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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM - Elife (2015)

Bottom Line: The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research.In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue.We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France.

ABSTRACT
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

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Absorption and emission spectra of CaRu-Nano.DOI:http://dx.doi.org/10.7554/eLife.05808.005
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fig1s2: Absorption and emission spectra of CaRu-Nano.DOI:http://dx.doi.org/10.7554/eLife.05808.005


CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM - Elife (2015)

Absorption and emission spectra of CaRu-Nano.DOI:http://dx.doi.org/10.7554/eLife.05808.005
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379494&req=5

fig1s2: Absorption and emission spectra of CaRu-Nano.DOI:http://dx.doi.org/10.7554/eLife.05808.005
Bottom Line: The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research.In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue.We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France.

ABSTRACT
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

Show MeSH