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Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer.

Zhang YT, Zhang YL, Chen SX, Yin GH, Yang ZZ, Lee S, Liu CG, Zhao DD, Ma YK, Song FQ, Bennett JW, Yang FS - BMC Genomics (2015)

Bottom Line: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression.Data are available via ProteomeXchange with identifier PXD001793.We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. zhangytong_2006@163.com.

ABSTRACT

Background: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D gel, we identified 62 MeJA-responsive proteins and measured protein expression level changes.

Results: Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. Data are available via ProteomeXchange with identifier PXD001793. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). The expression levels of four important genes were determined by qRT-PCR analysis. The expression levels of these proteins did not correlate well with their translation levels. To test the defense functions of the differentially expressed proteins, expression vectors of four protein coding genes were constructed to express in-fusion proteins in E. coli. The expressed proteins were used to feed Ostrinia furnacalis, the Asian corn borer (ACB). Our results demonstrated that the recombinant proteins of pathogenesis-related protein 1 (PR1) and thioredoxin M-type, chloroplastic precursor (TRXM) showed the significant inhibition on the development of larvae and pupae.

Conclusions: We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB.

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Related in: MedlinePlus

Quantitative RT-PCR analysis of four defense genes was performed after MeJA treatment. The chart shows the expression abundance of the selected genes’ transcripts in response to MeJA treatment, * and ** indicate that values are significantly different at P < 0.05 and P < 0.01 level, respectively.
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Fig6: Quantitative RT-PCR analysis of four defense genes was performed after MeJA treatment. The chart shows the expression abundance of the selected genes’ transcripts in response to MeJA treatment, * and ** indicate that values are significantly different at P < 0.05 and P < 0.01 level, respectively.

Mentions: Four genes closely associated with plant defense were selected for qRT-PCR analysis: beta-D-glucosidase precursor gene (bgl); thioredoxin M-type, chloroplastic precursor gene (TRXM); pathogenesis-related protein 1 gene (PR1); and glycine-rich RNA-binding, abscisic acid-inducible protein gene (RAB15). The primers of these four genes and the reference gene (actin) are listed in Table 4. The qRT-PCR results are shown in Figure 6. The primers were designed using Primer Premier 5.0. The suitable restriction enzymes were added when we designed the primers for in-fusion defensive proteins. Following MeJA treatment, there was more than 1.5 fold increase in the expression levels of these four genes. The bgl (GenBank No. HQ834242), PR1 (GenBank No. HQ834244), and RAB15 (GenBank No. HQ834245) genes were up-regulated while TRXM (GenBank No. HQ834243) was down-regulated after MeJA induction.Table 4


Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer.

Zhang YT, Zhang YL, Chen SX, Yin GH, Yang ZZ, Lee S, Liu CG, Zhao DD, Ma YK, Song FQ, Bennett JW, Yang FS - BMC Genomics (2015)

Quantitative RT-PCR analysis of four defense genes was performed after MeJA treatment. The chart shows the expression abundance of the selected genes’ transcripts in response to MeJA treatment, * and ** indicate that values are significantly different at P < 0.05 and P < 0.01 level, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4375847&req=5

Fig6: Quantitative RT-PCR analysis of four defense genes was performed after MeJA treatment. The chart shows the expression abundance of the selected genes’ transcripts in response to MeJA treatment, * and ** indicate that values are significantly different at P < 0.05 and P < 0.01 level, respectively.
Mentions: Four genes closely associated with plant defense were selected for qRT-PCR analysis: beta-D-glucosidase precursor gene (bgl); thioredoxin M-type, chloroplastic precursor gene (TRXM); pathogenesis-related protein 1 gene (PR1); and glycine-rich RNA-binding, abscisic acid-inducible protein gene (RAB15). The primers of these four genes and the reference gene (actin) are listed in Table 4. The qRT-PCR results are shown in Figure 6. The primers were designed using Primer Premier 5.0. The suitable restriction enzymes were added when we designed the primers for in-fusion defensive proteins. Following MeJA treatment, there was more than 1.5 fold increase in the expression levels of these four genes. The bgl (GenBank No. HQ834242), PR1 (GenBank No. HQ834244), and RAB15 (GenBank No. HQ834245) genes were up-regulated while TRXM (GenBank No. HQ834243) was down-regulated after MeJA induction.Table 4

Bottom Line: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression.Data are available via ProteomeXchange with identifier PXD001793.We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. zhangytong_2006@163.com.

ABSTRACT

Background: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D gel, we identified 62 MeJA-responsive proteins and measured protein expression level changes.

Results: Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. Data are available via ProteomeXchange with identifier PXD001793. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). The expression levels of four important genes were determined by qRT-PCR analysis. The expression levels of these proteins did not correlate well with their translation levels. To test the defense functions of the differentially expressed proteins, expression vectors of four protein coding genes were constructed to express in-fusion proteins in E. coli. The expressed proteins were used to feed Ostrinia furnacalis, the Asian corn borer (ACB). Our results demonstrated that the recombinant proteins of pathogenesis-related protein 1 (PR1) and thioredoxin M-type, chloroplastic precursor (TRXM) showed the significant inhibition on the development of larvae and pupae.

Conclusions: We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB.

Show MeSH
Related in: MedlinePlus