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Candidate new rotavirus species in sheltered dogs, Hungary.

Mihalov-Kovács E, Gellért Á, Marton S, Farkas SL, Fehér E, Oldal M, Jakab F, Martella V, Bányai K - Emerging Infect. Dis. (2015)

Bottom Line: We identified unusual rotavirus strains in fecal specimens from sheltered dogs in Hungary by viral metagenomics.The novel rotavirus species displayed limited genome sequence homology to representatives of the 8 rotavirus species, A-H, and qualifies as a candidate new rotavirus species that we tentatively named Rotavirus I.

View Article: PubMed Central - PubMed

ABSTRACT
We identified unusual rotavirus strains in fecal specimens from sheltered dogs in Hungary by viral metagenomics. The novel rotavirus species displayed limited genome sequence homology to representatives of the 8 rotavirus species, A-H, and qualifies as a candidate new rotavirus species that we tentatively named Rotavirus I.

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Structure comparison of rotavirus viral protein (VP) 6 proteins. A) Structure-based amino acid sequence alignment of the novel canine rotavirus VP6 protein and the template bovine rotavirus A VP6 protein. The background of the sequence alignments reflects the homology levels of the 2 VP6 sequences. Red, identical amino acid; orange, similar amino acid; pink, different amino acid). The main structural differences are indicated by dark red and menthol green on the sequence alignment and on the superimposed VP6 structures (B). Cartoon presentation of the homologous VP6 proteins: pink, rotavirus A; green, rotavirus I. Further information is available in the Technical Appendix.
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Figure 1: Structure comparison of rotavirus viral protein (VP) 6 proteins. A) Structure-based amino acid sequence alignment of the novel canine rotavirus VP6 protein and the template bovine rotavirus A VP6 protein. The background of the sequence alignments reflects the homology levels of the 2 VP6 sequences. Red, identical amino acid; orange, similar amino acid; pink, different amino acid). The main structural differences are indicated by dark red and menthol green on the sequence alignment and on the superimposed VP6 structures (B). Cartoon presentation of the homologous VP6 proteins: pink, rotavirus A; green, rotavirus I. Further information is available in the Technical Appendix.

Mentions: To extend the analysis and assess whether the obtained VP6 gene might be functionally integral, we conducted molecular modeling of the amino acid sequence. In brief, amino acid sequence similarity values created a reliable protein model (8,9) showing similar protein folding of the VP6 monomer and comparable electrostatic charge pattern around the 3-fold axis of the VP6 homotrimer to that experimentally determined for rotavirus A (Figure 1). Subsequent phylogenetic analysis of the VP6 protein identified 2 major clades of rotaviruses (6). The novel rotavirus strains clustered with species H, G, and B within clade 2, whereas clade 1 included representative strains of species A, C, D, and F (Figure 2). This pattern of clustering was also evident when we analyzed the remaining genes. Collectively, sequence and phylogenetic analysis demonstrated moderate genetic relatedness of the unusual canine rotaviruses to representative strains of species A–H, suggesting that they belong to a novel species, tentatively called Rotavirus I. The prototype strains were named RVI/Dog-wt/HUN/KE135/2012/G1P1 and RVI/Dog-wt/HUN/KE528/2012/G2P1 according to recent guidelines (10) (online Technical Appendix).


Candidate new rotavirus species in sheltered dogs, Hungary.

Mihalov-Kovács E, Gellért Á, Marton S, Farkas SL, Fehér E, Oldal M, Jakab F, Martella V, Bányai K - Emerging Infect. Dis. (2015)

Structure comparison of rotavirus viral protein (VP) 6 proteins. A) Structure-based amino acid sequence alignment of the novel canine rotavirus VP6 protein and the template bovine rotavirus A VP6 protein. The background of the sequence alignments reflects the homology levels of the 2 VP6 sequences. Red, identical amino acid; orange, similar amino acid; pink, different amino acid). The main structural differences are indicated by dark red and menthol green on the sequence alignment and on the superimposed VP6 structures (B). Cartoon presentation of the homologous VP6 proteins: pink, rotavirus A; green, rotavirus I. Further information is available in the Technical Appendix.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4378476&req=5

Figure 1: Structure comparison of rotavirus viral protein (VP) 6 proteins. A) Structure-based amino acid sequence alignment of the novel canine rotavirus VP6 protein and the template bovine rotavirus A VP6 protein. The background of the sequence alignments reflects the homology levels of the 2 VP6 sequences. Red, identical amino acid; orange, similar amino acid; pink, different amino acid). The main structural differences are indicated by dark red and menthol green on the sequence alignment and on the superimposed VP6 structures (B). Cartoon presentation of the homologous VP6 proteins: pink, rotavirus A; green, rotavirus I. Further information is available in the Technical Appendix.
Mentions: To extend the analysis and assess whether the obtained VP6 gene might be functionally integral, we conducted molecular modeling of the amino acid sequence. In brief, amino acid sequence similarity values created a reliable protein model (8,9) showing similar protein folding of the VP6 monomer and comparable electrostatic charge pattern around the 3-fold axis of the VP6 homotrimer to that experimentally determined for rotavirus A (Figure 1). Subsequent phylogenetic analysis of the VP6 protein identified 2 major clades of rotaviruses (6). The novel rotavirus strains clustered with species H, G, and B within clade 2, whereas clade 1 included representative strains of species A, C, D, and F (Figure 2). This pattern of clustering was also evident when we analyzed the remaining genes. Collectively, sequence and phylogenetic analysis demonstrated moderate genetic relatedness of the unusual canine rotaviruses to representative strains of species A–H, suggesting that they belong to a novel species, tentatively called Rotavirus I. The prototype strains were named RVI/Dog-wt/HUN/KE135/2012/G1P1 and RVI/Dog-wt/HUN/KE528/2012/G2P1 according to recent guidelines (10) (online Technical Appendix).

Bottom Line: We identified unusual rotavirus strains in fecal specimens from sheltered dogs in Hungary by viral metagenomics.The novel rotavirus species displayed limited genome sequence homology to representatives of the 8 rotavirus species, A-H, and qualifies as a candidate new rotavirus species that we tentatively named Rotavirus I.

View Article: PubMed Central - PubMed

ABSTRACT
We identified unusual rotavirus strains in fecal specimens from sheltered dogs in Hungary by viral metagenomics. The novel rotavirus species displayed limited genome sequence homology to representatives of the 8 rotavirus species, A-H, and qualifies as a candidate new rotavirus species that we tentatively named Rotavirus I.

Show MeSH