Distinctive expression patterns of glycoprotein non-metastatic B and folliculin in renal tumors in patients with Birt-Hogg-Dubé syndrome.
Bottom Line: Western blot and immunostaining analyses revealed that FLCN-related RCCs showed overexpression of GPNMB and underexpression of FLCN, whereas sporadic tumors showed inverted patterns.GPNMB mRNA in FLCN-related RCCs was 23-fold more abundant than in sporadic tumors.The distinctive expression patterns of GPNMB and FLCN might identify patients with RCCs who need further work-up for BHD.
Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.Show MeSH
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Mentions: Next, we investigated FLCN protein expression. Using the antibody D14G9 for Western blotting, the FLCN bands (70 kDa) stained strongly in non-tumor regions of BHD kidneys, whereas FLCN bands were either undetectable or much weaker in RCCs of BHD patients, compared with the corresponding non-tumor parts (Fig.3a). In sporadic tumors, the FLCN bands were clearly detected (Fig.3b). We also obtained similar results using antibodies sc-25168 and ab93196 (data not shown).
Affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.