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Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil.

Pereira Júnior AM, Teles CB, de Azevedo dos Santos AP, de Souza Rodrigues M, Marialva EF, Pessoa FA, Medeiros JF - Parasit Vectors (2015)

Bottom Line: Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species).DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies.

View Article: PubMed Central - PubMed

Affiliation: Instituto Nacional de Pesquisas da Amazônia/Fiocruz Rondônia, Porto Velho, Rondônia, Brazil. junior.ampj@gmail.com.

ABSTRACT

Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations.

Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing.

Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.

Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA.

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Related in: MedlinePlus

Abundance curves. Sand flies species in terra firme and várzea environments, captured in the months of January, February, April, August, September and October of 2013, in Tefé municipality, Amazonas State, Brazil.
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Fig2: Abundance curves. Sand flies species in terra firme and várzea environments, captured in the months of January, February, April, August, September and October of 2013, in Tefé municipality, Amazonas State, Brazil.

Mentions: In all, 5428 specimens were collected from terra firme environments, comprising 42 species and 6 morphotypes. The most abundant species were Thichophoromyia ubiquitalis (3312 individuals – 57.94%), Nyssomyia antunesi (526 – 9.20%), Nyssomyia yuilli yuilli (259 – 4.53%), Trichophoromyia sp. (207 – 3.62%), Psychodopygus davisi (198 – 3.46%), and Trichophoromyia melloi (146 - 2.55%). In the várzea environment, 288 specimens were collected, comprising 22 species and 3 morphotypes. Of these, the most abundant species were Ny. antunesi (135 - 2.36%) and Evandromyia walkeri (87 – 1.52%) (Table 1). Both environments had high species richness, but equitability was low (Figure 2). Analysis by IndVal indicated that only four species were specific to the terra firme environment: Th. ubiquitalis, Ps. davisi, Ny. yuilli yuilli, and Th. melloi (Table 2). No species were specific to the várzea environment, but Evandromyia walkeri was found there in higher frequency.Figure 2


Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil.

Pereira Júnior AM, Teles CB, de Azevedo dos Santos AP, de Souza Rodrigues M, Marialva EF, Pessoa FA, Medeiros JF - Parasit Vectors (2015)

Abundance curves. Sand flies species in terra firme and várzea environments, captured in the months of January, February, April, August, September and October of 2013, in Tefé municipality, Amazonas State, Brazil.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4378226&req=5

Fig2: Abundance curves. Sand flies species in terra firme and várzea environments, captured in the months of January, February, April, August, September and October of 2013, in Tefé municipality, Amazonas State, Brazil.
Mentions: In all, 5428 specimens were collected from terra firme environments, comprising 42 species and 6 morphotypes. The most abundant species were Thichophoromyia ubiquitalis (3312 individuals – 57.94%), Nyssomyia antunesi (526 – 9.20%), Nyssomyia yuilli yuilli (259 – 4.53%), Trichophoromyia sp. (207 – 3.62%), Psychodopygus davisi (198 – 3.46%), and Trichophoromyia melloi (146 - 2.55%). In the várzea environment, 288 specimens were collected, comprising 22 species and 3 morphotypes. Of these, the most abundant species were Ny. antunesi (135 - 2.36%) and Evandromyia walkeri (87 – 1.52%) (Table 1). Both environments had high species richness, but equitability was low (Figure 2). Analysis by IndVal indicated that only four species were specific to the terra firme environment: Th. ubiquitalis, Ps. davisi, Ny. yuilli yuilli, and Th. melloi (Table 2). No species were specific to the várzea environment, but Evandromyia walkeri was found there in higher frequency.Figure 2

Bottom Line: Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species).DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies.

View Article: PubMed Central - PubMed

Affiliation: Instituto Nacional de Pesquisas da Amazônia/Fiocruz Rondônia, Porto Velho, Rondônia, Brazil. junior.ampj@gmail.com.

ABSTRACT

Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations.

Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing.

Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.

Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA.

Show MeSH
Related in: MedlinePlus