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Aminoglycosides induce fragility of human red cell membrane: an in vitro study.

Alnakshbandi AA - Indian J Pharmacol (2015 Jan-Feb)

Bottom Line: This study aimed to find the effect of aminoglycosides on the human red cell membrane using osmotic fragility test.The effects of aminoglycosides were evaluated by osmotic fragility test using fresh human blood in the presence of aminoglycosides in concentrations of 10-160 μg/mL.The hemolysis did not depend on the concentration of aminoglycosides.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Hawler Medical College, Erbil, Iraq.

ABSTRACT

Objectives: It is well-known that aminoglycosides are ototoxic and nephrotoxic. Recent advances in pharmacology research suggest that the red cell used as a carrier of aminoglycosides. This study aimed to find the effect of aminoglycosides on the human red cell membrane using osmotic fragility test.

Materials and methods: This study was conducted in Rizgari Teaching Hospital in Erbil, Iraq. The effect of aminoglycosides, namely gentamicin, amikacin, and spectinomycin, on human red cells was investigated. The effects of aminoglycosides were evaluated by osmotic fragility test using fresh human blood in the presence of aminoglycosides in concentrations of 10-160 μg/mL.

Results: The results showed that aminoglycosides drugs shifted the osmotic fragility curve to some extent, and this effect was well observed with spectinomycin. The hemolysis did not depend on the concentration of aminoglycosides. The concentration of sodium chloride to induced 50% hemolysis is higher in presence of gentamicin, amikacin and spectinomycin (at 160 μg/mL) than corresponding control and this account to an increment in hemolysis percents of 1.88, 1.5 and 1.06%, respectively.

Conclusion: Aminoglycosides induce human red cell membrane fragility in a concentration-independent manner.

Show MeSH
Effects of gentamicin (a), amikacin (b) and spectinomycin (c) on the osmotic fragility of red cells and the cut-off point of sodium chloride that does not induce hemolysis (d)
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Figure 1: Effects of gentamicin (a), amikacin (b) and spectinomycin (c) on the osmotic fragility of red cells and the cut-off point of sodium chloride that does not induce hemolysis (d)

Mentions: Figure 1 shows that gentamicin at 160 μg/mL shifts the osmotic fragility test slightly to the left while at low concentration (at 20 μg/mL) shifts slightly the curve toward the right side. Amikacin shifts the curve of osmotic fragility toward the right side (i.e. hemolysis of red cells at high NaCl concentration compared with control) at 160 μg/mL [Figure 1]. The effect of spectinomycin on the human red cell fragility is more obvious than gentamycin and amikacin. It shifts the osmotic fragility curve toward the right side at higher tested concentrations [Figure 1].


Aminoglycosides induce fragility of human red cell membrane: an in vitro study.

Alnakshbandi AA - Indian J Pharmacol (2015 Jan-Feb)

Effects of gentamicin (a), amikacin (b) and spectinomycin (c) on the osmotic fragility of red cells and the cut-off point of sodium chloride that does not induce hemolysis (d)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375805&req=5

Figure 1: Effects of gentamicin (a), amikacin (b) and spectinomycin (c) on the osmotic fragility of red cells and the cut-off point of sodium chloride that does not induce hemolysis (d)
Mentions: Figure 1 shows that gentamicin at 160 μg/mL shifts the osmotic fragility test slightly to the left while at low concentration (at 20 μg/mL) shifts slightly the curve toward the right side. Amikacin shifts the curve of osmotic fragility toward the right side (i.e. hemolysis of red cells at high NaCl concentration compared with control) at 160 μg/mL [Figure 1]. The effect of spectinomycin on the human red cell fragility is more obvious than gentamycin and amikacin. It shifts the osmotic fragility curve toward the right side at higher tested concentrations [Figure 1].

Bottom Line: This study aimed to find the effect of aminoglycosides on the human red cell membrane using osmotic fragility test.The effects of aminoglycosides were evaluated by osmotic fragility test using fresh human blood in the presence of aminoglycosides in concentrations of 10-160 μg/mL.The hemolysis did not depend on the concentration of aminoglycosides.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Hawler Medical College, Erbil, Iraq.

ABSTRACT

Objectives: It is well-known that aminoglycosides are ototoxic and nephrotoxic. Recent advances in pharmacology research suggest that the red cell used as a carrier of aminoglycosides. This study aimed to find the effect of aminoglycosides on the human red cell membrane using osmotic fragility test.

Materials and methods: This study was conducted in Rizgari Teaching Hospital in Erbil, Iraq. The effect of aminoglycosides, namely gentamicin, amikacin, and spectinomycin, on human red cells was investigated. The effects of aminoglycosides were evaluated by osmotic fragility test using fresh human blood in the presence of aminoglycosides in concentrations of 10-160 μg/mL.

Results: The results showed that aminoglycosides drugs shifted the osmotic fragility curve to some extent, and this effect was well observed with spectinomycin. The hemolysis did not depend on the concentration of aminoglycosides. The concentration of sodium chloride to induced 50% hemolysis is higher in presence of gentamicin, amikacin and spectinomycin (at 160 μg/mL) than corresponding control and this account to an increment in hemolysis percents of 1.88, 1.5 and 1.06%, respectively.

Conclusion: Aminoglycosides induce human red cell membrane fragility in a concentration-independent manner.

Show MeSH