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Distinctive expression patterns of glycoprotein non-metastatic B and folliculin in renal tumors in patients with Birt-Hogg-Dubé syndrome.

Furuya M, Hong SB, Tanaka R, Kuroda N, Nagashima Y, Nagahama K, Suyama T, Yao M, Nakatani Y - Cancer Sci. (2015)

Bottom Line: Western blot and immunostaining analyses revealed that FLCN-related RCCs showed overexpression of GPNMB and underexpression of FLCN, whereas sporadic tumors showed inverted patterns.GPNMB mRNA in FLCN-related RCCs was 23-fold more abundant than in sporadic tumors.The distinctive expression patterns of GPNMB and FLCN might identify patients with RCCs who need further work-up for BHD.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.

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Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
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fig03: Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).

Mentions: Next, we investigated FLCN protein expression. Using the antibody D14G9 for Western blotting, the FLCN bands (70 kDa) stained strongly in non-tumor regions of BHD kidneys, whereas FLCN bands were either undetectable or much weaker in RCCs of BHD patients, compared with the corresponding non-tumor parts (Fig.3a). In sporadic tumors, the FLCN bands were clearly detected (Fig.3b). We also obtained similar results using antibodies sc-25168 and ab93196 (data not shown).


Distinctive expression patterns of glycoprotein non-metastatic B and folliculin in renal tumors in patients with Birt-Hogg-Dubé syndrome.

Furuya M, Hong SB, Tanaka R, Kuroda N, Nagashima Y, Nagahama K, Suyama T, Yao M, Nakatani Y - Cancer Sci. (2015)

Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376441&req=5

fig03: Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
Mentions: Next, we investigated FLCN protein expression. Using the antibody D14G9 for Western blotting, the FLCN bands (70 kDa) stained strongly in non-tumor regions of BHD kidneys, whereas FLCN bands were either undetectable or much weaker in RCCs of BHD patients, compared with the corresponding non-tumor parts (Fig.3a). In sporadic tumors, the FLCN bands were clearly detected (Fig.3b). We also obtained similar results using antibodies sc-25168 and ab93196 (data not shown).

Bottom Line: Western blot and immunostaining analyses revealed that FLCN-related RCCs showed overexpression of GPNMB and underexpression of FLCN, whereas sporadic tumors showed inverted patterns.GPNMB mRNA in FLCN-related RCCs was 23-fold more abundant than in sporadic tumors.The distinctive expression patterns of GPNMB and FLCN might identify patients with RCCs who need further work-up for BHD.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.

Show MeSH
Related in: MedlinePlus