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Protective effect of simvastatin and rosuvastatin on trinitrobenzene sulfonic acid-induced colitis in rats.

Maheshwari RA, Balaraman R, Sailor GU, Sen DB - Indian J Pharmacol (2015 Jan-Feb)

Bottom Line: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity.Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis.These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Vadodara, Gujarat, India.

ABSTRACT

Objective: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity. This study was carried out to evaluate the effect of simvastatin or rosuvastatin on the extent of colonic mucosal damage and on the inflammatory response in trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis.

Materials and methods: Ulcerative colitis was induced by single intrarectal injection of 120 mg/kg TNBS. Test groups were treated with simvastatin (10 mg/kg, p.o.) or rosuvastatin (10 mg/kg, p.o.). Colonic mucosal inflammation was evaluated clinically, biochemically, and histologically.

Result: Disease activity index score in TNBS-treated rats, as determined by weight loss, stool consistency, fecal occult blood, were significantly lowers in simvastatin or rosuvastatin-treated rats than TNBS-treated animals. Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis. Simvastatin and rosuvastatin also inhibited the increase in oxidative stress levels after TNBS administration.

Conclusions: These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

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Effect of simvastatin or rosuvastatin on disease activity index score of trinitrobenzene sulfonic acid -induced colitis in rats
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Figure 1: Effect of simvastatin or rosuvastatin on disease activity index score of trinitrobenzene sulfonic acid -induced colitis in rats

Mentions: We did not observe any mortality among the experimental groups. However, single dose administration of TNBS in colitis control group caused acute colitis and diarrhea followed by rectal bleeding and severe weight loss from day 6 to day 12. DAI score, an indicator of the severity of intestinal inflammation, was a significant increase in the colitis control group. In the simvastatin or rosuvastatin-treated groups, we did not observe bloody stools, stools were better formed, and weight loss was lessened. DAI score was significantly decreased in the rats treated with simvastatin or rosuvastatin than TNBS alone treated rats [Figure 1].


Protective effect of simvastatin and rosuvastatin on trinitrobenzene sulfonic acid-induced colitis in rats.

Maheshwari RA, Balaraman R, Sailor GU, Sen DB - Indian J Pharmacol (2015 Jan-Feb)

Effect of simvastatin or rosuvastatin on disease activity index score of trinitrobenzene sulfonic acid -induced colitis in rats
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375812&req=5

Figure 1: Effect of simvastatin or rosuvastatin on disease activity index score of trinitrobenzene sulfonic acid -induced colitis in rats
Mentions: We did not observe any mortality among the experimental groups. However, single dose administration of TNBS in colitis control group caused acute colitis and diarrhea followed by rectal bleeding and severe weight loss from day 6 to day 12. DAI score, an indicator of the severity of intestinal inflammation, was a significant increase in the colitis control group. In the simvastatin or rosuvastatin-treated groups, we did not observe bloody stools, stools were better formed, and weight loss was lessened. DAI score was significantly decreased in the rats treated with simvastatin or rosuvastatin than TNBS alone treated rats [Figure 1].

Bottom Line: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity.Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis.These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Vadodara, Gujarat, India.

ABSTRACT

Objective: Statins have anti-inflammatory effects that are not directly related to their cholesterol lowering activity. This study was carried out to evaluate the effect of simvastatin or rosuvastatin on the extent of colonic mucosal damage and on the inflammatory response in trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis.

Materials and methods: Ulcerative colitis was induced by single intrarectal injection of 120 mg/kg TNBS. Test groups were treated with simvastatin (10 mg/kg, p.o.) or rosuvastatin (10 mg/kg, p.o.). Colonic mucosal inflammation was evaluated clinically, biochemically, and histologically.

Result: Disease activity index score in TNBS-treated rats, as determined by weight loss, stool consistency, fecal occult blood, were significantly lowers in simvastatin or rosuvastatin-treated rats than TNBS-treated animals. Simvastatin or rosuvastatin counteracted the reduction in colon length, decreased colon weight, neutrophil accumulation, and tumor necrosis factor-alpha level in TNBS-induced colitis. Simvastatin and rosuvastatin also inhibited the increase in oxidative stress levels after TNBS administration.

Conclusions: These results suggest that simvastatin and rosuvastatin significantly ameliorate experimental colitis in rats, and these effects could be explained by their anti-inflammatory and antioxidant activity.

Show MeSH
Related in: MedlinePlus