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Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism.

Falcinelli S, Picchietti S, Rodiles A, Cossignani L, Merrifield DL, Taddei AR, Maradonna F, Olivotto I, Gioacchini G, Carnevali O - Sci Rep (2015)

Bottom Line: Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria.L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium.These changes resulted in elevated zebrafish larval growth.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.

ABSTRACT
The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (fit2, agpat4, dgat2, mgll, hnf4α, scap, and cck) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism, and reveals a new potential role for L. rhamnosus in the treatment of lipid disorders.

No MeSH data available.


Related in: MedlinePlus

L. rhamnosus administration increased larvae total length and body weight.Total length (A) and wet weight (B) of zebrafish larvae from control and probiotic groups collected at 96 hpf, 6 dpf and 8 dpf. Data are the mean ± s.d. Asterisk indicates significant differences (P < 0.05).
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f5: L. rhamnosus administration increased larvae total length and body weight.Total length (A) and wet weight (B) of zebrafish larvae from control and probiotic groups collected at 96 hpf, 6 dpf and 8 dpf. Data are the mean ± s.d. Asterisk indicates significant differences (P < 0.05).

Mentions: Since we reported compelling evidence of gut community modulation and in turn lipid metabolism modulation after administration of L. rhamnosus, by measuring total larval length and wet weight, we sought to determine if these changes impacted the growth of zebrafish larvae. At 96 hpf no significance differences were observed in terms of length and weight in both control (length 3.3 ± 0.17 mm; weight 0.43 ± 0.07 mg) and treated (length 3.4 ± 0.11 mm, weight 0.4 ± 0.08 mg) larvae (P > 0.05). At 6 dpf no significant difference of larval length was observed between control larvae (3.5 ± 0.16 mm) and larvae exposed to L. rhamnosus (3.6 ± 0.18 mm) (P > 0.05). Despite this, a significant increase of wet weight was observed at 6 dpf in the probiotic supplemented group (1.19 ± 0.09 mg) compared to the control group (0.9 ± 0.1 mg). At 8 dpf a significant increase of total length (3.9 ± 0.12 mm) concomitant with a significant increase of wet weight (1.37 ± 0.13 mg) was apparent in zebrafish larvae treated with the probiotic compared to the length (3.6 ± 0.21 mm) and wet weight (1.07 ± 0.08 mg) detected in control group (P < 0.05) (Figure 5A–B). In all stages examined the viable larvae observed in both control and treated groups were 95–98%.


Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism.

Falcinelli S, Picchietti S, Rodiles A, Cossignani L, Merrifield DL, Taddei AR, Maradonna F, Olivotto I, Gioacchini G, Carnevali O - Sci Rep (2015)

L. rhamnosus administration increased larvae total length and body weight.Total length (A) and wet weight (B) of zebrafish larvae from control and probiotic groups collected at 96 hpf, 6 dpf and 8 dpf. Data are the mean ± s.d. Asterisk indicates significant differences (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4378510&req=5

f5: L. rhamnosus administration increased larvae total length and body weight.Total length (A) and wet weight (B) of zebrafish larvae from control and probiotic groups collected at 96 hpf, 6 dpf and 8 dpf. Data are the mean ± s.d. Asterisk indicates significant differences (P < 0.05).
Mentions: Since we reported compelling evidence of gut community modulation and in turn lipid metabolism modulation after administration of L. rhamnosus, by measuring total larval length and wet weight, we sought to determine if these changes impacted the growth of zebrafish larvae. At 96 hpf no significance differences were observed in terms of length and weight in both control (length 3.3 ± 0.17 mm; weight 0.43 ± 0.07 mg) and treated (length 3.4 ± 0.11 mm, weight 0.4 ± 0.08 mg) larvae (P > 0.05). At 6 dpf no significant difference of larval length was observed between control larvae (3.5 ± 0.16 mm) and larvae exposed to L. rhamnosus (3.6 ± 0.18 mm) (P > 0.05). Despite this, a significant increase of wet weight was observed at 6 dpf in the probiotic supplemented group (1.19 ± 0.09 mg) compared to the control group (0.9 ± 0.1 mg). At 8 dpf a significant increase of total length (3.9 ± 0.12 mm) concomitant with a significant increase of wet weight (1.37 ± 0.13 mg) was apparent in zebrafish larvae treated with the probiotic compared to the length (3.6 ± 0.21 mm) and wet weight (1.07 ± 0.08 mg) detected in control group (P < 0.05) (Figure 5A–B). In all stages examined the viable larvae observed in both control and treated groups were 95–98%.

Bottom Line: Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria.L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium.These changes resulted in elevated zebrafish larval growth.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.

ABSTRACT
The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (fit2, agpat4, dgat2, mgll, hnf4α, scap, and cck) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism, and reveals a new potential role for L. rhamnosus in the treatment of lipid disorders.

No MeSH data available.


Related in: MedlinePlus