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Inflammatory Cytokine Expression and Sebum Production after Exposure of Cultured Human Sebocytes to Ultraviolet A Radiation and Light at Wavelengths of 650 nm and 830 nm.

Lee WJ, Chae SY, Ryu HS, Jang YH, Lee SJ, Kim do W - Ann Dermatol (2015)

Bottom Line: The effectiveness of ultraviolet (UV) radiation, visible light, or infrared light therapy for the treatment of acne is the subject of ongoing scientific debate.Expression of inflammatory cytokine proteins and sebum production were measured using enzyme-linked immunoassays and a lipid analysis kit, respectively.Exposure of cultured sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm did not show a significant increase in the expression of inflammatory cytokines, MMPs, or AMPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.

ABSTRACT

Background: The effectiveness of ultraviolet (UV) radiation, visible light, or infrared light therapy for the treatment of acne is the subject of ongoing scientific debate.

Objective: This study was conducted to investigate changes in sebum production and the expression of inflammatory cytokines, matrix metalloproteinases (MMPs), and antimicrobial peptides (AMPs), following exposure of cultured human sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm.

Methods: Reverse transcription polymerase chain reaction assays were performed to measure the gene expression levels of inflammatory cytokines (interleukin [IL]-1β, IL-6, IL-8, and tumor necrosis factor-α), MMPs (MMP-1, MMP-3, and MMP-9), and AMPs (psoriasin, hBD-2, hBD-3, and LL-37) in cultured sebocytes after exposure to UVA radiation (2 J/cm(2), 3 J/cm(2), and 5 J/cm(2)) and light at wavelengths of 650 nm (14 J/cm(2), 29 J/cm(2), and 87 J/cm(2)) and 830 nm (5 J/cm(2), 10 J/cm(2), and 30 J/cm(2)). Expression of inflammatory cytokine proteins and sebum production were measured using enzyme-linked immunoassays and a lipid analysis kit, respectively.

Results: Exposure of cultured sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm did not show a significant increase in the expression of inflammatory cytokines, MMPs, or AMPs. Sebum production was not significantly decreased after exposure to UVA radiation and light at both wavelengths.

Conclusion: We propose that UVA radiation, visible light, and infrared light can be used to target Propionibacterium acnes for the treatment of acne, without an increase in the expression of inflammatory biomarkers and sebum production.

No MeSH data available.


Related in: MedlinePlus

The protein expression levels of interleukin (IL)-1β, IL-6, and IL-8 in cultured human sebocytes treated with ultraviolet A (UVA) radiation and light at wavelengths of 650 nm and 830 nm did not show significant change compared to those in the controls. The expression of tumor necrosis factor (TNF)-α protein was insignificantly decreased in sebocytes treated with UVA radiation and light at a wavelength of 650 nm compared to the control. Expression levels are presented as relative ratios. Error bars represent standard errors calculated based on three independent experiments.
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Figure 2: The protein expression levels of interleukin (IL)-1β, IL-6, and IL-8 in cultured human sebocytes treated with ultraviolet A (UVA) radiation and light at wavelengths of 650 nm and 830 nm did not show significant change compared to those in the controls. The expression of tumor necrosis factor (TNF)-α protein was insignificantly decreased in sebocytes treated with UVA radiation and light at a wavelength of 650 nm compared to the control. Expression levels are presented as relative ratios. Error bars represent standard errors calculated based on three independent experiments.

Mentions: The gene and protein expression of IL-1β, IL-6, and IL-8 in cultured sebocytes treated with UVA radiation (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant increase compared to that observed in the control (Fig. 1, 2). However, TNF-α gene expression levels in sebocytes treated with 3 J/cm2 and 5 J/cm2 of UVA showed a significant decrease compared to that in the control (p<0.05) (Fig. 1). This was not reflected in the levels of TNF-α protein expression, which did not show a significant decrease compared to that in the control (Fig. 2). The gene expression levels of MMP-1 and MMP-3 in UVA-treated cells (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant increase compared to those in the control (Fig. 1). In contrast, MMP-9 gene expression showed a significant decrease in UVA-treated cells (2 J/cm2, 3 J/cm2, and 5 J/cm2) compared to that in the control (p<0.05) (Fig. 1). The expression levels of AMPs, including psoriasin, hBD-2, hBD-3, and LL-37, in UVA-treated sebocytes (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant change compared to those in the control (Fig. 1).


Inflammatory Cytokine Expression and Sebum Production after Exposure of Cultured Human Sebocytes to Ultraviolet A Radiation and Light at Wavelengths of 650 nm and 830 nm.

Lee WJ, Chae SY, Ryu HS, Jang YH, Lee SJ, Kim do W - Ann Dermatol (2015)

The protein expression levels of interleukin (IL)-1β, IL-6, and IL-8 in cultured human sebocytes treated with ultraviolet A (UVA) radiation and light at wavelengths of 650 nm and 830 nm did not show significant change compared to those in the controls. The expression of tumor necrosis factor (TNF)-α protein was insignificantly decreased in sebocytes treated with UVA radiation and light at a wavelength of 650 nm compared to the control. Expression levels are presented as relative ratios. Error bars represent standard errors calculated based on three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4377405&req=5

Figure 2: The protein expression levels of interleukin (IL)-1β, IL-6, and IL-8 in cultured human sebocytes treated with ultraviolet A (UVA) radiation and light at wavelengths of 650 nm and 830 nm did not show significant change compared to those in the controls. The expression of tumor necrosis factor (TNF)-α protein was insignificantly decreased in sebocytes treated with UVA radiation and light at a wavelength of 650 nm compared to the control. Expression levels are presented as relative ratios. Error bars represent standard errors calculated based on three independent experiments.
Mentions: The gene and protein expression of IL-1β, IL-6, and IL-8 in cultured sebocytes treated with UVA radiation (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant increase compared to that observed in the control (Fig. 1, 2). However, TNF-α gene expression levels in sebocytes treated with 3 J/cm2 and 5 J/cm2 of UVA showed a significant decrease compared to that in the control (p<0.05) (Fig. 1). This was not reflected in the levels of TNF-α protein expression, which did not show a significant decrease compared to that in the control (Fig. 2). The gene expression levels of MMP-1 and MMP-3 in UVA-treated cells (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant increase compared to those in the control (Fig. 1). In contrast, MMP-9 gene expression showed a significant decrease in UVA-treated cells (2 J/cm2, 3 J/cm2, and 5 J/cm2) compared to that in the control (p<0.05) (Fig. 1). The expression levels of AMPs, including psoriasin, hBD-2, hBD-3, and LL-37, in UVA-treated sebocytes (2 J/cm2, 3 J/cm2, and 5 J/cm2) did not show a significant change compared to those in the control (Fig. 1).

Bottom Line: The effectiveness of ultraviolet (UV) radiation, visible light, or infrared light therapy for the treatment of acne is the subject of ongoing scientific debate.Expression of inflammatory cytokine proteins and sebum production were measured using enzyme-linked immunoassays and a lipid analysis kit, respectively.Exposure of cultured sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm did not show a significant increase in the expression of inflammatory cytokines, MMPs, or AMPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.

ABSTRACT

Background: The effectiveness of ultraviolet (UV) radiation, visible light, or infrared light therapy for the treatment of acne is the subject of ongoing scientific debate.

Objective: This study was conducted to investigate changes in sebum production and the expression of inflammatory cytokines, matrix metalloproteinases (MMPs), and antimicrobial peptides (AMPs), following exposure of cultured human sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm.

Methods: Reverse transcription polymerase chain reaction assays were performed to measure the gene expression levels of inflammatory cytokines (interleukin [IL]-1β, IL-6, IL-8, and tumor necrosis factor-α), MMPs (MMP-1, MMP-3, and MMP-9), and AMPs (psoriasin, hBD-2, hBD-3, and LL-37) in cultured sebocytes after exposure to UVA radiation (2 J/cm(2), 3 J/cm(2), and 5 J/cm(2)) and light at wavelengths of 650 nm (14 J/cm(2), 29 J/cm(2), and 87 J/cm(2)) and 830 nm (5 J/cm(2), 10 J/cm(2), and 30 J/cm(2)). Expression of inflammatory cytokine proteins and sebum production were measured using enzyme-linked immunoassays and a lipid analysis kit, respectively.

Results: Exposure of cultured sebocytes to UVA radiation and light at wavelengths of 650 nm and 830 nm did not show a significant increase in the expression of inflammatory cytokines, MMPs, or AMPs. Sebum production was not significantly decreased after exposure to UVA radiation and light at both wavelengths.

Conclusion: We propose that UVA radiation, visible light, and infrared light can be used to target Propionibacterium acnes for the treatment of acne, without an increase in the expression of inflammatory biomarkers and sebum production.

No MeSH data available.


Related in: MedlinePlus