Limits...
Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B Pathway.

Zhang XA, Zhang S, Yin Q, Zhang J - Pharmacogn Mag (2015 Apr-Jun)

Bottom Line: Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis as well as the antioxidant functions.However, it is not fully elucidated in colon cancer.In this study, we demonstrate that quercetin induces apoptosis in human colon cancer CACO-2 and SW-620 cells through inhibiting NF-κB pathway, as well as down-regulation of B-cell lymphoma 2 and up-regulation of Bax, thus providing basis for clinical application of quercetin in colon cancer cases.

View Article: PubMed Central - PubMed

Affiliation: Anorectal Disease Center, The First Affiliated Hospital, Henan College of TCM, Zhengzhou 450000, China.

ABSTRACT
Quercetin can inhibit the growth of cancer cells with the ability to act as chemopreventers. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis as well as the antioxidant functions. Nuclear factor kappa-B (NF-κB) is a signaling pathway that controls transcriptional activation of genes important for tight regulation of many cellular processes and is aberrantly expressed in many types of cancer. Inhibitors of NF-κB pathway have shown potential anti-tumor activities. However, it is not fully elucidated in colon cancer. In this study, we demonstrate that quercetin induces apoptosis in human colon cancer CACO-2 and SW-620 cells through inhibiting NF-κB pathway, as well as down-regulation of B-cell lymphoma 2 and up-regulation of Bax, thus providing basis for clinical application of quercetin in colon cancer cases.

No MeSH data available.


Related in: MedlinePlus

Protein expression of cleaved poly (ADP-ribose) polymerase and apoptosis inducing factor in CACO-2 and SW-620 cells after indicated treatments was measured by western blots. Glyceraldehyde 3-phosphate dehydrogenase served as a control for loading
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4378141&req=5

Figure 6: Protein expression of cleaved poly (ADP-ribose) polymerase and apoptosis inducing factor in CACO-2 and SW-620 cells after indicated treatments was measured by western blots. Glyceraldehyde 3-phosphate dehydrogenase served as a control for loading

Mentions: In order to gain a better insight into pro-apoptotic effect of quercetin, we detected protein expression of apoptosis marker molecular. Poly (ADP-ribose) polymerase (PARP) was one of the main cleavage targets of caspase-3 and cleaved PARP always served as a marker of cells undergoing apoptosis.[33] Results demonstrated that cleaved PARP could not be detected until quercetin treated was administrated at the high dose of 30 μM, further suggesting that quercetin could induce apoptosis in a dose-dependent manner [Figure 6]. We also measured the expression of apoptosis inducing factor (AIF), which played a critical role in caspase-independent apoptosis.[34] However, results demonstrated that no increase in AIF expression was detected after quercetin treatment [Figure 6].


Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B Pathway.

Zhang XA, Zhang S, Yin Q, Zhang J - Pharmacogn Mag (2015 Apr-Jun)

Protein expression of cleaved poly (ADP-ribose) polymerase and apoptosis inducing factor in CACO-2 and SW-620 cells after indicated treatments was measured by western blots. Glyceraldehyde 3-phosphate dehydrogenase served as a control for loading
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4378141&req=5

Figure 6: Protein expression of cleaved poly (ADP-ribose) polymerase and apoptosis inducing factor in CACO-2 and SW-620 cells after indicated treatments was measured by western blots. Glyceraldehyde 3-phosphate dehydrogenase served as a control for loading
Mentions: In order to gain a better insight into pro-apoptotic effect of quercetin, we detected protein expression of apoptosis marker molecular. Poly (ADP-ribose) polymerase (PARP) was one of the main cleavage targets of caspase-3 and cleaved PARP always served as a marker of cells undergoing apoptosis.[33] Results demonstrated that cleaved PARP could not be detected until quercetin treated was administrated at the high dose of 30 μM, further suggesting that quercetin could induce apoptosis in a dose-dependent manner [Figure 6]. We also measured the expression of apoptosis inducing factor (AIF), which played a critical role in caspase-independent apoptosis.[34] However, results demonstrated that no increase in AIF expression was detected after quercetin treatment [Figure 6].

Bottom Line: Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis as well as the antioxidant functions.However, it is not fully elucidated in colon cancer.In this study, we demonstrate that quercetin induces apoptosis in human colon cancer CACO-2 and SW-620 cells through inhibiting NF-κB pathway, as well as down-regulation of B-cell lymphoma 2 and up-regulation of Bax, thus providing basis for clinical application of quercetin in colon cancer cases.

View Article: PubMed Central - PubMed

Affiliation: Anorectal Disease Center, The First Affiliated Hospital, Henan College of TCM, Zhengzhou 450000, China.

ABSTRACT
Quercetin can inhibit the growth of cancer cells with the ability to act as chemopreventers. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis as well as the antioxidant functions. Nuclear factor kappa-B (NF-κB) is a signaling pathway that controls transcriptional activation of genes important for tight regulation of many cellular processes and is aberrantly expressed in many types of cancer. Inhibitors of NF-κB pathway have shown potential anti-tumor activities. However, it is not fully elucidated in colon cancer. In this study, we demonstrate that quercetin induces apoptosis in human colon cancer CACO-2 and SW-620 cells through inhibiting NF-κB pathway, as well as down-regulation of B-cell lymphoma 2 and up-regulation of Bax, thus providing basis for clinical application of quercetin in colon cancer cases.

No MeSH data available.


Related in: MedlinePlus