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Cytotoxic and antibacterial angucycline- and prodigiosin-analogues from the deep-sea derived Streptomyces sp. SCSIO 11594.

Song Y, Liu G, Li J, Huang H, Zhang X, Zhang H, Ju J - Mar Drugs (2015)

Bottom Line: SCSIO 11594.Compounds 1-3 were proved to have weak antibacterial activities against Enterococcus faecalis ATCC29212 with an MIC value of 64.0 μg/mL.Moreover, 3 displayed selective antibacterial activity against methicillin-resistant Staphylococcus epidermidis shhs-E1 with an MIC value of 16.0 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China. songx@scsio.ac.cn.

ABSTRACT
Two new C-glycoside angucyclines, marangucycline A (1) and marangucycline B (2), along with three known compounds, dehydroxyaquayamycin (3), undecylprodigiosin (4) and metacycloprodigiosin (5), have been identified as products of the deep-sea sediment strain Streptomyces sp. SCSIO 11594. New structures were elucidated on the basis of HRESIMS, 1D and 2D NMR analyses and comparisons to previously reported datasets. Compounds 2 and 4 displayed in vitro cytotoxicity against four cancer cell lines A594, CNE2, HepG2, MCF-7 superior to those obtained with cisplatin, the positive control. Notably, compound 2 bearing a keto-sugar displayed significant cytotoxicity against cancer cell lines with IC50 values ranging from 0.24 to 0.56 μM; An IC50 value of 3.67 μM was found when using non-cancerous hepatic cell line HL7702, demonstrating the cancer cell selectivity of 2. Compounds 1-3 were proved to have weak antibacterial activities against Enterococcus faecalis ATCC29212 with an MIC value of 64.0 μg/mL. Moreover, 3 displayed selective antibacterial activity against methicillin-resistant Staphylococcus epidermidis shhs-E1 with an MIC value of 16.0 μg/mL.

No MeSH data available.


Related in: MedlinePlus

(a) Strain SCSIO 11594; (b) Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain SCSIO 11594 and members of the family Streptomycetaceae. Bootstrap values (expressed as percentages of 1000 replications) exceeding 50% are shown at the branch points.
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marinedrugs-13-01304-f004: (a) Strain SCSIO 11594; (b) Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain SCSIO 11594 and members of the family Streptomycetaceae. Bootstrap values (expressed as percentages of 1000 replications) exceeding 50% are shown at the branch points.

Mentions: Strain SCSIO 11594 (Figure 4a was isolated from a sediment sample collected from the South China Sea (115°27.751 E, 19°28.581 N) at a depth of 2403 m using HRA medium (histidine 0.1 g, raffinose 1.0 g, Na2HPO4 0.3 g, KCl 1.7 g, MgSO4·7H2O 0.05 g, FeSO4·7H2O 0.01 g, CaCO3 0.02 g, agar 12 g, pH7.2, seawater 500 mL, distilled water 500 mL) with incubation at 28 °C for up to 4 weeks. The strain is preserved at the RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences. Extraction of genomic DNA, PCR amplification, sequencing of the 16S rRNA gene, and phylogenetic analysis were performed as described by You and co-workers [29]. The 16S rRNA gene sequence has been deposited in GenBank with accession no. KP276583. The results of phylogenetic analyses showed that strain SCSIO 11594 should be a member of the genus Streptomyces. The 16S rRNA gene sequence of strain SCSIO 11594 demonstrated the highest similarity value to Streptomyces rubrogriseus LMG 20318T (99.86%) and was found to cluster with members of the genus Streptomyces in the phylogenetic tree (Figure 4b).


Cytotoxic and antibacterial angucycline- and prodigiosin-analogues from the deep-sea derived Streptomyces sp. SCSIO 11594.

Song Y, Liu G, Li J, Huang H, Zhang X, Zhang H, Ju J - Mar Drugs (2015)

(a) Strain SCSIO 11594; (b) Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain SCSIO 11594 and members of the family Streptomycetaceae. Bootstrap values (expressed as percentages of 1000 replications) exceeding 50% are shown at the branch points.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4377985&req=5

marinedrugs-13-01304-f004: (a) Strain SCSIO 11594; (b) Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain SCSIO 11594 and members of the family Streptomycetaceae. Bootstrap values (expressed as percentages of 1000 replications) exceeding 50% are shown at the branch points.
Mentions: Strain SCSIO 11594 (Figure 4a was isolated from a sediment sample collected from the South China Sea (115°27.751 E, 19°28.581 N) at a depth of 2403 m using HRA medium (histidine 0.1 g, raffinose 1.0 g, Na2HPO4 0.3 g, KCl 1.7 g, MgSO4·7H2O 0.05 g, FeSO4·7H2O 0.01 g, CaCO3 0.02 g, agar 12 g, pH7.2, seawater 500 mL, distilled water 500 mL) with incubation at 28 °C for up to 4 weeks. The strain is preserved at the RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences. Extraction of genomic DNA, PCR amplification, sequencing of the 16S rRNA gene, and phylogenetic analysis were performed as described by You and co-workers [29]. The 16S rRNA gene sequence has been deposited in GenBank with accession no. KP276583. The results of phylogenetic analyses showed that strain SCSIO 11594 should be a member of the genus Streptomyces. The 16S rRNA gene sequence of strain SCSIO 11594 demonstrated the highest similarity value to Streptomyces rubrogriseus LMG 20318T (99.86%) and was found to cluster with members of the genus Streptomyces in the phylogenetic tree (Figure 4b).

Bottom Line: SCSIO 11594.Compounds 1-3 were proved to have weak antibacterial activities against Enterococcus faecalis ATCC29212 with an MIC value of 64.0 μg/mL.Moreover, 3 displayed selective antibacterial activity against methicillin-resistant Staphylococcus epidermidis shhs-E1 with an MIC value of 16.0 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China. songx@scsio.ac.cn.

ABSTRACT
Two new C-glycoside angucyclines, marangucycline A (1) and marangucycline B (2), along with three known compounds, dehydroxyaquayamycin (3), undecylprodigiosin (4) and metacycloprodigiosin (5), have been identified as products of the deep-sea sediment strain Streptomyces sp. SCSIO 11594. New structures were elucidated on the basis of HRESIMS, 1D and 2D NMR analyses and comparisons to previously reported datasets. Compounds 2 and 4 displayed in vitro cytotoxicity against four cancer cell lines A594, CNE2, HepG2, MCF-7 superior to those obtained with cisplatin, the positive control. Notably, compound 2 bearing a keto-sugar displayed significant cytotoxicity against cancer cell lines with IC50 values ranging from 0.24 to 0.56 μM; An IC50 value of 3.67 μM was found when using non-cancerous hepatic cell line HL7702, demonstrating the cancer cell selectivity of 2. Compounds 1-3 were proved to have weak antibacterial activities against Enterococcus faecalis ATCC29212 with an MIC value of 64.0 μg/mL. Moreover, 3 displayed selective antibacterial activity against methicillin-resistant Staphylococcus epidermidis shhs-E1 with an MIC value of 16.0 μg/mL.

No MeSH data available.


Related in: MedlinePlus