The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency.
Bottom Line: Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation.L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development.Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.
Affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.Show MeSH
Mentions: The LC-MS/MS analysis resulted in the identification of multiple members of the ubiquitin-dependent proteasome, such as PSMD1, PSMD2, PSMD7, PSMD8, PSMD11, and PSMD14. Of particular interest, PSMD11 was recently reported to be responsible for the increased proteasome activity in hESCs, which is important for the maintenance of pluripotency (Vilchez et al., 2012). The interaction between L1TD1 and PSMD11 was validated with immunoblotting (Figure 3A). Moreover, silencing of L1TD1 resulted in decreased levels of PSMD11 (Figure 3B).
Affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.