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Free-radical Destruction of Sphingolipids Resulting in 2-hexadecenal Formation.

Shadyro O, Lisovskaya A, Semenkova G, Edimecheva I, Amaegberi N - Lipid Insights (2015)

Bottom Line: These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity.Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex.This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Belarusian State University, Minsk, Belarus.

ABSTRACT
The action of hypochlorous acid (HOCl) and γ-radiation on aqueous lysosphingolipid dispersions was found to produce 2-hexadecenal (Hex). This process includes the stages of formation of nitrogen-centered radicals from the starting molecules and the subsequent fragmentation of these radicals via the rupture of C-C and O-H bonds. These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity. Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex. This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms.

No MeSH data available.


Related in: MedlinePlus

Accumulation of Hex in deaerated aqueous dispersions of sphingolipids as function of HOCl concentration added. (A) 5 mM SPH (50 mM PBS pH 5, 1 mM SDS). (B) 2 mM SPC (50 mM PBS, pH 5). (C) 5 mM S1P (50 mM PBS, pH 7.4, 1 mM SDS). Error bars indicate SD of the means (n ≥ 3).
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f3-lpi-8-2015-001: Accumulation of Hex in deaerated aqueous dispersions of sphingolipids as function of HOCl concentration added. (A) 5 mM SPH (50 mM PBS pH 5, 1 mM SDS). (B) 2 mM SPC (50 mM PBS, pH 5). (C) 5 mM S1P (50 mM PBS, pH 7.4, 1 mM SDS). Error bars indicate SD of the means (n ≥ 3).

Mentions: Moreover, the introduction of HOCl into SPH, S1P, and SPC dispersions resulted in the formation of Hex in amounts varying with the reagent concentration, as shown in Figure 3.


Free-radical Destruction of Sphingolipids Resulting in 2-hexadecenal Formation.

Shadyro O, Lisovskaya A, Semenkova G, Edimecheva I, Amaegberi N - Lipid Insights (2015)

Accumulation of Hex in deaerated aqueous dispersions of sphingolipids as function of HOCl concentration added. (A) 5 mM SPH (50 mM PBS pH 5, 1 mM SDS). (B) 2 mM SPC (50 mM PBS, pH 5). (C) 5 mM S1P (50 mM PBS, pH 7.4, 1 mM SDS). Error bars indicate SD of the means (n ≥ 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4376205&req=5

f3-lpi-8-2015-001: Accumulation of Hex in deaerated aqueous dispersions of sphingolipids as function of HOCl concentration added. (A) 5 mM SPH (50 mM PBS pH 5, 1 mM SDS). (B) 2 mM SPC (50 mM PBS, pH 5). (C) 5 mM S1P (50 mM PBS, pH 7.4, 1 mM SDS). Error bars indicate SD of the means (n ≥ 3).
Mentions: Moreover, the introduction of HOCl into SPH, S1P, and SPC dispersions resulted in the formation of Hex in amounts varying with the reagent concentration, as shown in Figure 3.

Bottom Line: These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity.Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex.This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Belarusian State University, Minsk, Belarus.

ABSTRACT
The action of hypochlorous acid (HOCl) and γ-radiation on aqueous lysosphingolipid dispersions was found to produce 2-hexadecenal (Hex). This process includes the stages of formation of nitrogen-centered radicals from the starting molecules and the subsequent fragmentation of these radicals via the rupture of C-C and O-H bonds. These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity. Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex. This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms.

No MeSH data available.


Related in: MedlinePlus