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Chemical methods for decoding cytosine modifications in DNA.

Booth MJ, Raiber EA, Balasubramanian S - Chem. Rev. (2014)

View Article: PubMed Central - PubMed

Affiliation: †Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW United Kingdom.

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and therefore does not introduce a strand cleavage... This results in hydroysis with loss of ammonia... The resulting uracil bisulfite adduct also decreases at pH values below 5.0 due to the protonation of the most effective catalytic species, sulphite... with 5mC than with C, due to inhibition of the adduct formation from the electronic effect of the methyl group... buffers”; however, there has been no definitive examination of their effectiveness... resolution map of 5mC, 5hmC and 5fCs... 5hmC and, through oxidiation of 5mC with the TET enzymes, have been might be feasible to use such nanopores to discriminate 5mC and 5hmC By chemically altering the primary alcohol of 5hmC it is It is now possible to decode 5mC, 5hmC,

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Potential pathways for DNA demethylation.
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fig4: Potential pathways for DNA demethylation.

Mentions: There are several proposedpathways for demethylation; one mechanismsuggests the iterative oxidation of 5mC by the TET family enzymes,followed by base excision repair or deformylation/decarboxylation.A potential mechanism for active demethylation is through the thymineDNA glycosylase (TDG) enzyme, which can excise both 5fC and 5caC fromDNA but does not remove 5mC or 5hmC.13c,17 Followingthis base excision the abasic site would be repaired by the base excisionrepair (BER) pathway.18 It is also possiblethat a decarboxylase enzyme could directly remove the carboxylic acidgroup from 5caC (Figure 4).19


Chemical methods for decoding cytosine modifications in DNA.

Booth MJ, Raiber EA, Balasubramanian S - Chem. Rev. (2014)

Potential pathways for DNA demethylation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4378238&req=5

fig4: Potential pathways for DNA demethylation.
Mentions: There are several proposedpathways for demethylation; one mechanismsuggests the iterative oxidation of 5mC by the TET family enzymes,followed by base excision repair or deformylation/decarboxylation.A potential mechanism for active demethylation is through the thymineDNA glycosylase (TDG) enzyme, which can excise both 5fC and 5caC fromDNA but does not remove 5mC or 5hmC.13c,17 Followingthis base excision the abasic site would be repaired by the base excisionrepair (BER) pathway.18 It is also possiblethat a decarboxylase enzyme could directly remove the carboxylic acidgroup from 5caC (Figure 4).19

View Article: PubMed Central - PubMed

Affiliation: †Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW United Kingdom.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

and therefore does not introduce a strand cleavage... This results in hydroysis with loss of ammonia... The resulting uracil bisulfite adduct also decreases at pH values below 5.0 due to the protonation of the most effective catalytic species, sulphite... with 5mC than with C, due to inhibition of the adduct formation from the electronic effect of the methyl group... buffers”; however, there has been no definitive examination of their effectiveness... resolution map of 5mC, 5hmC and 5fCs... 5hmC and, through oxidiation of 5mC with the TET enzymes, have been might be feasible to use such nanopores to discriminate 5mC and 5hmC By chemically altering the primary alcohol of 5hmC it is It is now possible to decode 5mC, 5hmC,

Show MeSH