Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation.
Bottom Line: High PES1 expression was associated with worse overall and relapse-free survival.This growth inhibition was associated with the expression of NB differentiation markers.These data suggest that PES1 is a marker of NB outcome, that it regulates NB cell proliferation, and is associated with NB differentiation.
Affiliation: Department of Pathology and Laboratory Medicine, Nagoya University Hospital, Nagoya, Japan.Show MeSH
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Mentions: We then examined whether the induction of differentiation can change the PES1 expression. Historically, various methods have been used to induce NB differentiation, including serum starvation and treatment with all-trans retinoic acid (ATRA).28–31 A derivative of vitamin A, ATRA is essential for development and differentiation. All-trans retinoic acid-induced differentiation of leukemic cells has been shown to dramatically improve the prognosis of patients with acute promyelocytic leukemia.32 In addition, ATRA can differentiate NB cells. We induced differentiation in NB cell lines with serum reduction (from 10% FBS in original culture to 1%) and ATRA (1 μM). This treatment increased the number and length of the neuritic extensions of NB39 and TNB1 cells (Fig.4a, Fig. S2), and these neurites were positive for Tuj1, a neuronal marker. This differentiation induction was further verified by the increased expression of a differentiation-related gene (Fig.4b, Fig. S2).33 Interestingly, the differentiation induction decreased PES1 expression (Fig.4b, Fig. S2). This result suggests the possibility that ATRA-induced differentiation modulates PES1 expression.
Affiliation: Department of Pathology and Laboratory Medicine, Nagoya University Hospital, Nagoya, Japan.