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The transcription factor PHR1 regulates lipid remodeling and triacylglycerol accumulation in Arabidopsis thaliana during phosphorus starvation.

Pant BD, Burgos A, Pant P, Cuadros-Inostroza A, Willmitzer L, Scheible WR - J. Exp. Bot. (2015)

Bottom Line: It consists of the degradation of phospholipids to release the phosphate needed by the cell and the accumulation of glycolipids to replace phospholipids in the membranes.Triacylglycerol (TAG) accumulates dramatically under P starvation (by as much as ~20-fold in shoots and ~13-fold in roots), a response known to occur in green algae but hardly known in plants.Surprisingly, there was an increase in phosphatidylglycerol (PG) in P-starved roots, a response that may be adaptive as it was suppressed in the phr1 mutant.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA.

No MeSH data available.


Related in: MedlinePlus

Expression of most lipid-remodeling genes during P starvation is under the control of PHR1. The influence of PHR1 appears stronger in the root according to qRT–PCR data. The values are expressed in 40-∆CTs, where ∆CT is the difference between the CT (threshold cycle number) of a tested gene and the reference gene (UBQ10, AT4G05320). The highest value possible is 40, as a qPCR run stops after completing 40 cycles. Values shown correspond to wild-type plants growing at 3mM Pi (black bars) and 0mM Pi (grey bars), and phr1 plants at 0mM Pi (dashed bars). Bar heights represent the average of two technical and two biological replicates. Error bars depict one SD.
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Figure 5: Expression of most lipid-remodeling genes during P starvation is under the control of PHR1. The influence of PHR1 appears stronger in the root according to qRT–PCR data. The values are expressed in 40-∆CTs, where ∆CT is the difference between the CT (threshold cycle number) of a tested gene and the reference gene (UBQ10, AT4G05320). The highest value possible is 40, as a qPCR run stops after completing 40 cycles. Values shown correspond to wild-type plants growing at 3mM Pi (black bars) and 0mM Pi (grey bars), and phr1 plants at 0mM Pi (dashed bars). Bar heights represent the average of two technical and two biological replicates. Error bars depict one SD.

Mentions: The response of the set of gene transcripts analyzed was different in shoots or roots (Fig. 5). In fact, the induction of practically all of them was stronger in the shoot than in the root. For instance, while NPC4 had an ~8.5-fold induction in the shoot, it was induced only by ~2.4-fold in the root (Fig. 5; Supplementary Table S3 at JXB online). The differential behaviour in transcriptional programmes between shoots and roots was already noticed by Woo et al. (2012), who showed that different regulons operate upon P starvation in the two parts of the plant. Interestingly, the effect of the phr1 mutation appeared to be more critical in the root even though these gene transcripts had a higher induction in the shoot. In the phr1 shoot, the genes were induced by more or less half the wild-type response (Fig. 5, Supplementary Table S3 at JXB online), while in the root the induction of most genes was practically abolished. Only four gene transcripts (PLDZ2, PLA2A, MGD3, and SQD1) were still found to be slightly induced in phr1 roots, none of them >2-fold Fig. 5, Supplementary Table S3 at JXB online.


The transcription factor PHR1 regulates lipid remodeling and triacylglycerol accumulation in Arabidopsis thaliana during phosphorus starvation.

Pant BD, Burgos A, Pant P, Cuadros-Inostroza A, Willmitzer L, Scheible WR - J. Exp. Bot. (2015)

Expression of most lipid-remodeling genes during P starvation is under the control of PHR1. The influence of PHR1 appears stronger in the root according to qRT–PCR data. The values are expressed in 40-∆CTs, where ∆CT is the difference between the CT (threshold cycle number) of a tested gene and the reference gene (UBQ10, AT4G05320). The highest value possible is 40, as a qPCR run stops after completing 40 cycles. Values shown correspond to wild-type plants growing at 3mM Pi (black bars) and 0mM Pi (grey bars), and phr1 plants at 0mM Pi (dashed bars). Bar heights represent the average of two technical and two biological replicates. Error bars depict one SD.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4378627&req=5

Figure 5: Expression of most lipid-remodeling genes during P starvation is under the control of PHR1. The influence of PHR1 appears stronger in the root according to qRT–PCR data. The values are expressed in 40-∆CTs, where ∆CT is the difference between the CT (threshold cycle number) of a tested gene and the reference gene (UBQ10, AT4G05320). The highest value possible is 40, as a qPCR run stops after completing 40 cycles. Values shown correspond to wild-type plants growing at 3mM Pi (black bars) and 0mM Pi (grey bars), and phr1 plants at 0mM Pi (dashed bars). Bar heights represent the average of two technical and two biological replicates. Error bars depict one SD.
Mentions: The response of the set of gene transcripts analyzed was different in shoots or roots (Fig. 5). In fact, the induction of practically all of them was stronger in the shoot than in the root. For instance, while NPC4 had an ~8.5-fold induction in the shoot, it was induced only by ~2.4-fold in the root (Fig. 5; Supplementary Table S3 at JXB online). The differential behaviour in transcriptional programmes between shoots and roots was already noticed by Woo et al. (2012), who showed that different regulons operate upon P starvation in the two parts of the plant. Interestingly, the effect of the phr1 mutation appeared to be more critical in the root even though these gene transcripts had a higher induction in the shoot. In the phr1 shoot, the genes were induced by more or less half the wild-type response (Fig. 5, Supplementary Table S3 at JXB online), while in the root the induction of most genes was practically abolished. Only four gene transcripts (PLDZ2, PLA2A, MGD3, and SQD1) were still found to be slightly induced in phr1 roots, none of them >2-fold Fig. 5, Supplementary Table S3 at JXB online.

Bottom Line: It consists of the degradation of phospholipids to release the phosphate needed by the cell and the accumulation of glycolipids to replace phospholipids in the membranes.Triacylglycerol (TAG) accumulates dramatically under P starvation (by as much as ~20-fold in shoots and ~13-fold in roots), a response known to occur in green algae but hardly known in plants.Surprisingly, there was an increase in phosphatidylglycerol (PG) in P-starved roots, a response that may be adaptive as it was suppressed in the phr1 mutant.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA.

No MeSH data available.


Related in: MedlinePlus