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Protective effect of rutin on impairment of cognitive functions of due to antiepileptic drugs on zebrafish model.

Dubey S, Ganeshpurkar A, Bansal D, Dubey N - Indian J Pharmacol (2015 Jan-Feb)

Bottom Line: Treatment with AEDs resulted in restricted movement of zebrafish to the dark zone.Similarly, time spent in the light compartment by zebrafish treated with rutin was significantly (P < 0.01) higher as compared to zebrafish treated with AEDs.The results suggest a protective role of rutin on cognition impaired by AEDs.

View Article: PubMed Central - PubMed

Affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.

ABSTRACT

Aim: The severity of adverse reactions due to antiepileptics is observed during initiation and early treatment in which impairment of cognitive effects are common. Since long time, herbal medicine is a natural remedy to treat neural symptoms. Phytochemicals have been proven to be potent neuro-protective agents. Rutin, a bioflavonoid is established to be nootropic in many studies. In this study, we aimed to determine the protective effect of rutin in zebrafish against the side effects produced by AEDs.

Materials and methods: Seizures were induced in zebrafish by phenylenetetrazole. Rutin pretreatment (50 mg/kg, single injection, i.p.) was done before commencement of the study. Behavioral studies were performed as: latency to move high in the tank, locomotion effects, color effect, shoal cohesion, light/dark test on Zebrafish.

Results: Treatment with rutin reverted the locomotor behavior to normal. Treatment with AEDs caused fishes to move in all regions while, in case of treatment with rutin, the response reverted to normal. Treatment with AEDs altered swimming behavior of zebrafish, however, rutin showed a positive effect over this behavior. Treatment with AEDs resulted in restricted movement of zebrafish to the dark zone. Treatment with rutin caused increased latency of zebrafish to move in the light compartment. Similarly, time spent in the light compartment by zebrafish treated with rutin was significantly (P < 0.01) higher as compared to zebrafish treated with AEDs.

Conclusion: The results suggest a protective role of rutin on cognition impaired by AEDs.

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Effect of rutin on shoal cohesion in zebrafish
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Figure 5: Effect of rutin on shoal cohesion in zebrafish

Mentions: Shoal cohesion is the phenomena in which fish prefer to swim in groups, and their group aggregation is termed as Shoal Cohesion. Normally it is seen that zebrafish swim in groups. When they were treated with AEDs, a vast difference was seen in their swimming behavior. This result shows that the effects of rutin were positive over the disrupted swimming behavior produced by the administration of AEDs [Figure 5].


Protective effect of rutin on impairment of cognitive functions of due to antiepileptic drugs on zebrafish model.

Dubey S, Ganeshpurkar A, Bansal D, Dubey N - Indian J Pharmacol (2015 Jan-Feb)

Effect of rutin on shoal cohesion in zebrafish
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375825&req=5

Figure 5: Effect of rutin on shoal cohesion in zebrafish
Mentions: Shoal cohesion is the phenomena in which fish prefer to swim in groups, and their group aggregation is termed as Shoal Cohesion. Normally it is seen that zebrafish swim in groups. When they were treated with AEDs, a vast difference was seen in their swimming behavior. This result shows that the effects of rutin were positive over the disrupted swimming behavior produced by the administration of AEDs [Figure 5].

Bottom Line: Treatment with AEDs resulted in restricted movement of zebrafish to the dark zone.Similarly, time spent in the light compartment by zebrafish treated with rutin was significantly (P < 0.01) higher as compared to zebrafish treated with AEDs.The results suggest a protective role of rutin on cognition impaired by AEDs.

View Article: PubMed Central - PubMed

Affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.

ABSTRACT

Aim: The severity of adverse reactions due to antiepileptics is observed during initiation and early treatment in which impairment of cognitive effects are common. Since long time, herbal medicine is a natural remedy to treat neural symptoms. Phytochemicals have been proven to be potent neuro-protective agents. Rutin, a bioflavonoid is established to be nootropic in many studies. In this study, we aimed to determine the protective effect of rutin in zebrafish against the side effects produced by AEDs.

Materials and methods: Seizures were induced in zebrafish by phenylenetetrazole. Rutin pretreatment (50 mg/kg, single injection, i.p.) was done before commencement of the study. Behavioral studies were performed as: latency to move high in the tank, locomotion effects, color effect, shoal cohesion, light/dark test on Zebrafish.

Results: Treatment with rutin reverted the locomotor behavior to normal. Treatment with AEDs caused fishes to move in all regions while, in case of treatment with rutin, the response reverted to normal. Treatment with AEDs altered swimming behavior of zebrafish, however, rutin showed a positive effect over this behavior. Treatment with AEDs resulted in restricted movement of zebrafish to the dark zone. Treatment with rutin caused increased latency of zebrafish to move in the light compartment. Similarly, time spent in the light compartment by zebrafish treated with rutin was significantly (P < 0.01) higher as compared to zebrafish treated with AEDs.

Conclusion: The results suggest a protective role of rutin on cognition impaired by AEDs.

Show MeSH
Related in: MedlinePlus