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Preliminary estimation of deoxynivalenol excretion through a 24 h pilot study.

Rodríguez-Carrasco Y, Mañes J, Berrada H, Font G - Toxins (Basel) (2015)

Bottom Line: Satisfactory results in method performance were achieved.The matrix effect was evaluated and matrix-matched calibrations were used for quantitation.Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

View Article: PubMed Central - PubMed

Affiliation: Area of Toxicology, Department of Public Health, Faculty of Pharmacy, University of Valencia, Burjassot 46100, Spain. yelko.rodriguez@uv.es.

ABSTRACT
A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method's accuracy was in a range of 68%-108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

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Selected reaction monitoring (SRM) chromatograms of the naturally deoxynivalenol (DON) contaminated pasta before and after culinary treatment at 140.7 µg/kg and 60.1 µg/kg, respectively, on a dry weight basis, and percentage of mycotoxin reduction.
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toxins-07-00705-f001: Selected reaction monitoring (SRM) chromatograms of the naturally deoxynivalenol (DON) contaminated pasta before and after culinary treatment at 140.7 µg/kg and 60.1 µg/kg, respectively, on a dry weight basis, and percentage of mycotoxin reduction.

Mentions: Mycotoxin analyses of pasta (spaghetti) and whole-wheat pasta (little stars) were carried out prior the cooking step and then cooked after the drying process. Analyses were performed in triplicate. The purpose was to evaluate the percentage of mycotoxin reduction during food preparation procedure. Most of the exposure assessment approach to contaminants has been carried out based on un-cooked food, and thus assuming some uncertainty in the reported data. In this work, not only cooked meals but also the regular cooking practices and the serving size were taking into account. The aim was not to serve as a representative data of percentage of reduction for wheat-based products, but to minimize the uncertainty of the obtained data in order to allow a closer exposure assessment approach. A reduction of 13% and 58% was obtained for whole-wheat pasta and pasta, respectively after culinary treatment (Figure 1). That difference could be attributed to the distinct serving. While the cooking water is removed in spaghetti serving, little stars are consumed as a soup and thus, the amount of mycotoxin intake in little stars was higher than in spaghetti. The percentage of reduction obtained in this work were comparable than those reported Visconti et al. [10] in a larger study showing average DON reduction levels of 40%. Moreover, they indicated that the amount of DON retained by cooked pasta consistently decreased by increasing the pasta/water ratio during cooking.


Preliminary estimation of deoxynivalenol excretion through a 24 h pilot study.

Rodríguez-Carrasco Y, Mañes J, Berrada H, Font G - Toxins (Basel) (2015)

Selected reaction monitoring (SRM) chromatograms of the naturally deoxynivalenol (DON) contaminated pasta before and after culinary treatment at 140.7 µg/kg and 60.1 µg/kg, respectively, on a dry weight basis, and percentage of mycotoxin reduction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379519&req=5

toxins-07-00705-f001: Selected reaction monitoring (SRM) chromatograms of the naturally deoxynivalenol (DON) contaminated pasta before and after culinary treatment at 140.7 µg/kg and 60.1 µg/kg, respectively, on a dry weight basis, and percentage of mycotoxin reduction.
Mentions: Mycotoxin analyses of pasta (spaghetti) and whole-wheat pasta (little stars) were carried out prior the cooking step and then cooked after the drying process. Analyses were performed in triplicate. The purpose was to evaluate the percentage of mycotoxin reduction during food preparation procedure. Most of the exposure assessment approach to contaminants has been carried out based on un-cooked food, and thus assuming some uncertainty in the reported data. In this work, not only cooked meals but also the regular cooking practices and the serving size were taking into account. The aim was not to serve as a representative data of percentage of reduction for wheat-based products, but to minimize the uncertainty of the obtained data in order to allow a closer exposure assessment approach. A reduction of 13% and 58% was obtained for whole-wheat pasta and pasta, respectively after culinary treatment (Figure 1). That difference could be attributed to the distinct serving. While the cooking water is removed in spaghetti serving, little stars are consumed as a soup and thus, the amount of mycotoxin intake in little stars was higher than in spaghetti. The percentage of reduction obtained in this work were comparable than those reported Visconti et al. [10] in a larger study showing average DON reduction levels of 40%. Moreover, they indicated that the amount of DON retained by cooked pasta consistently decreased by increasing the pasta/water ratio during cooking.

Bottom Line: Satisfactory results in method performance were achieved.The matrix effect was evaluated and matrix-matched calibrations were used for quantitation.Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

View Article: PubMed Central - PubMed

Affiliation: Area of Toxicology, Department of Public Health, Faculty of Pharmacy, University of Valencia, Burjassot 46100, Spain. yelko.rodriguez@uv.es.

ABSTRACT
A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method's accuracy was in a range of 68%-108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

Show MeSH