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The identification of new cytosolic glutamine synthetase and asparagine synthetase genes in barley (Hordeum vulgare L.), and their expression during leaf senescence.

Avila-Ospina L, Marmagne A, Talbotec J, Krupinska K, Masclaux-Daubresse C - J. Exp. Bot. (2015)

Bottom Line: The three eukaryotic-like HvGS1_1, HvGS1_2, and HvGS1_3 sequences showed the typical senescence-induced reduction in gene expression described in many plant species.By contrast, the two prokaryotic-like HvGS1_4 and HvGS1_5 sequences were repressed by leaf senescence, similar to the HvGS2 gene, which encodes the chloroplast glutamine synthetase isoenzyme.Responses of the HvASN sequences to dark-induced senescence showed that there are two categories of asparagine synthetases, one induced in the dark and the other repressed by the same conditions.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France.

No MeSH data available.


Transcript levels of HvGS, HvASN, and HvNAC13 genes in flag leaves harvested at different stages of senescence. Young leaves (T1), mature leaves (T3), and senescing leaves (T5) were analysed. Log10 relative expression values are shown. Data are mean ±SD (n=3 biological replicates). The ns and asterisks (*) indicate, respectively, the non-significant and significant differences occurring during ageing, i.e. between T3 and T1 and between T5 and T3. Significance was evaluated using a t test with P <0.05. HvActin was used as the reference gene.
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Figure 7: Transcript levels of HvGS, HvASN, and HvNAC13 genes in flag leaves harvested at different stages of senescence. Young leaves (T1), mature leaves (T3), and senescing leaves (T5) were analysed. Log10 relative expression values are shown. Data are mean ±SD (n=3 biological replicates). The ns and asterisks (*) indicate, respectively, the non-significant and significant differences occurring during ageing, i.e. between T3 and T1 and between T5 and T3. Significance was evaluated using a t test with P <0.05. HvActin was used as the reference gene.

Mentions: Unfortunately, and certainly because of gene polymorphisms between Golden Promise and Carina genotypes, the expression of only HvGS1_1, HvGS1_4, HvGS1_5, HvGS2, HvASN3, and HvASN4 could be monitored in the flag leaf samples. The primers identified for the other HvGS and HvASN genes on the Golden Promise genomic sequence could not be used for real-time RT-qPCR on Carina samples. The HvGS1_1 and HvGS1_4 transcript levels gradually increased from T1 to T5 in parallel with HvNAC13, which was opposite to the HvGS2 transcripts (Fig. 7). HvGS1_5 gene expression did not change with ageing. The HvASN3 mRNA level slightly increased in old leaves (T3 and T5) compared with young ones (T1). HvASN4 gene expression decreased from T1 to T5 showing a completely opposite pattern to HvASN3 (Fig. 7).


The identification of new cytosolic glutamine synthetase and asparagine synthetase genes in barley (Hordeum vulgare L.), and their expression during leaf senescence.

Avila-Ospina L, Marmagne A, Talbotec J, Krupinska K, Masclaux-Daubresse C - J. Exp. Bot. (2015)

Transcript levels of HvGS, HvASN, and HvNAC13 genes in flag leaves harvested at different stages of senescence. Young leaves (T1), mature leaves (T3), and senescing leaves (T5) were analysed. Log10 relative expression values are shown. Data are mean ±SD (n=3 biological replicates). The ns and asterisks (*) indicate, respectively, the non-significant and significant differences occurring during ageing, i.e. between T3 and T1 and between T5 and T3. Significance was evaluated using a t test with P <0.05. HvActin was used as the reference gene.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4378633&req=5

Figure 7: Transcript levels of HvGS, HvASN, and HvNAC13 genes in flag leaves harvested at different stages of senescence. Young leaves (T1), mature leaves (T3), and senescing leaves (T5) were analysed. Log10 relative expression values are shown. Data are mean ±SD (n=3 biological replicates). The ns and asterisks (*) indicate, respectively, the non-significant and significant differences occurring during ageing, i.e. between T3 and T1 and between T5 and T3. Significance was evaluated using a t test with P <0.05. HvActin was used as the reference gene.
Mentions: Unfortunately, and certainly because of gene polymorphisms between Golden Promise and Carina genotypes, the expression of only HvGS1_1, HvGS1_4, HvGS1_5, HvGS2, HvASN3, and HvASN4 could be monitored in the flag leaf samples. The primers identified for the other HvGS and HvASN genes on the Golden Promise genomic sequence could not be used for real-time RT-qPCR on Carina samples. The HvGS1_1 and HvGS1_4 transcript levels gradually increased from T1 to T5 in parallel with HvNAC13, which was opposite to the HvGS2 transcripts (Fig. 7). HvGS1_5 gene expression did not change with ageing. The HvASN3 mRNA level slightly increased in old leaves (T3 and T5) compared with young ones (T1). HvASN4 gene expression decreased from T1 to T5 showing a completely opposite pattern to HvASN3 (Fig. 7).

Bottom Line: The three eukaryotic-like HvGS1_1, HvGS1_2, and HvGS1_3 sequences showed the typical senescence-induced reduction in gene expression described in many plant species.By contrast, the two prokaryotic-like HvGS1_4 and HvGS1_5 sequences were repressed by leaf senescence, similar to the HvGS2 gene, which encodes the chloroplast glutamine synthetase isoenzyme.Responses of the HvASN sequences to dark-induced senescence showed that there are two categories of asparagine synthetases, one induced in the dark and the other repressed by the same conditions.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France.

No MeSH data available.