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Muc5ac mucin expression during rat skin development.

Ferretti V, Segal-Eiras Á, Barbeito CG, Croce MV - Eur J Histochem (2015)

Bottom Line: Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species.By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells.To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

View Article: PubMed Central - PubMed

Affiliation: National University of La Plata. crocevir@hotmail.com.

ABSTRACT

Unlabelled: Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

Conclusion:   Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

No MeSH data available.


Related in: MedlinePlus

Expression of Muc1 determinates using immunohistochemistry in rat skin samples of embryos and adults. A) Epidermis at 20 days of gestation showing a negative reaction. B) Adult skin; the reaction was restricted to the sebaceous glands. C) Magnification of the box in panel B. D) Sebaceous glands of adult skin showing a high Muc1 expression.
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fig004: Expression of Muc1 determinates using immunohistochemistry in rat skin samples of embryos and adults. A) Epidermis at 20 days of gestation showing a negative reaction. B) Adult skin; the reaction was restricted to the sebaceous glands. C) Magnification of the box in panel B. D) Sebaceous glands of adult skin showing a high Muc1 expression.

Mentions: By IHC, Muc5ac protein was found early in embryonic epidermis, at day 13 of gestation (D13), with a moderate intensity and a predominantly cytoplasmic pattern (Table 1) (Figure 1A); from D16, the intensity of the reaction increased (Figure 1 B-F). Muc5ac expression was further analyzed in rat neonatal skin samples at different days of development from 2 to 15 days after birth (post-natal development, PD); in samples obtained between 2 and 6PD, a high intensity was detected at different layers of the epidermis. In addition, secretory cells of the sebaceous glands associated with hair follicles showed Muc5ac expression (Figure 2A). At 7PD, the surface epidermis became negative and the reaction was restricted to secreting sebum cells (Figure 2B) which showed a strong cytoplasmic reaction; these results are in accordance with those obtained on adult rat samples (Figure 2 C,D). Expression of cytokeratin 5/6 (ck5/6) and Muc1 was also analyzed in rat skin samples of embryos, neonates and adults at different days of development. Both Ck5/6 and Muc1 were negative in embryonic epidermis (Figure 3 A-C; Figure 4A). However, both antigens were restricted to secreting sebum cells and in some cases to basal keratinocytes in neonatal and adult skin samples (Figure 3D; Figure 4 B-D). The reaction was low in neonates and moderate in adults.


Muc5ac mucin expression during rat skin development.

Ferretti V, Segal-Eiras Á, Barbeito CG, Croce MV - Eur J Histochem (2015)

Expression of Muc1 determinates using immunohistochemistry in rat skin samples of embryos and adults. A) Epidermis at 20 days of gestation showing a negative reaction. B) Adult skin; the reaction was restricted to the sebaceous glands. C) Magnification of the box in panel B. D) Sebaceous glands of adult skin showing a high Muc1 expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4378216&req=5

fig004: Expression of Muc1 determinates using immunohistochemistry in rat skin samples of embryos and adults. A) Epidermis at 20 days of gestation showing a negative reaction. B) Adult skin; the reaction was restricted to the sebaceous glands. C) Magnification of the box in panel B. D) Sebaceous glands of adult skin showing a high Muc1 expression.
Mentions: By IHC, Muc5ac protein was found early in embryonic epidermis, at day 13 of gestation (D13), with a moderate intensity and a predominantly cytoplasmic pattern (Table 1) (Figure 1A); from D16, the intensity of the reaction increased (Figure 1 B-F). Muc5ac expression was further analyzed in rat neonatal skin samples at different days of development from 2 to 15 days after birth (post-natal development, PD); in samples obtained between 2 and 6PD, a high intensity was detected at different layers of the epidermis. In addition, secretory cells of the sebaceous glands associated with hair follicles showed Muc5ac expression (Figure 2A). At 7PD, the surface epidermis became negative and the reaction was restricted to secreting sebum cells (Figure 2B) which showed a strong cytoplasmic reaction; these results are in accordance with those obtained on adult rat samples (Figure 2 C,D). Expression of cytokeratin 5/6 (ck5/6) and Muc1 was also analyzed in rat skin samples of embryos, neonates and adults at different days of development. Both Ck5/6 and Muc1 were negative in embryonic epidermis (Figure 3 A-C; Figure 4A). However, both antigens were restricted to secreting sebum cells and in some cases to basal keratinocytes in neonatal and adult skin samples (Figure 3D; Figure 4 B-D). The reaction was low in neonates and moderate in adults.

Bottom Line: Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species.By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells.To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

View Article: PubMed Central - PubMed

Affiliation: National University of La Plata. crocevir@hotmail.com.

ABSTRACT

Unlabelled: Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

Conclusion:   Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.

No MeSH data available.


Related in: MedlinePlus