Limits...
The association constant of 5',8-cyclo-2'-deoxyguanosine with cytidine.

Capobianco A, Caruso T, Fusco S, Terzidis MA, Masi A, Chatgilialoglu C, Peluso A - Front Chem (2015)

Bottom Line: The association of 5',8-cyclo-2'-deoxyguanosine (cdG), a DNA tandem lesion, with its complementary base cytosine has been studied by voltammetry and NMR in chloroform, using properly silylated derivatives of the two nucleobases for increasing their solubilities.Both voltammetric data and NMR titrations indicated that the Watson-Crick complex of cytidine with cdG is weaker than that with guanosine, the difference being approximately of one order of magnitude between the two association constants.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica e Biologia, Università di Salerno Fisciano, Salerno, Italy.

ABSTRACT
The association of 5',8-cyclo-2'-deoxyguanosine (cdG), a DNA tandem lesion, with its complementary base cytosine has been studied by voltammetry and NMR in chloroform, using properly silylated derivatives of the two nucleobases for increasing their solubilities. Both voltammetric data and NMR titrations indicated that the Watson-Crick complex of cytidine with cdG is weaker than that with guanosine, the difference being approximately of one order of magnitude between the two association constants.

No MeSH data available.


Partial H-NMR (600 MHz) spectra of 3.6 mM ScdG′ at T = 22 C in the presence of 0 (A), 1.7 (B), 4.7 (C), 8.7 (D), 13.0 (E), 25.0 (F), 36.7 (G), and 68.3 (H) mM dC′ in CDCl3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4376000&req=5

Figure 2: Partial H-NMR (600 MHz) spectra of 3.6 mM ScdG′ at T = 22 C in the presence of 0 (A), 1.7 (B), 4.7 (C), 8.7 (D), 13.0 (E), 25.0 (F), 36.7 (G), and 68.3 (H) mM dC′ in CDCl3.

Mentions: In Figure 2 the most interesting region of the NMR spectra of solutions containing dC′ and ScdG′ in different ratios is reported. As the concentration of dC′ increases, the imino N1–H proton shift of ScdG′ increases from 11.5 ppm to 13.7 ppm, indicating that the Watson-Crick complex, which is by far the most stable motif for G:C association (Abo-Riziq et al., 2005) is actually established in CHCl3, as also observed in modified DNA (Huang et al., 2011).


The association constant of 5',8-cyclo-2'-deoxyguanosine with cytidine.

Capobianco A, Caruso T, Fusco S, Terzidis MA, Masi A, Chatgilialoglu C, Peluso A - Front Chem (2015)

Partial H-NMR (600 MHz) spectra of 3.6 mM ScdG′ at T = 22 C in the presence of 0 (A), 1.7 (B), 4.7 (C), 8.7 (D), 13.0 (E), 25.0 (F), 36.7 (G), and 68.3 (H) mM dC′ in CDCl3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4376000&req=5

Figure 2: Partial H-NMR (600 MHz) spectra of 3.6 mM ScdG′ at T = 22 C in the presence of 0 (A), 1.7 (B), 4.7 (C), 8.7 (D), 13.0 (E), 25.0 (F), 36.7 (G), and 68.3 (H) mM dC′ in CDCl3.
Mentions: In Figure 2 the most interesting region of the NMR spectra of solutions containing dC′ and ScdG′ in different ratios is reported. As the concentration of dC′ increases, the imino N1–H proton shift of ScdG′ increases from 11.5 ppm to 13.7 ppm, indicating that the Watson-Crick complex, which is by far the most stable motif for G:C association (Abo-Riziq et al., 2005) is actually established in CHCl3, as also observed in modified DNA (Huang et al., 2011).

Bottom Line: The association of 5',8-cyclo-2'-deoxyguanosine (cdG), a DNA tandem lesion, with its complementary base cytosine has been studied by voltammetry and NMR in chloroform, using properly silylated derivatives of the two nucleobases for increasing their solubilities.Both voltammetric data and NMR titrations indicated that the Watson-Crick complex of cytidine with cdG is weaker than that with guanosine, the difference being approximately of one order of magnitude between the two association constants.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica e Biologia, Università di Salerno Fisciano, Salerno, Italy.

ABSTRACT
The association of 5',8-cyclo-2'-deoxyguanosine (cdG), a DNA tandem lesion, with its complementary base cytosine has been studied by voltammetry and NMR in chloroform, using properly silylated derivatives of the two nucleobases for increasing their solubilities. Both voltammetric data and NMR titrations indicated that the Watson-Crick complex of cytidine with cdG is weaker than that with guanosine, the difference being approximately of one order of magnitude between the two association constants.

No MeSH data available.