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Structural analysis and anti-complement activity of polysaccharides from Kjellmaniella crsaaifolia.

Zhang W, Jin W, Sun D, Zhao L, Wang J, Duan D, Zhang Q - Mar Drugs (2015)

Bottom Line: The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity.In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity.In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wenjingwing@126.com.

ABSTRACT
Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.

No MeSH data available.


Related in: MedlinePlus

Inhibition of the classical pathway-mediated hemolysis of EA (a and b) and alternative pathway-mediated hemolysis of ER (c and d) in 1:10-diluted NHS in the presence of increasing amounts of the polysaccharides. Heparin was used as the reference. The results are expressed as percent inhibition of hemolysis. Data are the means from 3 determinations ± S.E.M.
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marinedrugs-13-01360-f007: Inhibition of the classical pathway-mediated hemolysis of EA (a and b) and alternative pathway-mediated hemolysis of ER (c and d) in 1:10-diluted NHS in the presence of increasing amounts of the polysaccharides. Heparin was used as the reference. The results are expressed as percent inhibition of hemolysis. Data are the means from 3 determinations ± S.E.M.

Mentions: As shown in Figure 7a–d, the effects of the polysaccharides on activation of human complement through the classical pathway (Figure 7a,b) and the alternative pathway (Figure 7c,d) were examined in 1:10-diluted NHS, with heparin used as a reference. The complement group (i.e., positive control) displayed a 93.11% ± 2.96% activation of the classical complement pathway. The activities of KCA, KCW, DKCA, DKCW and heparin were dose-dependent, while ds-DKCA and ds-DKCW showed little or no activity (Figure 7a,b). The activities of KCA and KCW reached a plateau at a concentration of 10 μg/mL, while DKCA plateaued at 50 μg/mL. In addition, the concentration that resulted in 50% inhibition of the classical complement pathway (CH50) for DKCW was approximately 218 μg/mL, which was lower than heparin. Therefore, KCA, KCW, and DKCA were more potent than heparin in inhibiting activation of the classical pathway. On the other hand, the concentrations of KCA, KCW, DKCA, DKCW and heparin that resulted in 50% inhibition of the alternative pathway (AP50) were 4.83, 18.60, 24.50, 19.97 and 137.25 μg/mL, respectively (Figure 7c,d). This finding indicated that KCA, KCW, DKCA and DKCW were more potent than heparin in inhibiting activation of the alternative pathway.


Structural analysis and anti-complement activity of polysaccharides from Kjellmaniella crsaaifolia.

Zhang W, Jin W, Sun D, Zhao L, Wang J, Duan D, Zhang Q - Mar Drugs (2015)

Inhibition of the classical pathway-mediated hemolysis of EA (a and b) and alternative pathway-mediated hemolysis of ER (c and d) in 1:10-diluted NHS in the presence of increasing amounts of the polysaccharides. Heparin was used as the reference. The results are expressed as percent inhibition of hemolysis. Data are the means from 3 determinations ± S.E.M.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4377988&req=5

marinedrugs-13-01360-f007: Inhibition of the classical pathway-mediated hemolysis of EA (a and b) and alternative pathway-mediated hemolysis of ER (c and d) in 1:10-diluted NHS in the presence of increasing amounts of the polysaccharides. Heparin was used as the reference. The results are expressed as percent inhibition of hemolysis. Data are the means from 3 determinations ± S.E.M.
Mentions: As shown in Figure 7a–d, the effects of the polysaccharides on activation of human complement through the classical pathway (Figure 7a,b) and the alternative pathway (Figure 7c,d) were examined in 1:10-diluted NHS, with heparin used as a reference. The complement group (i.e., positive control) displayed a 93.11% ± 2.96% activation of the classical complement pathway. The activities of KCA, KCW, DKCA, DKCW and heparin were dose-dependent, while ds-DKCA and ds-DKCW showed little or no activity (Figure 7a,b). The activities of KCA and KCW reached a plateau at a concentration of 10 μg/mL, while DKCA plateaued at 50 μg/mL. In addition, the concentration that resulted in 50% inhibition of the classical complement pathway (CH50) for DKCW was approximately 218 μg/mL, which was lower than heparin. Therefore, KCA, KCW, and DKCA were more potent than heparin in inhibiting activation of the classical pathway. On the other hand, the concentrations of KCA, KCW, DKCA, DKCW and heparin that resulted in 50% inhibition of the alternative pathway (AP50) were 4.83, 18.60, 24.50, 19.97 and 137.25 μg/mL, respectively (Figure 7c,d). This finding indicated that KCA, KCW, DKCA and DKCW were more potent than heparin in inhibiting activation of the alternative pathway.

Bottom Line: The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity.In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity.In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.

View Article: PubMed Central - PubMed

Affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wenjingwing@126.com.

ABSTRACT
Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.

No MeSH data available.


Related in: MedlinePlus