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Effects of electroacupuncture on recovery of the electrophysiological properties of the rabbit gastrocnemius after contusion: an in vivo animal study.

Liu S, Wang R, Luo D, Xu Q, Xiao C, Lin P, Yu Z, Zhao X, Cai R, Ma J, Zhang Q, Wang Y - BMC Complement Altern Med (2015)

Bottom Line: We also examined acetylcholinesterase (AchE) and Agrin expression in the neuromuscular junction (NMJ) via immunohistochemistry.Our results showed that EA at the ST36 and Ashi acupoints effectively promoted recovery of the electrophysiological properties of the rabbit GM after contusion.The effects of EA were realized by promotion of the regeneration of myofibers and nerve fibers, as well as acceleration of NMJ reconstruction by upregulation of AchE and Agrin expression in the motor endplate area.

View Article: PubMed Central - PubMed

Affiliation: Department of Graduate School, Beijing University of Chinese Medicine, No. 11, North 3rd Ring East Road, Chaoyang District, Beijing, 100029, China. liu200578@163.com.

ABSTRACT

Background: Our preliminary studies indicated that electroacupuncture (EA) at the ST36 and Ashi acupoints could promote regeneration of the rabbit gastrocnemius (GM) by improving microcirculation perfusion, promoting the recovery of myofiber structures, and inhibiting excessive fibrosis. However, the effects of EA on recovery of the electrophysiological properties of the GM after contusion are not yet clear. Thus, the purpose of this study was to investigate the effects of EA at the Zusanli (ST36) and Ashi acupoints with regard to recovery of the electrophysiological properties of the rabbit GM after contusion.

Methods: Forty-five rabbits were randomly divided into three groups: normal, contusion, and EA. After an acute GM contusion was produced (in rabbits in the contusion and EA groups), rabbits in the EA group were treated with electrostimulation at the ST36 and Ashi acupoints with 0.4 mA (2 Hz) for 15 min. The contusion group received no EA treatment. At different time points (7, 14, and 28 days) after contusion, we performed surface electromyography (EMG) and measured the nerve conduction velocity (NCV) of the GM and the GM branch of the tibial nerve. We also examined acetylcholinesterase (AchE) and Agrin expression in the neuromuscular junction (NMJ) via immunohistochemistry.

Results: Compared with the contusion group, the EMG amplitude and NCV in rabbits in the EA group were significantly higher at all time points after contusion. AchE and Agrin expression in the EA group were significantly higher than those in the contusion group.

Conclusions: Our results showed that EA at the ST36 and Ashi acupoints effectively promoted recovery of the electrophysiological properties of the rabbit GM after contusion. The effects of EA were realized by promotion of the regeneration of myofibers and nerve fibers, as well as acceleration of NMJ reconstruction by upregulation of AchE and Agrin expression in the motor endplate area.

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Comparison of NCVs at different time points after contusion. (a) NCVs under the condition of fixed transmission distance. The red wave represents electrode 1, and the blue wave represents electrode 2). (b) NCVs at days 7, 14, and 28 in the EA, contusion, and control groups. Contusion versus normal, ##P < 0.01; EA versus contusion, ▲▲P < 0.01; EA versus normal, *P < 0.05, **P < 0.01 (n = 5).
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Fig3: Comparison of NCVs at different time points after contusion. (a) NCVs under the condition of fixed transmission distance. The red wave represents electrode 1, and the blue wave represents electrode 2). (b) NCVs at days 7, 14, and 28 in the EA, contusion, and control groups. Contusion versus normal, ##P < 0.01; EA versus contusion, ▲▲P < 0.01; EA versus normal, *P < 0.05, **P < 0.01 (n = 5).

Mentions: NCV can be used to detect nerve conduction in motor neurons that innervate the GM, reflecting the integrity of neurons and myelin [11]. GM injury is accompanied by nerve injury; therefore, when myofiber regeneration occurs, neurons also gradually regenerate and make contact with myofibers. Additionally, NCV acceleration occurs. In our analysis, as the time after contusion increased, the NCV of the contusion group remained slower than that of the normal group (F =93.01, P < 0.01; F =14.08, P =0.006, and F =22.37, P =0.001,). The nerve conduction time (ms) gradually shortened (i.e., accelerated) in the EA and contusion groups, while NCV gradually increased. Furthermore, the NCV of the EA group was significantly faster than that of the contusion group on day 28 (F =17.20, P <0.01), but slower than that of the normal group, although the difference was not statistically significant (F =2.54, P =0.15; Figure 3a and b).Figure 3


Effects of electroacupuncture on recovery of the electrophysiological properties of the rabbit gastrocnemius after contusion: an in vivo animal study.

Liu S, Wang R, Luo D, Xu Q, Xiao C, Lin P, Yu Z, Zhao X, Cai R, Ma J, Zhang Q, Wang Y - BMC Complement Altern Med (2015)

Comparison of NCVs at different time points after contusion. (a) NCVs under the condition of fixed transmission distance. The red wave represents electrode 1, and the blue wave represents electrode 2). (b) NCVs at days 7, 14, and 28 in the EA, contusion, and control groups. Contusion versus normal, ##P < 0.01; EA versus contusion, ▲▲P < 0.01; EA versus normal, *P < 0.05, **P < 0.01 (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4376503&req=5

Fig3: Comparison of NCVs at different time points after contusion. (a) NCVs under the condition of fixed transmission distance. The red wave represents electrode 1, and the blue wave represents electrode 2). (b) NCVs at days 7, 14, and 28 in the EA, contusion, and control groups. Contusion versus normal, ##P < 0.01; EA versus contusion, ▲▲P < 0.01; EA versus normal, *P < 0.05, **P < 0.01 (n = 5).
Mentions: NCV can be used to detect nerve conduction in motor neurons that innervate the GM, reflecting the integrity of neurons and myelin [11]. GM injury is accompanied by nerve injury; therefore, when myofiber regeneration occurs, neurons also gradually regenerate and make contact with myofibers. Additionally, NCV acceleration occurs. In our analysis, as the time after contusion increased, the NCV of the contusion group remained slower than that of the normal group (F =93.01, P < 0.01; F =14.08, P =0.006, and F =22.37, P =0.001,). The nerve conduction time (ms) gradually shortened (i.e., accelerated) in the EA and contusion groups, while NCV gradually increased. Furthermore, the NCV of the EA group was significantly faster than that of the contusion group on day 28 (F =17.20, P <0.01), but slower than that of the normal group, although the difference was not statistically significant (F =2.54, P =0.15; Figure 3a and b).Figure 3

Bottom Line: We also examined acetylcholinesterase (AchE) and Agrin expression in the neuromuscular junction (NMJ) via immunohistochemistry.Our results showed that EA at the ST36 and Ashi acupoints effectively promoted recovery of the electrophysiological properties of the rabbit GM after contusion.The effects of EA were realized by promotion of the regeneration of myofibers and nerve fibers, as well as acceleration of NMJ reconstruction by upregulation of AchE and Agrin expression in the motor endplate area.

View Article: PubMed Central - PubMed

Affiliation: Department of Graduate School, Beijing University of Chinese Medicine, No. 11, North 3rd Ring East Road, Chaoyang District, Beijing, 100029, China. liu200578@163.com.

ABSTRACT

Background: Our preliminary studies indicated that electroacupuncture (EA) at the ST36 and Ashi acupoints could promote regeneration of the rabbit gastrocnemius (GM) by improving microcirculation perfusion, promoting the recovery of myofiber structures, and inhibiting excessive fibrosis. However, the effects of EA on recovery of the electrophysiological properties of the GM after contusion are not yet clear. Thus, the purpose of this study was to investigate the effects of EA at the Zusanli (ST36) and Ashi acupoints with regard to recovery of the electrophysiological properties of the rabbit GM after contusion.

Methods: Forty-five rabbits were randomly divided into three groups: normal, contusion, and EA. After an acute GM contusion was produced (in rabbits in the contusion and EA groups), rabbits in the EA group were treated with electrostimulation at the ST36 and Ashi acupoints with 0.4 mA (2 Hz) for 15 min. The contusion group received no EA treatment. At different time points (7, 14, and 28 days) after contusion, we performed surface electromyography (EMG) and measured the nerve conduction velocity (NCV) of the GM and the GM branch of the tibial nerve. We also examined acetylcholinesterase (AchE) and Agrin expression in the neuromuscular junction (NMJ) via immunohistochemistry.

Results: Compared with the contusion group, the EMG amplitude and NCV in rabbits in the EA group were significantly higher at all time points after contusion. AchE and Agrin expression in the EA group were significantly higher than those in the contusion group.

Conclusions: Our results showed that EA at the ST36 and Ashi acupoints effectively promoted recovery of the electrophysiological properties of the rabbit GM after contusion. The effects of EA were realized by promotion of the regeneration of myofibers and nerve fibers, as well as acceleration of NMJ reconstruction by upregulation of AchE and Agrin expression in the motor endplate area.

Show MeSH
Related in: MedlinePlus