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Ribonuclease J is required for chloroplast and embryo development in Arabidopsis.

Chen H, Zou W, Zhao J - J. Exp. Bot. (2015)

Bottom Line: Furthermore, by analyzing the molecular markers of cell fate decisions (STM, FIL, ML1, SCR, and WOX5) in rnj embryos, we found that this impairment of chloroplast development may lead to aberrant embryo patterning along the apical-basal axis, indicating that AtRNJ is important in initiating and maintaining the organization of shoot apical meristems (SAMs), cotyledons, and hypocotyls.Moreover, the transport and response of auxin in rnj embryos was found to be disrupted, suggesting that AtRNJ may be involved in auxin-mediated pathways during embryogenesis.Therefore, we speculate that RNJ plays a vital role in embryo morphogenesis and apical-basal pattern formation by regulating chloroplast development.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.

No MeSH data available.


Related in: MedlinePlus

TEM of chloroplast development in wild-type and rnj-2 embryo cells. (A) Cells in wild-type embryos at 5 DAP. (B) Cells in rnj-2 embryos at 5 DAP. Asterisk indicates a normal chloroplast in the wild type; star indicates an impaired chloroplast in the rnj-2 mutant. N, nucleolus; Nu, nucleus; CW, cell wall. Scale bars = 1 μm.
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Figure 4: TEM of chloroplast development in wild-type and rnj-2 embryo cells. (A) Cells in wild-type embryos at 5 DAP. (B) Cells in rnj-2 embryos at 5 DAP. Asterisk indicates a normal chloroplast in the wild type; star indicates an impaired chloroplast in the rnj-2 mutant. N, nucleolus; Nu, nucleus; CW, cell wall. Scale bars = 1 μm.

Mentions: Since the rnj heterozygous mutants produced about 25% albino seeds in siliques, we wondered whether the homozygous embryo lethality was due to impaired chloroplast development in the mutant embryo cells. We prepared wild-type and rnj albino ovules from 5 DAP siliques as samples for ultrastructural observation with TEM. In the embryo wild-type samples, chloroplasts had organized thylakoid membranes stacked into grana that were well developed (Fig. 4A). However, in the rnj embryo samples, we observed many immature plastids that lacked internal thylakoid membranes but contained darkly stained aggregations (Fig. 4B). The lack of normal chloroplasts in the mutant embryo cells indicated that the rnj mutation may disturb the formation of internal thylakoid membranes during embryo development and lead to impaired chloroplasts, strongly suggesting that the RNJ gene is required for chloroplast development.


Ribonuclease J is required for chloroplast and embryo development in Arabidopsis.

Chen H, Zou W, Zhao J - J. Exp. Bot. (2015)

TEM of chloroplast development in wild-type and rnj-2 embryo cells. (A) Cells in wild-type embryos at 5 DAP. (B) Cells in rnj-2 embryos at 5 DAP. Asterisk indicates a normal chloroplast in the wild type; star indicates an impaired chloroplast in the rnj-2 mutant. N, nucleolus; Nu, nucleus; CW, cell wall. Scale bars = 1 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4378637&req=5

Figure 4: TEM of chloroplast development in wild-type and rnj-2 embryo cells. (A) Cells in wild-type embryos at 5 DAP. (B) Cells in rnj-2 embryos at 5 DAP. Asterisk indicates a normal chloroplast in the wild type; star indicates an impaired chloroplast in the rnj-2 mutant. N, nucleolus; Nu, nucleus; CW, cell wall. Scale bars = 1 μm.
Mentions: Since the rnj heterozygous mutants produced about 25% albino seeds in siliques, we wondered whether the homozygous embryo lethality was due to impaired chloroplast development in the mutant embryo cells. We prepared wild-type and rnj albino ovules from 5 DAP siliques as samples for ultrastructural observation with TEM. In the embryo wild-type samples, chloroplasts had organized thylakoid membranes stacked into grana that were well developed (Fig. 4A). However, in the rnj embryo samples, we observed many immature plastids that lacked internal thylakoid membranes but contained darkly stained aggregations (Fig. 4B). The lack of normal chloroplasts in the mutant embryo cells indicated that the rnj mutation may disturb the formation of internal thylakoid membranes during embryo development and lead to impaired chloroplasts, strongly suggesting that the RNJ gene is required for chloroplast development.

Bottom Line: Furthermore, by analyzing the molecular markers of cell fate decisions (STM, FIL, ML1, SCR, and WOX5) in rnj embryos, we found that this impairment of chloroplast development may lead to aberrant embryo patterning along the apical-basal axis, indicating that AtRNJ is important in initiating and maintaining the organization of shoot apical meristems (SAMs), cotyledons, and hypocotyls.Moreover, the transport and response of auxin in rnj embryos was found to be disrupted, suggesting that AtRNJ may be involved in auxin-mediated pathways during embryogenesis.Therefore, we speculate that RNJ plays a vital role in embryo morphogenesis and apical-basal pattern formation by regulating chloroplast development.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.

No MeSH data available.


Related in: MedlinePlus