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In vitro antimicrobial and modulatory activity of the natural products silymarin and silibinin.

de Oliveira DR, Tintino SR, Braga MF, Boligon AA, Athayde ML, Coutinho HD, de Menezes IR, Fachinetto R - Biomed Res Int (2015)

Bottom Line: The aim of this study was to investigate the antibacterial and antifungal activity of silymarin and its major constituent (silibinin) against different microbial strains and their modulatory effect on drugs utilized in clinical practice.The results for the antifungal activity of silymarin and silibinin demonstrated a MIC of 1024 µg/mL for all strains.Silymarin and silibinin appear to have promising potential, showing synergistic properties when combined with antibacterial drugs, which should prompt further studies along this line.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-700 Santa Maria, RS, Brazil.

ABSTRACT
Silymarin is a standardized extract from the dried seeds of the milk thistle (Silybum marianum L. Gaertn.) clinically used as an antihepatotoxic agent. The aim of this study was to investigate the antibacterial and antifungal activity of silymarin and its major constituent (silibinin) against different microbial strains and their modulatory effect on drugs utilized in clinical practice. Silymarin demonstrated antimicrobial activity of little significance against the bacterial strains tested, with MIC (minimum inhibitory concentration) values of 512 µg/mL. Meanwhile, silibinin showed significant activity against Escherichia coli with a MIC of 64 µg/mL. The results for the antifungal activity of silymarin and silibinin demonstrated a MIC of 1024 µg/mL for all strains. Silymarin and silibinin appear to have promising potential, showing synergistic properties when combined with antibacterial drugs, which should prompt further studies along this line.

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Related in: MedlinePlus

MIC (μg/mL) of the antibiotics in the absence and presence of silymarin and silibinin at subinhibitory concentrations for E. coli strain EC06.
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Related In: Results  -  Collection


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fig2: MIC (μg/mL) of the antibiotics in the absence and presence of silymarin and silibinin at subinhibitory concentrations for E. coli strain EC06.

Mentions: Silymarin demonstrated antimicrobial activity that was clinically irrelevant, with MIC values of 512 μg/mL. The results demonstrating the modulatory antibiotic activity of silymarin and silibinin are demonstrated in Figures 2–4 and the silymarin at a concentration of 64 μg/mL was combined with the antibiotics. Silibinin showed a MIC of 1024 μg/mL and was thus clinically irrelevant for the strains Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 9027, so a concentration of 128 μg/mL was used in drug-modifying assays. However, for Escherichia coli ATCC 25922, the MIC was 64 μg/mL, and thus, a concentration of 8 μg/mL was used in drug-modifying assays.


In vitro antimicrobial and modulatory activity of the natural products silymarin and silibinin.

de Oliveira DR, Tintino SR, Braga MF, Boligon AA, Athayde ML, Coutinho HD, de Menezes IR, Fachinetto R - Biomed Res Int (2015)

MIC (μg/mL) of the antibiotics in the absence and presence of silymarin and silibinin at subinhibitory concentrations for E. coli strain EC06.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4377387&req=5

fig2: MIC (μg/mL) of the antibiotics in the absence and presence of silymarin and silibinin at subinhibitory concentrations for E. coli strain EC06.
Mentions: Silymarin demonstrated antimicrobial activity that was clinically irrelevant, with MIC values of 512 μg/mL. The results demonstrating the modulatory antibiotic activity of silymarin and silibinin are demonstrated in Figures 2–4 and the silymarin at a concentration of 64 μg/mL was combined with the antibiotics. Silibinin showed a MIC of 1024 μg/mL and was thus clinically irrelevant for the strains Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 9027, so a concentration of 128 μg/mL was used in drug-modifying assays. However, for Escherichia coli ATCC 25922, the MIC was 64 μg/mL, and thus, a concentration of 8 μg/mL was used in drug-modifying assays.

Bottom Line: The aim of this study was to investigate the antibacterial and antifungal activity of silymarin and its major constituent (silibinin) against different microbial strains and their modulatory effect on drugs utilized in clinical practice.The results for the antifungal activity of silymarin and silibinin demonstrated a MIC of 1024 µg/mL for all strains.Silymarin and silibinin appear to have promising potential, showing synergistic properties when combined with antibacterial drugs, which should prompt further studies along this line.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-700 Santa Maria, RS, Brazil.

ABSTRACT
Silymarin is a standardized extract from the dried seeds of the milk thistle (Silybum marianum L. Gaertn.) clinically used as an antihepatotoxic agent. The aim of this study was to investigate the antibacterial and antifungal activity of silymarin and its major constituent (silibinin) against different microbial strains and their modulatory effect on drugs utilized in clinical practice. Silymarin demonstrated antimicrobial activity of little significance against the bacterial strains tested, with MIC (minimum inhibitory concentration) values of 512 µg/mL. Meanwhile, silibinin showed significant activity against Escherichia coli with a MIC of 64 µg/mL. The results for the antifungal activity of silymarin and silibinin demonstrated a MIC of 1024 µg/mL for all strains. Silymarin and silibinin appear to have promising potential, showing synergistic properties when combined with antibacterial drugs, which should prompt further studies along this line.

Show MeSH
Related in: MedlinePlus