Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs.
Bottom Line: Defects in articular cartilage ultimately result in loss of joint function.The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation.The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.
Affiliation: Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.Show MeSH
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Mentions: For long-term observation, we sacrificed the mice 12 months after transplanting hiPSC-derived particles on day 42, harvested the transplanted sites, and subjected them to histological analysis. All six samples showed cartilage hypertrophy, as indicated by the expression of type X collagen (Figure 5). Among these samples, five had portions of the cartilage replaced with bone-like tissue, but a substantial amount of cartilage with a morphology resembling epiphyseal cartilage remained. These results suggest that the hiPSC-derived cells produced using our protocol undergo hypertrophy, although at a very slow rate. There were no signs of teratoma or other tumor formation at any of the transplanted sites.
Affiliation: Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.