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Pathogenicity of 2 porcine deltacoronavirus strains in gnotobiotic pigs.

Jung K, Hu H, Eyerly B, Lu Z, Chepngeno J, Saif LJ - Emerging Infect. Dis. (2015)

Bottom Line: To verify whether porcine deltacoronavirus infection induces disease, we inoculated gnotobiotic pigs with 2 virus strains (OH-FD22 and OH-FD100) identified by 2 specific reverse transcription PCRs.At 21-120 h postinoculation, pigs exhibited severe diarrhea, vomiting, fecal shedding of virus, and severe atrophic enteritis.These findings confirm that these 2 strains are enteropathogenic in pigs.

View Article: PubMed Central - PubMed

ABSTRACT
To verify whether porcine deltacoronavirus infection induces disease, we inoculated gnotobiotic pigs with 2 virus strains (OH-FD22 and OH-FD100) identified by 2 specific reverse transcription PCRs. At 21-120 h postinoculation, pigs exhibited severe diarrhea, vomiting, fecal shedding of virus, and severe atrophic enteritis. These findings confirm that these 2 strains are enteropathogenic in pigs.

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Related in: MedlinePlus

Porcine deltacoronavirus (OH-FD22) particle detected in intestinal contents from a gnotobiotic pig. The sample was negatively stained with 3% phosphotungstic acid. Scale bar indicates 100 nm.
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Figure 1: Porcine deltacoronavirus (OH-FD22) particle detected in intestinal contents from a gnotobiotic pig. The sample was negatively stained with 3% phosphotungstic acid. Scale bar indicates 100 nm.

Mentions: Acute, severe, watery diarrhea, vomiting, or both developed in all inoculated pigs. Clinical signs developed at hour postinoculation (hpi) 21–24, regardless of the inoculum strain or dose (Table 1). At hpi 96–120, pig 1 exhibited severe dehydration, loss of bodyweight, and lethargy. Pig 2, which was followed up longer, showed diarrhea until dpi 7. All inoculated pigs exhibited onset of clinical disease similar to that of infection with PEDV strain PC21A (6.3–9.0 log10 GEs/pig) in gnotobiotic pigs (8). Immune electron microscopy with hyperimmune serum to PDCoV from a gnotobiotic pig showed only PDCoV particles in the intestinal contents (Figure 1). For pig-passaged OH-FD22 and OH-FD100 samples, RT-PCR results were negative for PEDV, rotavirus groups A–C, TGEV/PRCV, and caliciviruses. Detection of fecal virus shedding at hpi 24 coincided with onset of clinical signs (Table 1) in pigs 2–5. In pig 1, which showed only vomiting at hpi 24, fecal shedding occurred at hpi 48 at the onset of diarrhea.


Pathogenicity of 2 porcine deltacoronavirus strains in gnotobiotic pigs.

Jung K, Hu H, Eyerly B, Lu Z, Chepngeno J, Saif LJ - Emerging Infect. Dis. (2015)

Porcine deltacoronavirus (OH-FD22) particle detected in intestinal contents from a gnotobiotic pig. The sample was negatively stained with 3% phosphotungstic acid. Scale bar indicates 100 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4378491&req=5

Figure 1: Porcine deltacoronavirus (OH-FD22) particle detected in intestinal contents from a gnotobiotic pig. The sample was negatively stained with 3% phosphotungstic acid. Scale bar indicates 100 nm.
Mentions: Acute, severe, watery diarrhea, vomiting, or both developed in all inoculated pigs. Clinical signs developed at hour postinoculation (hpi) 21–24, regardless of the inoculum strain or dose (Table 1). At hpi 96–120, pig 1 exhibited severe dehydration, loss of bodyweight, and lethargy. Pig 2, which was followed up longer, showed diarrhea until dpi 7. All inoculated pigs exhibited onset of clinical disease similar to that of infection with PEDV strain PC21A (6.3–9.0 log10 GEs/pig) in gnotobiotic pigs (8). Immune electron microscopy with hyperimmune serum to PDCoV from a gnotobiotic pig showed only PDCoV particles in the intestinal contents (Figure 1). For pig-passaged OH-FD22 and OH-FD100 samples, RT-PCR results were negative for PEDV, rotavirus groups A–C, TGEV/PRCV, and caliciviruses. Detection of fecal virus shedding at hpi 24 coincided with onset of clinical signs (Table 1) in pigs 2–5. In pig 1, which showed only vomiting at hpi 24, fecal shedding occurred at hpi 48 at the onset of diarrhea.

Bottom Line: To verify whether porcine deltacoronavirus infection induces disease, we inoculated gnotobiotic pigs with 2 virus strains (OH-FD22 and OH-FD100) identified by 2 specific reverse transcription PCRs.At 21-120 h postinoculation, pigs exhibited severe diarrhea, vomiting, fecal shedding of virus, and severe atrophic enteritis.These findings confirm that these 2 strains are enteropathogenic in pigs.

View Article: PubMed Central - PubMed

ABSTRACT
To verify whether porcine deltacoronavirus infection induces disease, we inoculated gnotobiotic pigs with 2 virus strains (OH-FD22 and OH-FD100) identified by 2 specific reverse transcription PCRs. At 21-120 h postinoculation, pigs exhibited severe diarrhea, vomiting, fecal shedding of virus, and severe atrophic enteritis. These findings confirm that these 2 strains are enteropathogenic in pigs.

Show MeSH
Related in: MedlinePlus