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The marine metabolite SZ-685C induces apoptosis in primary human nonfunctioning pituitary adenoma cells by inhibition of the Akt pathway in vitro.

Wang X, Tan T, Mao ZG, Lei N, Wang ZM, Hu B, Chen ZY, She ZG, Zhu YH, Wang HJ - Mar Drugs (2015)

Bottom Line: The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea.Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects.Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Medical school of Sun Yat-sen University, No.74, Zhongshan Road 2, Guangzhou 510080, China. wangx357@mail2.sysu.edu.cn.

ABSTRACT
Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects. The tetrazolium-based colorimetric assay (MTT assay) to investigate the different effect of the marine compound SZ-685C on the proliferation of primary human NFPA cells, rat normal pituitary cells (RPCs) and rat prolactinoma MMQ cell lines. Hoechst 33342 dye/propidium iodide (PI) double staining and fluorescein isothiocyanate-conjugated Annexin V/PI (Annexin V-FITC/PI) apoptosis assays detected an enhanced rate of apoptosis in cells treated with SZ-685C. Enhanced expression levels of caspase 3 and phosphate and tensin homolog (PTEN) were determined by Western blotting. Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C. Here, we show that SZ-685C induces apoptosis of human NFPA cells through inhibition of the Akt pathway in vitro. The understanding of apoptosis has provided the basis for novel targeted therapies that can induce death in cancer cells or sensitize them to established cytotoxic agents and radiation therapy.

No MeSH data available.


Related in: MedlinePlus

(A) CK8 and Hoechst immunofluorescent staining; (B) Vimentin and Hoechst immunofluorescent staining. Magnification: × 400, scale bar: 100 μm.
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marinedrugs-13-01569-f003: (A) CK8 and Hoechst immunofluorescent staining; (B) Vimentin and Hoechst immunofluorescent staining. Magnification: × 400, scale bar: 100 μm.

Mentions: Cytokeratins (CKs) are typically expressed in epithelial cells, whereas vimentin can be found in cells of mesenchymal origin. Co-expression of vimentin and CKs is believed to be the hallmark of epithelial-to-mesenchymal or mesenchymal-to-epithelial transformations of developing tissues. In this study, we analyzed the expression and co-expression of simple epithelial CK8 and vimentin in NFPA cells. The cells’ nuclei were stained with Hoechst 33342 dye, which emits a blue fluorescence, and CK8 and vimentin, which emit a green fluorescence (Figure 3).


The marine metabolite SZ-685C induces apoptosis in primary human nonfunctioning pituitary adenoma cells by inhibition of the Akt pathway in vitro.

Wang X, Tan T, Mao ZG, Lei N, Wang ZM, Hu B, Chen ZY, She ZG, Zhu YH, Wang HJ - Mar Drugs (2015)

(A) CK8 and Hoechst immunofluorescent staining; (B) Vimentin and Hoechst immunofluorescent staining. Magnification: × 400, scale bar: 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4378000&req=5

marinedrugs-13-01569-f003: (A) CK8 and Hoechst immunofluorescent staining; (B) Vimentin and Hoechst immunofluorescent staining. Magnification: × 400, scale bar: 100 μm.
Mentions: Cytokeratins (CKs) are typically expressed in epithelial cells, whereas vimentin can be found in cells of mesenchymal origin. Co-expression of vimentin and CKs is believed to be the hallmark of epithelial-to-mesenchymal or mesenchymal-to-epithelial transformations of developing tissues. In this study, we analyzed the expression and co-expression of simple epithelial CK8 and vimentin in NFPA cells. The cells’ nuclei were stained with Hoechst 33342 dye, which emits a blue fluorescence, and CK8 and vimentin, which emit a green fluorescence (Figure 3).

Bottom Line: The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea.Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects.Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Medical school of Sun Yat-sen University, No.74, Zhongshan Road 2, Guangzhou 510080, China. wangx357@mail2.sysu.edu.cn.

ABSTRACT
Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects. The tetrazolium-based colorimetric assay (MTT assay) to investigate the different effect of the marine compound SZ-685C on the proliferation of primary human NFPA cells, rat normal pituitary cells (RPCs) and rat prolactinoma MMQ cell lines. Hoechst 33342 dye/propidium iodide (PI) double staining and fluorescein isothiocyanate-conjugated Annexin V/PI (Annexin V-FITC/PI) apoptosis assays detected an enhanced rate of apoptosis in cells treated with SZ-685C. Enhanced expression levels of caspase 3 and phosphate and tensin homolog (PTEN) were determined by Western blotting. Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C. Here, we show that SZ-685C induces apoptosis of human NFPA cells through inhibition of the Akt pathway in vitro. The understanding of apoptosis has provided the basis for novel targeted therapies that can induce death in cancer cells or sensitize them to established cytotoxic agents and radiation therapy.

No MeSH data available.


Related in: MedlinePlus