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Oldenlandia diffusa Promotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis.

Sunwoo YY, Lee JH, Jung HY, Jung YJ, Park MS, Chung YA, Maeng LS, Han YM, Shin HS, Lee J, Park SI - Evid Based Complement Alternat Med (2015)

Bottom Line: Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model.OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells.These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.

View Article: PubMed Central - PubMed

Affiliation: Comprehensive Hospital for Advanced Cancer, International St. Mary's Hospital, College of Medicine, The Catholic Kwandong University of Korea, Incheon 404-834, Republic of Korea.

ABSTRACT
Oldenlandia diffusa (OD) is commonly used with various diseases such as cancer, arthritis, and autoimmune disease. Liver cirrhosis is a predominant risk factor for hepatocellular carcinoma (HCC). Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model. OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells. In vivo, OD was treated twice a day for 28 days after confirmed HCC model through 2-[(18)F]-fluoro-2-deoxy-D-glucose ((18)F-FDG) imaging. The survival in OD treated groups was shown to have a greater therapeutic effect than the control group. 28 days after OD treatment, OD treated groups resulted in a significant reduction in tumor number, size, (18)F-FDG uptake, and serum levels such as alanine transaminase, aspartate transaminase, and alkaline phosphate compared to the control group. Also, proliferated cells in tumor sites by OD were reduced compared to the control group. Furthermore, several rats in OD treated group survived over 60 days and liver morphology of these rats showed the difference between tumor mass and normal tissue. These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.

No MeSH data available.


Related in: MedlinePlus

The analysis of level on serum, tumor number categorized by tumor size, and assessment of the liver/body weight 28 days after OD treatment. The blood samples were collected 28 days after OD treatment and the serum level of ALP, AST, and ALT was measured (a). Comparison of tumor number was categorized by tumor size 28 days after OD treatment (b). Also, liver/body weight ratio was measured 28 days after OD treatment. The serum levels of ALP, AST, and ALT in OD treated group were significantly decreased compared to the control group (a). Also, a larger tumor burden in OD treated group was observed less than control group (b). The liver/body weight ratio in rats after OD treatment (c). OD treated group significantly reduced the liver/body ratio compared to the control group. Columns, mean; bar, SD. *P < 0.05 and **P < 0.01.
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fig4: The analysis of level on serum, tumor number categorized by tumor size, and assessment of the liver/body weight 28 days after OD treatment. The blood samples were collected 28 days after OD treatment and the serum level of ALP, AST, and ALT was measured (a). Comparison of tumor number was categorized by tumor size 28 days after OD treatment (b). Also, liver/body weight ratio was measured 28 days after OD treatment. The serum levels of ALP, AST, and ALT in OD treated group were significantly decreased compared to the control group (a). Also, a larger tumor burden in OD treated group was observed less than control group (b). The liver/body weight ratio in rats after OD treatment (c). OD treated group significantly reduced the liver/body ratio compared to the control group. Columns, mean; bar, SD. *P < 0.05 and **P < 0.01.

Mentions: We investigated whether OD could change hepatic function related factors including ALT, AST, and ALP on serum level in DEN-induced HCC model. We collected serum 28 days after OD treatment and analyzed component of related liver function. Also, a comparison was made by categorizing the tumor number by tumor size and measured the liver/body ratio 28 days after OD treatment. The serum ALP, AST, and ALT levels in OD treated group (100 and 200 mg/kg) were significantly lower compared to the control group (Figure 4(a)). Also, the size distribution of nodules and the liver/body ratio in OD treated group (100 and 200 mg/kg) were significantly reduced compared to the control group (Figures 4(b) and 4(c)). These results suggest that OD may regulate the development and metastasis in tumors by enhancing hepatic function.


Oldenlandia diffusa Promotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis.

Sunwoo YY, Lee JH, Jung HY, Jung YJ, Park MS, Chung YA, Maeng LS, Han YM, Shin HS, Lee J, Park SI - Evid Based Complement Alternat Med (2015)

The analysis of level on serum, tumor number categorized by tumor size, and assessment of the liver/body weight 28 days after OD treatment. The blood samples were collected 28 days after OD treatment and the serum level of ALP, AST, and ALT was measured (a). Comparison of tumor number was categorized by tumor size 28 days after OD treatment (b). Also, liver/body weight ratio was measured 28 days after OD treatment. The serum levels of ALP, AST, and ALT in OD treated group were significantly decreased compared to the control group (a). Also, a larger tumor burden in OD treated group was observed less than control group (b). The liver/body weight ratio in rats after OD treatment (c). OD treated group significantly reduced the liver/body ratio compared to the control group. Columns, mean; bar, SD. *P < 0.05 and **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: The analysis of level on serum, tumor number categorized by tumor size, and assessment of the liver/body weight 28 days after OD treatment. The blood samples were collected 28 days after OD treatment and the serum level of ALP, AST, and ALT was measured (a). Comparison of tumor number was categorized by tumor size 28 days after OD treatment (b). Also, liver/body weight ratio was measured 28 days after OD treatment. The serum levels of ALP, AST, and ALT in OD treated group were significantly decreased compared to the control group (a). Also, a larger tumor burden in OD treated group was observed less than control group (b). The liver/body weight ratio in rats after OD treatment (c). OD treated group significantly reduced the liver/body ratio compared to the control group. Columns, mean; bar, SD. *P < 0.05 and **P < 0.01.
Mentions: We investigated whether OD could change hepatic function related factors including ALT, AST, and ALP on serum level in DEN-induced HCC model. We collected serum 28 days after OD treatment and analyzed component of related liver function. Also, a comparison was made by categorizing the tumor number by tumor size and measured the liver/body ratio 28 days after OD treatment. The serum ALP, AST, and ALT levels in OD treated group (100 and 200 mg/kg) were significantly lower compared to the control group (Figure 4(a)). Also, the size distribution of nodules and the liver/body ratio in OD treated group (100 and 200 mg/kg) were significantly reduced compared to the control group (Figures 4(b) and 4(c)). These results suggest that OD may regulate the development and metastasis in tumors by enhancing hepatic function.

Bottom Line: Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model.OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells.These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.

View Article: PubMed Central - PubMed

Affiliation: Comprehensive Hospital for Advanced Cancer, International St. Mary's Hospital, College of Medicine, The Catholic Kwandong University of Korea, Incheon 404-834, Republic of Korea.

ABSTRACT
Oldenlandia diffusa (OD) is commonly used with various diseases such as cancer, arthritis, and autoimmune disease. Liver cirrhosis is a predominant risk factor for hepatocellular carcinoma (HCC). Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model. OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells. In vivo, OD was treated twice a day for 28 days after confirmed HCC model through 2-[(18)F]-fluoro-2-deoxy-D-glucose ((18)F-FDG) imaging. The survival in OD treated groups was shown to have a greater therapeutic effect than the control group. 28 days after OD treatment, OD treated groups resulted in a significant reduction in tumor number, size, (18)F-FDG uptake, and serum levels such as alanine transaminase, aspartate transaminase, and alkaline phosphate compared to the control group. Also, proliferated cells in tumor sites by OD were reduced compared to the control group. Furthermore, several rats in OD treated group survived over 60 days and liver morphology of these rats showed the difference between tumor mass and normal tissue. These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.

No MeSH data available.


Related in: MedlinePlus