Limits...
Spatial variability of microbial assemblages associated with a dominant habitat-forming seaweed.

Campbell AH, Marzinelli EM, Gelber J, Steinberg PD - Front Microbiol (2015)

Bottom Line: We used the disappearance of the dominant canopy-forming fucoid Phyllospora comosa from the metropolitan coast of Sydney, NSW, Australia as a model system to study these interactions.We transplanted Phyllospora individuals from nearby, extant populations back onto reefs in Sydney to test whether bacterial assemblages associated with seaweed surfaces would be influenced by (i) the host itself, independently of where it occurs, (ii) the type of habitat where the host occurs, or (iii) site-specific differences.Rather, they were primarily influenced by local, site-specific conditions with some evidence for host-specificity in some cases.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Bio-Innovation and School of Biological, Earth and Environmental Sciences, University of New South Wales Sydney, NSW, Australia ; Sydney Institute of Marine Science, Mosman NSW, Australia.

ABSTRACT
Macroalgal surfaces support abundant and diverse microorganisms within biofilms, which are often involved in fundamental functions relating to the health and defense of their seaweed hosts, including algal development, facilitation of spore release, and chemical antifouling. Given these intimate and important interactions, environmental changes have the potential to negatively impact macroalgae by disrupting seaweed-microbe interactions. We used the disappearance of the dominant canopy-forming fucoid Phyllospora comosa from the metropolitan coast of Sydney, NSW, Australia as a model system to study these interactions. We transplanted Phyllospora individuals from nearby, extant populations back onto reefs in Sydney to test whether bacterial assemblages associated with seaweed surfaces would be influenced by (i) the host itself, independently of where it occurs, (ii) the type of habitat where the host occurs, or (iii) site-specific differences. Analyses of bacterial DNA fingerprints (terminal fragment length polymorphisms) indicated that assemblages of bacteria on Phyllospora were not habitat-specific. Rather, they were primarily influenced by local, site-specific conditions with some evidence for host-specificity in some cases. This could suggest a lottery model of host-surface colonization, by which hosts are colonized by 'suitable' bacteria available in the local species pool, resulting in high variability in assemblage structure across sites, but where some species in the community are specific to the host and possibly influenced by differences in host traits.

No MeSH data available.


Related in: MedlinePlus

nMDS based on Bray-Curtis measure of square-root transformed relative abundances (structure) or Jaccard measure (composition) of bacterial TRFLP on Phyllospora comosa originally from Croa (empty symbols) or Palm Beach (filled symbols) in the first experiment. Treatments: Undisturbed (green symbols), Disturbed (black symbols), Translocated (blue symbols), Transplanted (red symbols). Destination places: Croa (circles), Palm Beach (squares), Long Bay (downward triangle), Cape Banks (upward triangle).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4374473&req=5

Figure 1: nMDS based on Bray-Curtis measure of square-root transformed relative abundances (structure) or Jaccard measure (composition) of bacterial TRFLP on Phyllospora comosa originally from Croa (empty symbols) or Palm Beach (filled symbols) in the first experiment. Treatments: Undisturbed (green symbols), Disturbed (black symbols), Translocated (blue symbols), Transplanted (red symbols). Destination places: Croa (circles), Palm Beach (squares), Long Bay (downward triangle), Cape Banks (upward triangle).

Mentions: We detected a total of 795 microbial TRFs in this study. TRF sizes were searched against the RDP and SILVA databases using MICA (Shyu et al., 2007). No fragment was found to correspond with the predicted size of chloroplast or plastid 16S rRNA genes contained within the two databases. This indicates that the TRFLP profiles are not contaminated by host DNA or epiphytic algae and thus contain only bacterial or archaeal 16s rRNA gene information. In the first experiment, the structure and composition of bacterial fingerprints differed among treatments, but these differed according to the transplants place of origin (i.e., there was Treatment × Place interaction, Table 1A). Bacteria on algae transplanted from Palm Beach to Sydney metro differed from those on individuals that remained in Palm Beach undisturbed, on disturbed individuals returned to Palm Beach and on those individuals translocated to Croa. Although bacteria on translocated algae differed from those on undisturbed algae, there were no differences between the translocated and disturbed treatments (Table 1A). Algae originally from Croa had different bacterial TRFLP across all treatments (Table 1A; Figure 1).


Spatial variability of microbial assemblages associated with a dominant habitat-forming seaweed.

Campbell AH, Marzinelli EM, Gelber J, Steinberg PD - Front Microbiol (2015)

nMDS based on Bray-Curtis measure of square-root transformed relative abundances (structure) or Jaccard measure (composition) of bacterial TRFLP on Phyllospora comosa originally from Croa (empty symbols) or Palm Beach (filled symbols) in the first experiment. Treatments: Undisturbed (green symbols), Disturbed (black symbols), Translocated (blue symbols), Transplanted (red symbols). Destination places: Croa (circles), Palm Beach (squares), Long Bay (downward triangle), Cape Banks (upward triangle).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374473&req=5

Figure 1: nMDS based on Bray-Curtis measure of square-root transformed relative abundances (structure) or Jaccard measure (composition) of bacterial TRFLP on Phyllospora comosa originally from Croa (empty symbols) or Palm Beach (filled symbols) in the first experiment. Treatments: Undisturbed (green symbols), Disturbed (black symbols), Translocated (blue symbols), Transplanted (red symbols). Destination places: Croa (circles), Palm Beach (squares), Long Bay (downward triangle), Cape Banks (upward triangle).
Mentions: We detected a total of 795 microbial TRFs in this study. TRF sizes were searched against the RDP and SILVA databases using MICA (Shyu et al., 2007). No fragment was found to correspond with the predicted size of chloroplast or plastid 16S rRNA genes contained within the two databases. This indicates that the TRFLP profiles are not contaminated by host DNA or epiphytic algae and thus contain only bacterial or archaeal 16s rRNA gene information. In the first experiment, the structure and composition of bacterial fingerprints differed among treatments, but these differed according to the transplants place of origin (i.e., there was Treatment × Place interaction, Table 1A). Bacteria on algae transplanted from Palm Beach to Sydney metro differed from those on individuals that remained in Palm Beach undisturbed, on disturbed individuals returned to Palm Beach and on those individuals translocated to Croa. Although bacteria on translocated algae differed from those on undisturbed algae, there were no differences between the translocated and disturbed treatments (Table 1A). Algae originally from Croa had different bacterial TRFLP across all treatments (Table 1A; Figure 1).

Bottom Line: We used the disappearance of the dominant canopy-forming fucoid Phyllospora comosa from the metropolitan coast of Sydney, NSW, Australia as a model system to study these interactions.We transplanted Phyllospora individuals from nearby, extant populations back onto reefs in Sydney to test whether bacterial assemblages associated with seaweed surfaces would be influenced by (i) the host itself, independently of where it occurs, (ii) the type of habitat where the host occurs, or (iii) site-specific differences.Rather, they were primarily influenced by local, site-specific conditions with some evidence for host-specificity in some cases.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Bio-Innovation and School of Biological, Earth and Environmental Sciences, University of New South Wales Sydney, NSW, Australia ; Sydney Institute of Marine Science, Mosman NSW, Australia.

ABSTRACT
Macroalgal surfaces support abundant and diverse microorganisms within biofilms, which are often involved in fundamental functions relating to the health and defense of their seaweed hosts, including algal development, facilitation of spore release, and chemical antifouling. Given these intimate and important interactions, environmental changes have the potential to negatively impact macroalgae by disrupting seaweed-microbe interactions. We used the disappearance of the dominant canopy-forming fucoid Phyllospora comosa from the metropolitan coast of Sydney, NSW, Australia as a model system to study these interactions. We transplanted Phyllospora individuals from nearby, extant populations back onto reefs in Sydney to test whether bacterial assemblages associated with seaweed surfaces would be influenced by (i) the host itself, independently of where it occurs, (ii) the type of habitat where the host occurs, or (iii) site-specific differences. Analyses of bacterial DNA fingerprints (terminal fragment length polymorphisms) indicated that assemblages of bacteria on Phyllospora were not habitat-specific. Rather, they were primarily influenced by local, site-specific conditions with some evidence for host-specificity in some cases. This could suggest a lottery model of host-surface colonization, by which hosts are colonized by 'suitable' bacteria available in the local species pool, resulting in high variability in assemblage structure across sites, but where some species in the community are specific to the host and possibly influenced by differences in host traits.

No MeSH data available.


Related in: MedlinePlus