and could offer valuable and continuous information to medical staff.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Technomedicum, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia. jana@cb.ttu.ee.

ABSTRACT
Survival among hemodialysis patients is disturbingly low, partly because vascular calcification (VC) and cardiovascular disease are highly prevalent. Elevated serum phosphorus (P) and calcium (Ca) levels play an essential role in the formation of VC events. The purpose of the current study was to reveal optical monitoring possibilities of serum P and Ca values during dialysis. Twenty-eight patients from Tallinn (Estonia) and Linköping (Sweden) were included in the study. The serum levels of Ca and P on the basis of optical information, i.e., absorbance and fluorescence of the spent dialysate (optical method) were assessed. Obtained levels were compared in means and SD. The mean serum level of Ca was 2.54 ± 0.21 and 2.53 ± 0.19 mmol/L; P levels varied between 1.08 ± 0.51 and 1.08 ± 0.48 mmol/L, measured in the laboratory and estimated by the optical method respectively. The levels achieved were not significantly different (p = 0.5). The Bland-Altman 95% limits of agreement between the two methods varied from -0.19 to 0.19 for Ca and from -0.37 to 0.37 in the case of P. In conclusion, optical monitoring of the spent dialysate for assessing the serum levels of Ca and P during dialysis seems to be feasible and could offer valuable and continuous information to medical staff.

Show MeSH
Related in: MedlinePlus