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Full-thickness cartilage defects are repaired via a microfracture technique and intraarticular injection of the small-molecule compound kartogenin.

Xu X, Shi D, Shen Y, Xu Z, Dai J, Chen D, Teng H, Jiang Q - Arthritis Res. Ther. (2015)

Bottom Line: At 4 weeks, group 1 showed better defect filling and a greater number of chondrocyte-like cells compared with group 2.At 12 weeks, group 1 showed statistically significantly higher ICRS scores and modified O'Driscoll scores compared with group 2.More hyaline cartilage-like tissue was found in the defects of group 1 at 12 weeks.

View Article: PubMed Central - PubMed

Affiliation: The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital, Medical School, Nanjing University, Zhongshan Road 321, Nanjing, 210008, Jiangsu, China. xuxingquan12345@163.com.

ABSTRACT

Introduction: Microfracture does not properly repair full-thickness cartilage defects. The purpose of this study was to evaluate the effect of intraarticular injection of the small-molecule compound kartogenin (KGN) on the restoration of a full-thickness cartilage defect treated with microfracture in a rabbit model.

Methods: Full-thickness cartilage defects (3.5 mm in diameter and 3 mm in depth) were created in the patellar groove of the right femurs of 24 female New Zealand White rabbits. The rabbits were divided into two groups (12 in each group) based on postsurgery treatment differences, as follows: microfracture plus weekly intraarticular injection of KGN (group 1) and microfracture plus dimethyl sulfoxide (group 2). Six rabbits from each group were illed at 4 and 12 weeks after surgery, and their knees were harvested. The outcome was assessed both macroscopically, by using the International Cartilage Repair Society (ICRS) macroscopic evaluation system, and histologically, by using the modified O'Driscoll histologic scoring system. Immunohistochemistry for type II and I collagen was also conducted.

Results: At 4 weeks, group 1 showed better defect filling and a greater number of chondrocyte-like cells compared with group 2. At 12 weeks, group 1 showed statistically significantly higher ICRS scores and modified O'Driscoll scores compared with group 2. More hyaline cartilage-like tissue was found in the defects of group 1 at 12 weeks.

Conclusions: Intraarticular injection of KGN enhances the quality of full-thickness cartilage defects repair after microfracture, with better defect filling and increased hyaline-like cartilage formation.

No MeSH data available.


Related in: MedlinePlus

Microfractured cartilage defect (A) and macroscopic appearance of the specimens in groups 1 (B, D) and 2 (C, E). Gross appearance was shown at 4 (B, C) and 12 weeks (D, E). (B) The repair tissue covered more than 50% of the cartilage defect. (C) Little repair tissue was observed in the cartilage defect. (D) Repair tissue almost completely covered the defect in the experimental group, but the defect in the control group (E) was insufficiently repaired.
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Fig1: Microfractured cartilage defect (A) and macroscopic appearance of the specimens in groups 1 (B, D) and 2 (C, E). Gross appearance was shown at 4 (B, C) and 12 weeks (D, E). (B) The repair tissue covered more than 50% of the cartilage defect. (C) Little repair tissue was observed in the cartilage defect. (D) Repair tissue almost completely covered the defect in the experimental group, but the defect in the control group (E) was insufficiently repaired.

Mentions: Each rabbit was anesthetized with an intramuscular injection of 5 mg droperidol and 0.1 g ketamine. In addition, anesthetization was maintained by slowly administering ketamine and diazepam through an auricular vein during the operation. The rabbit was placed in the supine position, and a medial parapatellar approach was used to operate on the right knee joint. Then, the patella was dislocated laterally to expose the articular surface. A full-thickness osteochondral defect (3.5 mm in diameter and 3 mm in depth) was made in the center of the trochlear groove by using an osteoarticular transplantation system. Then microfracture was performed with a 0.35 (0.9 mm) Kirschner wire (Figure 1A).Figure 1


Full-thickness cartilage defects are repaired via a microfracture technique and intraarticular injection of the small-molecule compound kartogenin.

Xu X, Shi D, Shen Y, Xu Z, Dai J, Chen D, Teng H, Jiang Q - Arthritis Res. Ther. (2015)

Microfractured cartilage defect (A) and macroscopic appearance of the specimens in groups 1 (B, D) and 2 (C, E). Gross appearance was shown at 4 (B, C) and 12 weeks (D, E). (B) The repair tissue covered more than 50% of the cartilage defect. (C) Little repair tissue was observed in the cartilage defect. (D) Repair tissue almost completely covered the defect in the experimental group, but the defect in the control group (E) was insufficiently repaired.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4376363&req=5

Fig1: Microfractured cartilage defect (A) and macroscopic appearance of the specimens in groups 1 (B, D) and 2 (C, E). Gross appearance was shown at 4 (B, C) and 12 weeks (D, E). (B) The repair tissue covered more than 50% of the cartilage defect. (C) Little repair tissue was observed in the cartilage defect. (D) Repair tissue almost completely covered the defect in the experimental group, but the defect in the control group (E) was insufficiently repaired.
Mentions: Each rabbit was anesthetized with an intramuscular injection of 5 mg droperidol and 0.1 g ketamine. In addition, anesthetization was maintained by slowly administering ketamine and diazepam through an auricular vein during the operation. The rabbit was placed in the supine position, and a medial parapatellar approach was used to operate on the right knee joint. Then, the patella was dislocated laterally to expose the articular surface. A full-thickness osteochondral defect (3.5 mm in diameter and 3 mm in depth) was made in the center of the trochlear groove by using an osteoarticular transplantation system. Then microfracture was performed with a 0.35 (0.9 mm) Kirschner wire (Figure 1A).Figure 1

Bottom Line: At 4 weeks, group 1 showed better defect filling and a greater number of chondrocyte-like cells compared with group 2.At 12 weeks, group 1 showed statistically significantly higher ICRS scores and modified O'Driscoll scores compared with group 2.More hyaline cartilage-like tissue was found in the defects of group 1 at 12 weeks.

View Article: PubMed Central - PubMed

Affiliation: The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital, Medical School, Nanjing University, Zhongshan Road 321, Nanjing, 210008, Jiangsu, China. xuxingquan12345@163.com.

ABSTRACT

Introduction: Microfracture does not properly repair full-thickness cartilage defects. The purpose of this study was to evaluate the effect of intraarticular injection of the small-molecule compound kartogenin (KGN) on the restoration of a full-thickness cartilage defect treated with microfracture in a rabbit model.

Methods: Full-thickness cartilage defects (3.5 mm in diameter and 3 mm in depth) were created in the patellar groove of the right femurs of 24 female New Zealand White rabbits. The rabbits were divided into two groups (12 in each group) based on postsurgery treatment differences, as follows: microfracture plus weekly intraarticular injection of KGN (group 1) and microfracture plus dimethyl sulfoxide (group 2). Six rabbits from each group were illed at 4 and 12 weeks after surgery, and their knees were harvested. The outcome was assessed both macroscopically, by using the International Cartilage Repair Society (ICRS) macroscopic evaluation system, and histologically, by using the modified O'Driscoll histologic scoring system. Immunohistochemistry for type II and I collagen was also conducted.

Results: At 4 weeks, group 1 showed better defect filling and a greater number of chondrocyte-like cells compared with group 2. At 12 weeks, group 1 showed statistically significantly higher ICRS scores and modified O'Driscoll scores compared with group 2. More hyaline cartilage-like tissue was found in the defects of group 1 at 12 weeks.

Conclusions: Intraarticular injection of KGN enhances the quality of full-thickness cartilage defects repair after microfracture, with better defect filling and increased hyaline-like cartilage formation.

No MeSH data available.


Related in: MedlinePlus