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Analysis of the cytotoxicity of carbon-based nanoparticles, diamond and graphite, in human glioblastoma and hepatoma cell lines.

Zakrzewska KE, Samluk A, Wierzbicki M, Jaworski S, Kutwin M, Sawosz E, Chwalibog A, Pijanowska DG, Pluta KD - PLoS ONE (2015)

Bottom Line: First, we confirmed previous results that diamond nanoparticles are practically nontoxic.The studied carbon nanoparticles could be a potentially useful tool for therapeutics delivery to the brain tissue with minimal side effects on the hepatocytes.Furthermore, we showed the influence of the nanoparticles on the stable, fluorescently labeled tumor cell lines and concluded that the labeled cells are suitable for drug cytotoxicity tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Hybrid Microbiosystem Engineering, Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland.

ABSTRACT
Nanoparticles have attracted a great deal of attention as carriers for drug delivery to cancer cells. However, reports on their potential cytotoxicity raise questions of their safety and this matter needs attentive consideration. In this paper, for the first time, the cytotoxic effects of two carbon based nanoparticles, diamond and graphite, on glioblastoma and hepatoma cells were compared. First, we confirmed previous results that diamond nanoparticles are practically nontoxic. Second, graphite nanoparticles exhibited a negative impact on glioblastoma, but not on hepatoma cells. The studied carbon nanoparticles could be a potentially useful tool for therapeutics delivery to the brain tissue with minimal side effects on the hepatocytes. Furthermore, we showed the influence of the nanoparticles on the stable, fluorescently labeled tumor cell lines and concluded that the labeled cells are suitable for drug cytotoxicity tests.

No MeSH data available.


Related in: MedlinePlus

Glioblastoma and hepatoma cells morphology.U87, U87-EGFP, C3A and C3A-EGFP cells morphology after 2 h exposure to medium containing nanoparticles at the highest concentration: graphite 100 μg/mL (NG 100) and diamond 100 μg/mL (ND 100), nontreated cells were used as a control (Control). Analysis of the cells morphology was performed using an inverted fluorescence microscope. Images of the labeled cells, U87-EGFP and C3A-EGFP, were captured using a green fluorescence filter. Scale bar: 200 μm.
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pone.0122579.g002: Glioblastoma and hepatoma cells morphology.U87, U87-EGFP, C3A and C3A-EGFP cells morphology after 2 h exposure to medium containing nanoparticles at the highest concentration: graphite 100 μg/mL (NG 100) and diamond 100 μg/mL (ND 100), nontreated cells were used as a control (Control). Analysis of the cells morphology was performed using an inverted fluorescence microscope. Images of the labeled cells, U87-EGFP and C3A-EGFP, were captured using a green fluorescence filter. Scale bar: 200 μm.

Mentions: The morphology of the tumor cell lines was analyzed using an inverted fluorescence microscope 2 and 24 h after exposure to ND and NG. Regardless of the incubation time, we did not observe any differences in cell morphology compared to the nontreated cells. The NPs appeared on the bright field microphotographs as black dots, aggregated inside the cells or on the cells surface (Fig 2).


Analysis of the cytotoxicity of carbon-based nanoparticles, diamond and graphite, in human glioblastoma and hepatoma cell lines.

Zakrzewska KE, Samluk A, Wierzbicki M, Jaworski S, Kutwin M, Sawosz E, Chwalibog A, Pijanowska DG, Pluta KD - PLoS ONE (2015)

Glioblastoma and hepatoma cells morphology.U87, U87-EGFP, C3A and C3A-EGFP cells morphology after 2 h exposure to medium containing nanoparticles at the highest concentration: graphite 100 μg/mL (NG 100) and diamond 100 μg/mL (ND 100), nontreated cells were used as a control (Control). Analysis of the cells morphology was performed using an inverted fluorescence microscope. Images of the labeled cells, U87-EGFP and C3A-EGFP, were captured using a green fluorescence filter. Scale bar: 200 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376528&req=5

pone.0122579.g002: Glioblastoma and hepatoma cells morphology.U87, U87-EGFP, C3A and C3A-EGFP cells morphology after 2 h exposure to medium containing nanoparticles at the highest concentration: graphite 100 μg/mL (NG 100) and diamond 100 μg/mL (ND 100), nontreated cells were used as a control (Control). Analysis of the cells morphology was performed using an inverted fluorescence microscope. Images of the labeled cells, U87-EGFP and C3A-EGFP, were captured using a green fluorescence filter. Scale bar: 200 μm.
Mentions: The morphology of the tumor cell lines was analyzed using an inverted fluorescence microscope 2 and 24 h after exposure to ND and NG. Regardless of the incubation time, we did not observe any differences in cell morphology compared to the nontreated cells. The NPs appeared on the bright field microphotographs as black dots, aggregated inside the cells or on the cells surface (Fig 2).

Bottom Line: First, we confirmed previous results that diamond nanoparticles are practically nontoxic.The studied carbon nanoparticles could be a potentially useful tool for therapeutics delivery to the brain tissue with minimal side effects on the hepatocytes.Furthermore, we showed the influence of the nanoparticles on the stable, fluorescently labeled tumor cell lines and concluded that the labeled cells are suitable for drug cytotoxicity tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Hybrid Microbiosystem Engineering, Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland.

ABSTRACT
Nanoparticles have attracted a great deal of attention as carriers for drug delivery to cancer cells. However, reports on their potential cytotoxicity raise questions of their safety and this matter needs attentive consideration. In this paper, for the first time, the cytotoxic effects of two carbon based nanoparticles, diamond and graphite, on glioblastoma and hepatoma cells were compared. First, we confirmed previous results that diamond nanoparticles are practically nontoxic. Second, graphite nanoparticles exhibited a negative impact on glioblastoma, but not on hepatoma cells. The studied carbon nanoparticles could be a potentially useful tool for therapeutics delivery to the brain tissue with minimal side effects on the hepatocytes. Furthermore, we showed the influence of the nanoparticles on the stable, fluorescently labeled tumor cell lines and concluded that the labeled cells are suitable for drug cytotoxicity tests.

No MeSH data available.


Related in: MedlinePlus