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Electrically induced limbic seizures: preliminary findings in a rodent model.

Kowski AB, Holtkamp M - J Exp Neurosci (2015)

Bottom Line: In epilepsy, novel pharmacological and nonpharmacological treatment approaches are commonly assessed in model systems of acute motor and often generalized seizures.Limbic structures play a major role in human intractable epilepsy.This model may represent a reliable screening tool for new treatment approaches such as deep brain stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Epilepsy-Center Berlin-Brandenburg, Charité-Universitätsmedizin Berlin, Berlin, Germany.

ABSTRACT
In epilepsy, novel pharmacological and nonpharmacological treatment approaches are commonly assessed in model systems of acute motor and often generalized seizures. We developed a rodent model with short-term electrical stimulation of the perforant path resulting in stereotyped limbic seizures. Limbic structures play a major role in human intractable epilepsy. In 10 rats, single electrical 5-second and 20-Hz stimuli to the perforant path reliably produced limbic seizures characterized by resting behavior and subtle motor signs. Electrophysiological recordings from the dentate gyrus demonstrated a seizure pattern with 4-Hz to 5-Hz discharges. Multiple inductions of seizures within 72 hours did not alter behavioral and electrophysiological seizure characteristics. Electrophysiological excitatory and inhibitory parameters assessed by evoked single and paired pulses did not change with increasing number of seizures. We present preliminary findings on a new model of electrically induced limbic seizures of mesiotemporal origin. This model may represent a reliable screening tool for new treatment approaches such as deep brain stimulation.

No MeSH data available.


Related in: MedlinePlus

Experimental protocol.Notes: Seven days after implantation of electrodes, threshold for eliciting a maximum population spike (PS) response was assessed. Over a time course of 72 hours, a total of six seizures were induced. Before the first, fifth, and sixth seizures, dentate gyrus excitability was assessed by single and paired pulses.
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f1-jen-9-2015-007: Experimental protocol.Notes: Seven days after implantation of electrodes, threshold for eliciting a maximum population spike (PS) response was assessed. Over a time course of 72 hours, a total of six seizures were induced. Before the first, fifth, and sixth seizures, dentate gyrus excitability was assessed by single and paired pulses.

Mentions: After implantation of electrodes into limbic structures, animals were allowed to recover for 1 week before experiments were started. Before the first induced seizure, single and paired pulses were applied in order to test for neuronal excitability. The delays between seizure induction and the time points for single and paired pulse measurements are demonstrated as timeline in Figure 1. Experiments were conducted in accordance with the German Animal Protection Act and had been approved by the regional authority (“LAGeSo—Landesamt für Gesundheit und Soziales”).


Electrically induced limbic seizures: preliminary findings in a rodent model.

Kowski AB, Holtkamp M - J Exp Neurosci (2015)

Experimental protocol.Notes: Seven days after implantation of electrodes, threshold for eliciting a maximum population spike (PS) response was assessed. Over a time course of 72 hours, a total of six seizures were induced. Before the first, fifth, and sixth seizures, dentate gyrus excitability was assessed by single and paired pulses.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4376203&req=5

f1-jen-9-2015-007: Experimental protocol.Notes: Seven days after implantation of electrodes, threshold for eliciting a maximum population spike (PS) response was assessed. Over a time course of 72 hours, a total of six seizures were induced. Before the first, fifth, and sixth seizures, dentate gyrus excitability was assessed by single and paired pulses.
Mentions: After implantation of electrodes into limbic structures, animals were allowed to recover for 1 week before experiments were started. Before the first induced seizure, single and paired pulses were applied in order to test for neuronal excitability. The delays between seizure induction and the time points for single and paired pulse measurements are demonstrated as timeline in Figure 1. Experiments were conducted in accordance with the German Animal Protection Act and had been approved by the regional authority (“LAGeSo—Landesamt für Gesundheit und Soziales”).

Bottom Line: In epilepsy, novel pharmacological and nonpharmacological treatment approaches are commonly assessed in model systems of acute motor and often generalized seizures.Limbic structures play a major role in human intractable epilepsy.This model may represent a reliable screening tool for new treatment approaches such as deep brain stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Epilepsy-Center Berlin-Brandenburg, Charité-Universitätsmedizin Berlin, Berlin, Germany.

ABSTRACT
In epilepsy, novel pharmacological and nonpharmacological treatment approaches are commonly assessed in model systems of acute motor and often generalized seizures. We developed a rodent model with short-term electrical stimulation of the perforant path resulting in stereotyped limbic seizures. Limbic structures play a major role in human intractable epilepsy. In 10 rats, single electrical 5-second and 20-Hz stimuli to the perforant path reliably produced limbic seizures characterized by resting behavior and subtle motor signs. Electrophysiological recordings from the dentate gyrus demonstrated a seizure pattern with 4-Hz to 5-Hz discharges. Multiple inductions of seizures within 72 hours did not alter behavioral and electrophysiological seizure characteristics. Electrophysiological excitatory and inhibitory parameters assessed by evoked single and paired pulses did not change with increasing number of seizures. We present preliminary findings on a new model of electrically induced limbic seizures of mesiotemporal origin. This model may represent a reliable screening tool for new treatment approaches such as deep brain stimulation.

No MeSH data available.


Related in: MedlinePlus