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Changes in ovaries and uterus after aglepristone administration in the third week of luteal phase of non-pregnant bitches.

Kacprzak K, Jurka P, Dolka I, Czopowicz M, Ruszczak A, Duszewska A - PLoS ONE (2015)

Bottom Line: A decrease of progesterone (p<0.01) and no differences in total estrogen level in the study group were observed.There were no significant differences either in the histomorphometry or α-estrogen and progesterone receptors expression in ovaries.No differences were observed between endometrium of horns and body within groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Small Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.

ABSTRACT

Objective: The mechanism of aglepristone action in the placentation time in the bitch remains unclear. The aim of this study was to describe the mechanism by which aglepristone influences ovaries and uterus and to measure the levels of steroid sex hormones in non-pregnant bitches.

Materials and methods: Fourteen bitches assigned to a study (n=9) and control (n=5) group were given aglepristone and saline solution, respectively, on the 19th and 20th day after LH peak. On the 26th day after LH peak an ovariohysterectomy was performed. Blood samples were screened for estradiol and progesterone concentrations. Ovaries and uterine horns and bodies were isolated for histological and morphometrical diagnosis and immunohistochemistry analysis of α-estrogen and progesterone receptor expression.

Results: A decrease of progesterone (p<0.01) and no differences in total estrogen level in the study group were observed. There were no significant differences either in the histomorphometry or α-estrogen and progesterone receptors expression in ovaries. Increase in expression of progesterone receptors in endometrium without surface epithelium of horns (p<0.05), endometrial surface epithelium (p<0.05), myometrium of uterine body (p<0.01) and estrogen receptors in endometrium without surface epithelium of horns (p<0.05) was observed. Elevated estrogen receptors probably increased sensitivity of tissues to estrogens in the bloodstream and led to notable inflammation, haemorrhages, and hyperplasia in endometrium with mononuclear immune cell infiltration. The myometrium of horns and endometrium of uterine body of study bitches were significantly thicker than in the control group (p<0.05 and p<0.01). Furthermore myometrium of uterine body was thicker than myometrium of horns (p<0.001) and expression of progesterone receptors was higher in uterine body (p<0.01). No differences were observed between endometrium of horns and body within groups.

Conclusion: To the knowledge of the authors this is the first study, which describes the inflammatory effect developing in uterus in response to aglepristone administration, and attempts to elucidate its mechanisms.

No MeSH data available.


Related in: MedlinePlus

Serum estradiol concentrations in study (n = 9) and control (n = 5) bitches during the luteal phase, from days 8 to 27 after the estimated LH peak.The AA indicates days of treatment with aglepristone (10 mg/kg bw) or saline and O it is the day of castration. Results are expressed as means ± SD.
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pone.0121597.g002: Serum estradiol concentrations in study (n = 9) and control (n = 5) bitches during the luteal phase, from days 8 to 27 after the estimated LH peak.The AA indicates days of treatment with aglepristone (10 mg/kg bw) or saline and O it is the day of castration. Results are expressed as means ± SD.

Mentions: The mean serum E concentrations were relatively high before RU534 administration (124.4 ± 25.0 pmol/l), but gradually declined to 88.1–99.1 pmol/l on day 27 after LH peak, remaining at approximately the same levels thereafter (Fig. 2). Serum E levels did not differ in study and control bitches before and after RU534 administration for the entire study (Fig. 2).


Changes in ovaries and uterus after aglepristone administration in the third week of luteal phase of non-pregnant bitches.

Kacprzak K, Jurka P, Dolka I, Czopowicz M, Ruszczak A, Duszewska A - PLoS ONE (2015)

Serum estradiol concentrations in study (n = 9) and control (n = 5) bitches during the luteal phase, from days 8 to 27 after the estimated LH peak.The AA indicates days of treatment with aglepristone (10 mg/kg bw) or saline and O it is the day of castration. Results are expressed as means ± SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376714&req=5

pone.0121597.g002: Serum estradiol concentrations in study (n = 9) and control (n = 5) bitches during the luteal phase, from days 8 to 27 after the estimated LH peak.The AA indicates days of treatment with aglepristone (10 mg/kg bw) or saline and O it is the day of castration. Results are expressed as means ± SD.
Mentions: The mean serum E concentrations were relatively high before RU534 administration (124.4 ± 25.0 pmol/l), but gradually declined to 88.1–99.1 pmol/l on day 27 after LH peak, remaining at approximately the same levels thereafter (Fig. 2). Serum E levels did not differ in study and control bitches before and after RU534 administration for the entire study (Fig. 2).

Bottom Line: A decrease of progesterone (p<0.01) and no differences in total estrogen level in the study group were observed.There were no significant differences either in the histomorphometry or α-estrogen and progesterone receptors expression in ovaries.No differences were observed between endometrium of horns and body within groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Small Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.

ABSTRACT

Objective: The mechanism of aglepristone action in the placentation time in the bitch remains unclear. The aim of this study was to describe the mechanism by which aglepristone influences ovaries and uterus and to measure the levels of steroid sex hormones in non-pregnant bitches.

Materials and methods: Fourteen bitches assigned to a study (n=9) and control (n=5) group were given aglepristone and saline solution, respectively, on the 19th and 20th day after LH peak. On the 26th day after LH peak an ovariohysterectomy was performed. Blood samples were screened for estradiol and progesterone concentrations. Ovaries and uterine horns and bodies were isolated for histological and morphometrical diagnosis and immunohistochemistry analysis of α-estrogen and progesterone receptor expression.

Results: A decrease of progesterone (p<0.01) and no differences in total estrogen level in the study group were observed. There were no significant differences either in the histomorphometry or α-estrogen and progesterone receptors expression in ovaries. Increase in expression of progesterone receptors in endometrium without surface epithelium of horns (p<0.05), endometrial surface epithelium (p<0.05), myometrium of uterine body (p<0.01) and estrogen receptors in endometrium without surface epithelium of horns (p<0.05) was observed. Elevated estrogen receptors probably increased sensitivity of tissues to estrogens in the bloodstream and led to notable inflammation, haemorrhages, and hyperplasia in endometrium with mononuclear immune cell infiltration. The myometrium of horns and endometrium of uterine body of study bitches were significantly thicker than in the control group (p<0.05 and p<0.01). Furthermore myometrium of uterine body was thicker than myometrium of horns (p<0.001) and expression of progesterone receptors was higher in uterine body (p<0.01). No differences were observed between endometrium of horns and body within groups.

Conclusion: To the knowledge of the authors this is the first study, which describes the inflammatory effect developing in uterus in response to aglepristone administration, and attempts to elucidate its mechanisms.

No MeSH data available.


Related in: MedlinePlus