Limits...
Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs.

Yamashita A, Morioka M, Yahara Y, Okada M, Kobayashi T, Kuriyama S, Matsuda S, Tsumaki N - Stem Cell Reports (2015)

Bottom Line: Defects in articular cartilage ultimately result in loss of joint function.The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation.The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.

View Article: PubMed Central - PubMed

Affiliation: Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.

Show MeSH

Related in: MedlinePlus

Long-Term Observations of hiPSC-Derived Particles on Day 42 Transplanted into the Subcutaneous Space of SCID MiceMice were sacrificed 12 months after transplantation. Semiserial sections were stained with H&E and safranin O-fast green-iron hematoxylin and immunostained with anti-type X collagen antibodies. Magnified images of the boxed regions are shown in panels to the immediate right. Scale bar, 500 μm (left) and 50 μm (middle and right). See also Table S1.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4375934&req=5

fig5: Long-Term Observations of hiPSC-Derived Particles on Day 42 Transplanted into the Subcutaneous Space of SCID MiceMice were sacrificed 12 months after transplantation. Semiserial sections were stained with H&E and safranin O-fast green-iron hematoxylin and immunostained with anti-type X collagen antibodies. Magnified images of the boxed regions are shown in panels to the immediate right. Scale bar, 500 μm (left) and 50 μm (middle and right). See also Table S1.

Mentions: For long-term observation, we sacrificed the mice 12 months after transplanting hiPSC-derived particles on day 42, harvested the transplanted sites, and subjected them to histological analysis. All six samples showed cartilage hypertrophy, as indicated by the expression of type X collagen (Figure 5). Among these samples, five had portions of the cartilage replaced with bone-like tissue, but a substantial amount of cartilage with a morphology resembling epiphyseal cartilage remained. These results suggest that the hiPSC-derived cells produced using our protocol undergo hypertrophy, although at a very slow rate. There were no signs of teratoma or other tumor formation at any of the transplanted sites.


Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs.

Yamashita A, Morioka M, Yahara Y, Okada M, Kobayashi T, Kuriyama S, Matsuda S, Tsumaki N - Stem Cell Reports (2015)

Long-Term Observations of hiPSC-Derived Particles on Day 42 Transplanted into the Subcutaneous Space of SCID MiceMice were sacrificed 12 months after transplantation. Semiserial sections were stained with H&E and safranin O-fast green-iron hematoxylin and immunostained with anti-type X collagen antibodies. Magnified images of the boxed regions are shown in panels to the immediate right. Scale bar, 500 μm (left) and 50 μm (middle and right). See also Table S1.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375934&req=5

fig5: Long-Term Observations of hiPSC-Derived Particles on Day 42 Transplanted into the Subcutaneous Space of SCID MiceMice were sacrificed 12 months after transplantation. Semiserial sections were stained with H&E and safranin O-fast green-iron hematoxylin and immunostained with anti-type X collagen antibodies. Magnified images of the boxed regions are shown in panels to the immediate right. Scale bar, 500 μm (left) and 50 μm (middle and right). See also Table S1.
Mentions: For long-term observation, we sacrificed the mice 12 months after transplanting hiPSC-derived particles on day 42, harvested the transplanted sites, and subjected them to histological analysis. All six samples showed cartilage hypertrophy, as indicated by the expression of type X collagen (Figure 5). Among these samples, five had portions of the cartilage replaced with bone-like tissue, but a substantial amount of cartilage with a morphology resembling epiphyseal cartilage remained. These results suggest that the hiPSC-derived cells produced using our protocol undergo hypertrophy, although at a very slow rate. There were no signs of teratoma or other tumor formation at any of the transplanted sites.

Bottom Line: Defects in articular cartilage ultimately result in loss of joint function.The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation.The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.

View Article: PubMed Central - PubMed

Affiliation: Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.

Show MeSH
Related in: MedlinePlus