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Identification and expression analysis of the interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) gene in duck (Anas platyrhynchos domesticus).

Wang B, Chen Y, Mu C, Su Y, Liu R, Huang Z, Li Y, Yu Q, Chang G, Xu Q, Chen G - PLoS ONE (2015)

Bottom Line: Based on the sequence obtained, we performed a series of bioinformatics analyses, and found that duIFIT5 was most similar to homologs in other avian species.Finally, we used duck hepatitis virus type 1 (DHV-1) and polyriboinosinicpolyribocytidylic acid (poly (I:C)) as a pathogen or a pathogen-associated molecular pattern induction to infect three-day-old domestic ducklings.Taken together, these results provide a greater understanding of avian IFIT5.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.

ABSTRACT
The interferon-induced proteins with tetratricopeptide repeats (IFITs) protein family mediates antiviral effects by inhibiting translation initiation, cell proliferation, and migration in the interferon (IFN) dependent innate immune system. Several members of this family, including IFIT1, IFIT2, IFIT3 and IFIT5, have been heavily studied in mammals. Avian species contain only one family member, IFIT5, and little is known about the role of this protein in birds. In this study, duck IFIT5 (duIFIT5) full-length mRNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). Based on the sequence obtained, we performed a series of bioinformatics analyses, and found that duIFIT5 was most similar to homologs in other avian species. Also, duIFIT5 contained eight conserved TPR motifs and two conserved multi-domains (TPR_11 and TPR_12). Finally, we used duck hepatitis virus type 1 (DHV-1) and polyriboinosinicpolyribocytidylic acid (poly (I:C)) as a pathogen or a pathogen-associated molecular pattern induction to infect three-day-old domestic ducklings. The liver and spleen were collected to detect the change in duIFIT5 transcript level upon infection by quantitative real-time PCR (qRT-PCR). DuIFIT5 expression rapidly increased after DHV-1 infection and maintained a high level, while the transcripts of duIFIT5 peaked at 8h after poly (I:C) infection and then returned to normal. Taken together, these results provide a greater understanding of avian IFIT5.

No MeSH data available.


Related in: MedlinePlus

Subcellular localization of duIFIT5 in DF1 cells.EGFP-C1-duIFIT5 or an EGFP-C1 control plasmid was transiently transfected into DF1 cells, shown in A and B, respectively. DuIFIT5 was mostly in the cytoplasmic, but was also detected in the nucleus.
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pone.0121065.g003: Subcellular localization of duIFIT5 in DF1 cells.EGFP-C1-duIFIT5 or an EGFP-C1 control plasmid was transiently transfected into DF1 cells, shown in A and B, respectively. DuIFIT5 was mostly in the cytoplasmic, but was also detected in the nucleus.

Mentions: To confirm the above prediction, the EGFP-C1-duIFIT5 plasmid, or EGFP-C1 as a negative control, was transfected into chicken embryo fibroblasts (DF1 cells), and fluorescence was observed 24 h post-transfection (Fig. 3). DuIFIT5 was predominantly in the cytoplasmic, but was also visualized in the nucleus.


Identification and expression analysis of the interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) gene in duck (Anas platyrhynchos domesticus).

Wang B, Chen Y, Mu C, Su Y, Liu R, Huang Z, Li Y, Yu Q, Chang G, Xu Q, Chen G - PLoS ONE (2015)

Subcellular localization of duIFIT5 in DF1 cells.EGFP-C1-duIFIT5 or an EGFP-C1 control plasmid was transiently transfected into DF1 cells, shown in A and B, respectively. DuIFIT5 was mostly in the cytoplasmic, but was also detected in the nucleus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376821&req=5

pone.0121065.g003: Subcellular localization of duIFIT5 in DF1 cells.EGFP-C1-duIFIT5 or an EGFP-C1 control plasmid was transiently transfected into DF1 cells, shown in A and B, respectively. DuIFIT5 was mostly in the cytoplasmic, but was also detected in the nucleus.
Mentions: To confirm the above prediction, the EGFP-C1-duIFIT5 plasmid, or EGFP-C1 as a negative control, was transfected into chicken embryo fibroblasts (DF1 cells), and fluorescence was observed 24 h post-transfection (Fig. 3). DuIFIT5 was predominantly in the cytoplasmic, but was also visualized in the nucleus.

Bottom Line: Based on the sequence obtained, we performed a series of bioinformatics analyses, and found that duIFIT5 was most similar to homologs in other avian species.Finally, we used duck hepatitis virus type 1 (DHV-1) and polyriboinosinicpolyribocytidylic acid (poly (I:C)) as a pathogen or a pathogen-associated molecular pattern induction to infect three-day-old domestic ducklings.Taken together, these results provide a greater understanding of avian IFIT5.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.

ABSTRACT
The interferon-induced proteins with tetratricopeptide repeats (IFITs) protein family mediates antiviral effects by inhibiting translation initiation, cell proliferation, and migration in the interferon (IFN) dependent innate immune system. Several members of this family, including IFIT1, IFIT2, IFIT3 and IFIT5, have been heavily studied in mammals. Avian species contain only one family member, IFIT5, and little is known about the role of this protein in birds. In this study, duck IFIT5 (duIFIT5) full-length mRNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). Based on the sequence obtained, we performed a series of bioinformatics analyses, and found that duIFIT5 was most similar to homologs in other avian species. Also, duIFIT5 contained eight conserved TPR motifs and two conserved multi-domains (TPR_11 and TPR_12). Finally, we used duck hepatitis virus type 1 (DHV-1) and polyriboinosinicpolyribocytidylic acid (poly (I:C)) as a pathogen or a pathogen-associated molecular pattern induction to infect three-day-old domestic ducklings. The liver and spleen were collected to detect the change in duIFIT5 transcript level upon infection by quantitative real-time PCR (qRT-PCR). DuIFIT5 expression rapidly increased after DHV-1 infection and maintained a high level, while the transcripts of duIFIT5 peaked at 8h after poly (I:C) infection and then returned to normal. Taken together, these results provide a greater understanding of avian IFIT5.

No MeSH data available.


Related in: MedlinePlus