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Interaction of aqueous leaf extract of Aegle marmelos (L.) Corr. with cholinergic, serotonergic and adrenergic receptors: an ex vivo study.

Kumar S, Mahaseth RK, Tiwari M, Sehgal R, Rajora P, Mathur R - Indian J Pharmacol (2015 Jan-Feb)

Bottom Line: Cumulative concentration-response curves (CRC) were constructed at various doses on each tissue for AM and respective standard agonist.The relaxant effect, produced by AM (0.2 mg/ml) on carbachol (10(-5) M) precontracted rat tracheal chain, was also inhibited significantly (P < 0.05) by propranolol (1 ng/ml).It may be concluded that AM possesses agonistic activity on cholinergic, serotonergic and adrenergic receptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.

ABSTRACT

Objectives: The aim was to study interaction of aqueous leaf extract of Aegle marmelos (AM) with cholinergic, serotonergic, and adrenergic receptor systems using appropriate rat tissues-ileum, fundus and tracheal chain, respectively.

Materials and methods: Cumulative concentration-response curves (CRC) were constructed at various doses on each tissue for AM and respective standard agonist. The CRC was again plotted in presence and absence of respective standard antagonist to confirm the interaction of receptor system and AM.

Results: AM induced concentration-dependent contractions in isolated rat ileum (0.2-6.4 mg/ml) and fundus (0.2-3.2 mg/ml) that were inhibited significantly (P < 0.05) in the presence of atropine (10(-7) M) and ketanserin (10(-6) M), respectively. The relaxant effect, produced by AM (0.2 mg/ml) on carbachol (10(-5) M) precontracted rat tracheal chain, was also inhibited significantly (P < 0.05) by propranolol (1 ng/ml).

Conclusion: It may be concluded that AM possesses agonistic activity on cholinergic, serotonergic and adrenergic receptors.

Show MeSH

Related in: MedlinePlus

Interaction of Aegle marmelos (AM) with serotonergic receptors. Cumulative concentration-response curves of (a) 5-HT alone and 5-HT in the presence of ketanserin (10−6 M) (b) AM alone and AM in the presence of ketanserin (10−6 M) (c) 5-HT and AM on isolated rat fundus. Values are expressed as mean ± standard error of the mean (n = 3)
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Figure 2: Interaction of Aegle marmelos (AM) with serotonergic receptors. Cumulative concentration-response curves of (a) 5-HT alone and 5-HT in the presence of ketanserin (10−6 M) (b) AM alone and AM in the presence of ketanserin (10−6 M) (c) 5-HT and AM on isolated rat fundus. Values are expressed as mean ± standard error of the mean (n = 3)

Mentions: Concentration-dependent contractions were induced by 5-HT as well as AM in isolated rat fundus and both showed a right ward shift in CRC in the presence of ketanserin (10−6 M) [Figure 2a and b]. The EC50 of 5-HT after ketanserin (3.6 ± 0.15 × 10−8 M) was significantly higher (P < 0.05) than EC50 of 5-HT alone (1.0 ± 0.37 × 10−8 M). The EC50 of AM after ketanserin (10−6 M) (0.62 ± 0.03 mg/ml) was significantly higher (P < 0.05) than EC50 of AM alone (0.28 ± 0.03 mg/ml). The CRC of AM was shifted to the right side of 5-HT [Figure 2c] and the EC50 of AM (0.28 ± 0.03 mg/ml) was significantly higher (P < 0.05) than that of 5-HT (4.1 ± 1.5 × 10−6 mg/ ml). The maximal response of AM (827.44 ± 69.24 mg) was found significantly lesser (P < 0.05) than that of 5-HT (1110.93 ± 35.83 mg) [Figure 2c].


Interaction of aqueous leaf extract of Aegle marmelos (L.) Corr. with cholinergic, serotonergic and adrenergic receptors: an ex vivo study.

Kumar S, Mahaseth RK, Tiwari M, Sehgal R, Rajora P, Mathur R - Indian J Pharmacol (2015 Jan-Feb)

Interaction of Aegle marmelos (AM) with serotonergic receptors. Cumulative concentration-response curves of (a) 5-HT alone and 5-HT in the presence of ketanserin (10−6 M) (b) AM alone and AM in the presence of ketanserin (10−6 M) (c) 5-HT and AM on isolated rat fundus. Values are expressed as mean ± standard error of the mean (n = 3)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4375803&req=5

Figure 2: Interaction of Aegle marmelos (AM) with serotonergic receptors. Cumulative concentration-response curves of (a) 5-HT alone and 5-HT in the presence of ketanserin (10−6 M) (b) AM alone and AM in the presence of ketanserin (10−6 M) (c) 5-HT and AM on isolated rat fundus. Values are expressed as mean ± standard error of the mean (n = 3)
Mentions: Concentration-dependent contractions were induced by 5-HT as well as AM in isolated rat fundus and both showed a right ward shift in CRC in the presence of ketanserin (10−6 M) [Figure 2a and b]. The EC50 of 5-HT after ketanserin (3.6 ± 0.15 × 10−8 M) was significantly higher (P < 0.05) than EC50 of 5-HT alone (1.0 ± 0.37 × 10−8 M). The EC50 of AM after ketanserin (10−6 M) (0.62 ± 0.03 mg/ml) was significantly higher (P < 0.05) than EC50 of AM alone (0.28 ± 0.03 mg/ml). The CRC of AM was shifted to the right side of 5-HT [Figure 2c] and the EC50 of AM (0.28 ± 0.03 mg/ml) was significantly higher (P < 0.05) than that of 5-HT (4.1 ± 1.5 × 10−6 mg/ ml). The maximal response of AM (827.44 ± 69.24 mg) was found significantly lesser (P < 0.05) than that of 5-HT (1110.93 ± 35.83 mg) [Figure 2c].

Bottom Line: Cumulative concentration-response curves (CRC) were constructed at various doses on each tissue for AM and respective standard agonist.The relaxant effect, produced by AM (0.2 mg/ml) on carbachol (10(-5) M) precontracted rat tracheal chain, was also inhibited significantly (P < 0.05) by propranolol (1 ng/ml).It may be concluded that AM possesses agonistic activity on cholinergic, serotonergic and adrenergic receptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.

ABSTRACT

Objectives: The aim was to study interaction of aqueous leaf extract of Aegle marmelos (AM) with cholinergic, serotonergic, and adrenergic receptor systems using appropriate rat tissues-ileum, fundus and tracheal chain, respectively.

Materials and methods: Cumulative concentration-response curves (CRC) were constructed at various doses on each tissue for AM and respective standard agonist. The CRC was again plotted in presence and absence of respective standard antagonist to confirm the interaction of receptor system and AM.

Results: AM induced concentration-dependent contractions in isolated rat ileum (0.2-6.4 mg/ml) and fundus (0.2-3.2 mg/ml) that were inhibited significantly (P < 0.05) in the presence of atropine (10(-7) M) and ketanserin (10(-6) M), respectively. The relaxant effect, produced by AM (0.2 mg/ml) on carbachol (10(-5) M) precontracted rat tracheal chain, was also inhibited significantly (P < 0.05) by propranolol (1 ng/ml).

Conclusion: It may be concluded that AM possesses agonistic activity on cholinergic, serotonergic and adrenergic receptors.

Show MeSH
Related in: MedlinePlus