Limits...
Distinctive expression patterns of glycoprotein non-metastatic B and folliculin in renal tumors in patients with Birt-Hogg-Dubé syndrome.

Furuya M, Hong SB, Tanaka R, Kuroda N, Nagashima Y, Nagahama K, Suyama T, Yao M, Nakatani Y - Cancer Sci. (2015)

Bottom Line: Birt-Hogg-Dubé syndrome (BHD) is an inherited disorder associated with a germline mutation of the folliculin gene (FLCN).Histopathological analysis revealed that the most frequent histological type was chromophobe RCC (n = 12), followed by hybrid oncocytic/chromophobe tumor (n = 6).Somatic mutation analysis revealed small intragenic mutations in six cases and loss of heterozygosity in two cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.

Show MeSH

Related in: MedlinePlus

Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4376441&req=5

fig03: Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).

Mentions: Next, we investigated FLCN protein expression. Using the antibody D14G9 for Western blotting, the FLCN bands (70 kDa) stained strongly in non-tumor regions of BHD kidneys, whereas FLCN bands were either undetectable or much weaker in RCCs of BHD patients, compared with the corresponding non-tumor parts (Fig.3a). In sporadic tumors, the FLCN bands were clearly detected (Fig.3b). We also obtained similar results using antibodies sc-25168 and ab93196 (data not shown).


Distinctive expression patterns of glycoprotein non-metastatic B and folliculin in renal tumors in patients with Birt-Hogg-Dubé syndrome.

Furuya M, Hong SB, Tanaka R, Kuroda N, Nagashima Y, Nagahama K, Suyama T, Yao M, Nakatani Y - Cancer Sci. (2015)

Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376441&req=5

fig03: Folliculin (FLCN) expression in Birt–Hogg–Dubé (BHD) and sporadic tumors. (a) Representative results of Western blot analysis of BHD kidneys. Patients BHD9 and BHD17 had two independent tumors (T1, T2). The FLCN bands (70 kDa) were clearly detected in non-tumor lanes but barely seen in tumor lanes. N, normal-looking region; T, tumor region. (b) Representative results of Western blotting analysis of sporadic renal tumors and normal kidneys without the background of BHD. Sporadic renal tumors include clear cell renal cell carcinomas (RCCs) (n = 3), oncocytoma (n = 1), papillary RCC (n = 1), and chromophobe RCCs (n = 3).
Mentions: Next, we investigated FLCN protein expression. Using the antibody D14G9 for Western blotting, the FLCN bands (70 kDa) stained strongly in non-tumor regions of BHD kidneys, whereas FLCN bands were either undetectable or much weaker in RCCs of BHD patients, compared with the corresponding non-tumor parts (Fig.3a). In sporadic tumors, the FLCN bands were clearly detected (Fig.3b). We also obtained similar results using antibodies sc-25168 and ab93196 (data not shown).

Bottom Line: Birt-Hogg-Dubé syndrome (BHD) is an inherited disorder associated with a germline mutation of the folliculin gene (FLCN).Histopathological analysis revealed that the most frequent histological type was chromophobe RCC (n = 12), followed by hybrid oncocytic/chromophobe tumor (n = 6).Somatic mutation analysis revealed small intragenic mutations in six cases and loss of heterozygosity in two cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.

Show MeSH
Related in: MedlinePlus