Limits...
Overlapping dose responses of spermatogenic and extragonadal testosterone actions jeopardize the principle of hormonal male contraception.

Oduwole OO, Vydra N, Wood NE, Samanta L, Owen L, Keevil B, Donaldson M, Naresh K, Huhtaniemi IT - FASEB J. (2014)

Bottom Line: Testosterone (T), alone or in combination with progestin, provides a promising approach to hormonal male contraception.A serious drawback of the treatment is that a significant proportion of men do not reach azoospermia or severe oligozoospermia, commensurate with contraceptive efficacy.Conspicuously, all dose responses to T were practically superimposable, and no dose of T could be defined that would maintain sexual function and suppress gonadotropins without simultaneously activating spermatogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer, and.

Show MeSH

Related in: MedlinePlus

Representative histological images of the testis in WT mice (A), LHR−/− mice (B), and LHR−/− mice treated with 1.5 mg T (C), 2.5 mg T (D), 5.0 mg T (E), and T Silastic implant (F), after 90 d of treatment. Elongated spermatids are clearly visible in the tubules of WT (A), 5.0 mg T (E), and T Silastic implant-treated (F) mice. Arrows (D) indicate elongated spermatids in a few seminiferous tubules of the 2.5 mg T-treated mice. Absence of Leydig cells between seminiferous tubules is evident in LHR−/− and LHR−/− T-treated mice. Scale bars = 50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4376501&req=5

Figure 4: Representative histological images of the testis in WT mice (A), LHR−/− mice (B), and LHR−/− mice treated with 1.5 mg T (C), 2.5 mg T (D), 5.0 mg T (E), and T Silastic implant (F), after 90 d of treatment. Elongated spermatids are clearly visible in the tubules of WT (A), 5.0 mg T (E), and T Silastic implant-treated (F) mice. Arrows (D) indicate elongated spermatids in a few seminiferous tubules of the 2.5 mg T-treated mice. Absence of Leydig cells between seminiferous tubules is evident in LHR−/− and LHR−/− T-treated mice. Scale bars = 50 μm.

Mentions: The H&E staining of the LHR−/− mouse testes (Fig. 4B) and those of the mice treated with 0.5 mg T (data not shown) and 1.5 mg T (Fig. 4C) revealed similar cellular architecture. They were marked by narrow seminiferous tubules (Table 1), decreased number and size of Leydig cells, and arrested spermatogenesis at the round spermatid stage, as compared with WT testes (Fig. 4A), which showed normal spermatogenesis and formation of seminiferous tubular lumen surrounded by mature elongated spermatids. In mice treated with 2.5 mg T (Fig. 4D), progression of spermatogenesis to elongated spermatids was present in a few seminiferous tubules per section of testis (arrowed). In mice treated with 5.0 mg T (Fig. 4E) and the Silastic T implants (Fig. 4F), numerous elongated spermatids, indistinguishable from the WT testes, were observed in all seminiferous tubule sections.


Overlapping dose responses of spermatogenic and extragonadal testosterone actions jeopardize the principle of hormonal male contraception.

Oduwole OO, Vydra N, Wood NE, Samanta L, Owen L, Keevil B, Donaldson M, Naresh K, Huhtaniemi IT - FASEB J. (2014)

Representative histological images of the testis in WT mice (A), LHR−/− mice (B), and LHR−/− mice treated with 1.5 mg T (C), 2.5 mg T (D), 5.0 mg T (E), and T Silastic implant (F), after 90 d of treatment. Elongated spermatids are clearly visible in the tubules of WT (A), 5.0 mg T (E), and T Silastic implant-treated (F) mice. Arrows (D) indicate elongated spermatids in a few seminiferous tubules of the 2.5 mg T-treated mice. Absence of Leydig cells between seminiferous tubules is evident in LHR−/− and LHR−/− T-treated mice. Scale bars = 50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376501&req=5

Figure 4: Representative histological images of the testis in WT mice (A), LHR−/− mice (B), and LHR−/− mice treated with 1.5 mg T (C), 2.5 mg T (D), 5.0 mg T (E), and T Silastic implant (F), after 90 d of treatment. Elongated spermatids are clearly visible in the tubules of WT (A), 5.0 mg T (E), and T Silastic implant-treated (F) mice. Arrows (D) indicate elongated spermatids in a few seminiferous tubules of the 2.5 mg T-treated mice. Absence of Leydig cells between seminiferous tubules is evident in LHR−/− and LHR−/− T-treated mice. Scale bars = 50 μm.
Mentions: The H&E staining of the LHR−/− mouse testes (Fig. 4B) and those of the mice treated with 0.5 mg T (data not shown) and 1.5 mg T (Fig. 4C) revealed similar cellular architecture. They were marked by narrow seminiferous tubules (Table 1), decreased number and size of Leydig cells, and arrested spermatogenesis at the round spermatid stage, as compared with WT testes (Fig. 4A), which showed normal spermatogenesis and formation of seminiferous tubular lumen surrounded by mature elongated spermatids. In mice treated with 2.5 mg T (Fig. 4D), progression of spermatogenesis to elongated spermatids was present in a few seminiferous tubules per section of testis (arrowed). In mice treated with 5.0 mg T (Fig. 4E) and the Silastic T implants (Fig. 4F), numerous elongated spermatids, indistinguishable from the WT testes, were observed in all seminiferous tubule sections.

Bottom Line: Testosterone (T), alone or in combination with progestin, provides a promising approach to hormonal male contraception.A serious drawback of the treatment is that a significant proportion of men do not reach azoospermia or severe oligozoospermia, commensurate with contraceptive efficacy.Conspicuously, all dose responses to T were practically superimposable, and no dose of T could be defined that would maintain sexual function and suppress gonadotropins without simultaneously activating spermatogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer, and.

Show MeSH
Related in: MedlinePlus