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Protective effect of simvastatin and rosuvastatin on trinitrobenzene sulfonic acid-induced colitis in rats.

Maheshwari RA, Balaraman R, Sailor GU, Sen DB - Indian J Pharmacol (2015 Jan-Feb)

Bottom Line: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity.Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis.These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Vadodara, Gujarat, India.

ABSTRACT

Objective: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity. This study was carried out to evaluate the effect of simvastatin or rosuvastatin on the extent of colonic mucosal damage and on the inflammatory response in trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis.

Materials and methods: Ulcerative colitis was induced by single intrarectal injection of 120 mg/kg TNBS. Test groups were treated with simvastatin (10 mg/kg, p.o.) or rosuvastatin (10 mg/kg, p.o.). Colonic mucosal inflammation was evaluated clinically, biochemically, and histologically.

Result: Disease activity index score in TNBS-treated rats, as determined by weight loss, stool consistency, fecal occult blood, were significantly lowers in simvastatin or rosuvastatin-treated rats than TNBS-treated animals. Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis. Simvastatin and rosuvastatin also inhibited the increase in oxidative stress levels after TNBS administration.

Conclusions: These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

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Related in: MedlinePlus

Effect of simvastatin or rosuvastatin on colon length and weight. Values are expressed as mean±SEM, n=6. ###P<0.001 as compared to control group, *P<0.05, **P<0.01, ***P<0.001 as compared to TNBS control group
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Figure 3: Effect of simvastatin or rosuvastatin on colon length and weight. Values are expressed as mean±SEM, n=6. ###P<0.001 as compared to control group, *P<0.05, **P<0.01, ***P<0.001 as compared to TNBS control group

Mentions: Intrarectal administration of TNBS produced severe inflammation in rat colon, with hemorrhagic spots and extensive disruption [Figure 2]. There was a significant (P < 0.001) decrease of colon length 7 days after TNBS administration as compared to control the group. In contrast, decreased in colon length after TNBS administration was significantly reversed by simvastatin or rosuvastatin [Figure 3a]. The colon weight was significantly (P < 0.001) increased after TNBS administration as compared to control group, while in treatment with simvastatin or rosuvastatin significantly (P < 0.05, P < 0.01) decreased in colon weight, respectively, as compared to TNBS alone treated rats [Figure 3b].


Protective effect of simvastatin and rosuvastatin on trinitrobenzene sulfonic acid-induced colitis in rats.

Maheshwari RA, Balaraman R, Sailor GU, Sen DB - Indian J Pharmacol (2015 Jan-Feb)

Effect of simvastatin or rosuvastatin on colon length and weight. Values are expressed as mean±SEM, n=6. ###P<0.001 as compared to control group, *P<0.05, **P<0.01, ***P<0.001 as compared to TNBS control group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375812&req=5

Figure 3: Effect of simvastatin or rosuvastatin on colon length and weight. Values are expressed as mean±SEM, n=6. ###P<0.001 as compared to control group, *P<0.05, **P<0.01, ***P<0.001 as compared to TNBS control group
Mentions: Intrarectal administration of TNBS produced severe inflammation in rat colon, with hemorrhagic spots and extensive disruption [Figure 2]. There was a significant (P < 0.001) decrease of colon length 7 days after TNBS administration as compared to control the group. In contrast, decreased in colon length after TNBS administration was significantly reversed by simvastatin or rosuvastatin [Figure 3a]. The colon weight was significantly (P < 0.001) increased after TNBS administration as compared to control group, while in treatment with simvastatin or rosuvastatin significantly (P < 0.05, P < 0.01) decreased in colon weight, respectively, as compared to TNBS alone treated rats [Figure 3b].

Bottom Line: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity.Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis.These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Vadodara, Gujarat, India.

ABSTRACT

Objective: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity. This study was carried out to evaluate the effect of simvastatin or rosuvastatin on the extent of colonic mucosal damage and on the inflammatory response in trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis.

Materials and methods: Ulcerative colitis was induced by single intrarectal injection of 120 mg/kg TNBS. Test groups were treated with simvastatin (10 mg/kg, p.o.) or rosuvastatin (10 mg/kg, p.o.). Colonic mucosal inflammation was evaluated clinically, biochemically, and histologically.

Result: Disease activity index score in TNBS-treated rats, as determined by weight loss, stool consistency, fecal occult blood, were significantly lowers in simvastatin or rosuvastatin-treated rats than TNBS-treated animals. Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis. Simvastatin and rosuvastatin also inhibited the increase in oxidative stress levels after TNBS administration.

Conclusions: These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

Show MeSH
Related in: MedlinePlus