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Effects of nuclear genomes on anther development in cytoplasmic male sterile chicories (Cichorium intybus L.): morphological analysis.

Habarugira I, Hendriks T, Quillet MC, Hilbert JL, Rambaud C - ScientificWorldJournal (2015)

Bottom Line: In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted.Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions.Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer.

View Article: PubMed Central - PubMed

Affiliation: UMR 1281, Stress Abiotiques et Différenciation des Végétaux Cultivés, Université Lille 1, Sciences et Technologies, 59655 Villeneuve-d'Ascq, France ; University of Rwanda-Collège of Education, P.O. Box 5039, Kigali, Rwanda.

ABSTRACT
The Cichorium intybus flower development in fertile, cytoplasmic male sterility (CMS 524) and various phenotypes carrying the 524 male sterile cytoplasm was investigated macroscopically and by light microscopy. The development was similar in fertile and in male sterile florets up to meiosis, and then it was affected in anther wall structure and pollen grain development in male sterile floret. In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted. Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions. Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer.

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Related in: MedlinePlus

Overview of inflorescence and fertile flower structure. (a) Various stages of capitulum development on a branch with an opened capitulum at anthesis showing ligulate florets. Inset: capitulum with the external bracts partially removed prior to length measurement. (b) The anthers form a column through which the stylar branches and style move upward brushing the pollen grains. The stamens are attached to the ligule and the inferior ovary bears a scaly pappus. Bars (a) 1 cm, inset 1 mm, (b) 1 mm.
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Related In: Results  -  Collection


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fig2: Overview of inflorescence and fertile flower structure. (a) Various stages of capitulum development on a branch with an opened capitulum at anthesis showing ligulate florets. Inset: capitulum with the external bracts partially removed prior to length measurement. (b) The anthers form a column through which the stylar branches and style move upward brushing the pollen grains. The stamens are attached to the ligule and the inferior ovary bears a scaly pappus. Bars (a) 1 cm, inset 1 mm, (b) 1 mm.

Mentions: As in other Asteraceae, the flowers of C. intybus are aggregated into heads. At anthesis the capitulum comprises 15–25 hermaphrodite ligulate florets (Figure 2(a)) surrounded by involucral bracts. The anthers are blue and white and form a column surrounding the style (Figure 2(b)). Each of the five stamens is attached to the corolla by the base of its filament. The pistil comprises a unilocular ovary and a style terminating in two (sometimes three) stylar branches (Figure 2(b)). The calyx is reduced to pappus consisting of small scales and the corolla consists of a ligule with five fused petals (Figure 2(b)). The flower development lasts approximately 15 days from flower initiation to anthesis and every capitulum opens only for one day, all the florets being synchronously opened (communication from Theo Hendriks and David Gagneul).


Effects of nuclear genomes on anther development in cytoplasmic male sterile chicories (Cichorium intybus L.): morphological analysis.

Habarugira I, Hendriks T, Quillet MC, Hilbert JL, Rambaud C - ScientificWorldJournal (2015)

Overview of inflorescence and fertile flower structure. (a) Various stages of capitulum development on a branch with an opened capitulum at anthesis showing ligulate florets. Inset: capitulum with the external bracts partially removed prior to length measurement. (b) The anthers form a column through which the stylar branches and style move upward brushing the pollen grains. The stamens are attached to the ligule and the inferior ovary bears a scaly pappus. Bars (a) 1 cm, inset 1 mm, (b) 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4377467&req=5

fig2: Overview of inflorescence and fertile flower structure. (a) Various stages of capitulum development on a branch with an opened capitulum at anthesis showing ligulate florets. Inset: capitulum with the external bracts partially removed prior to length measurement. (b) The anthers form a column through which the stylar branches and style move upward brushing the pollen grains. The stamens are attached to the ligule and the inferior ovary bears a scaly pappus. Bars (a) 1 cm, inset 1 mm, (b) 1 mm.
Mentions: As in other Asteraceae, the flowers of C. intybus are aggregated into heads. At anthesis the capitulum comprises 15–25 hermaphrodite ligulate florets (Figure 2(a)) surrounded by involucral bracts. The anthers are blue and white and form a column surrounding the style (Figure 2(b)). Each of the five stamens is attached to the corolla by the base of its filament. The pistil comprises a unilocular ovary and a style terminating in two (sometimes three) stylar branches (Figure 2(b)). The calyx is reduced to pappus consisting of small scales and the corolla consists of a ligule with five fused petals (Figure 2(b)). The flower development lasts approximately 15 days from flower initiation to anthesis and every capitulum opens only for one day, all the florets being synchronously opened (communication from Theo Hendriks and David Gagneul).

Bottom Line: In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted.Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions.Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer.

View Article: PubMed Central - PubMed

Affiliation: UMR 1281, Stress Abiotiques et Différenciation des Végétaux Cultivés, Université Lille 1, Sciences et Technologies, 59655 Villeneuve-d'Ascq, France ; University of Rwanda-Collège of Education, P.O. Box 5039, Kigali, Rwanda.

ABSTRACT
The Cichorium intybus flower development in fertile, cytoplasmic male sterility (CMS 524) and various phenotypes carrying the 524 male sterile cytoplasm was investigated macroscopically and by light microscopy. The development was similar in fertile and in male sterile florets up to meiosis, and then it was affected in anther wall structure and pollen grain development in male sterile floret. In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted. Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions. Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer.

Show MeSH
Related in: MedlinePlus