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Secretory function in subplate neurons during cortical development.

Kondo S, Al-Hasani H, Hoerder-Suabedissen A, Wang WZ, Molnár Z - Front Neurosci (2015)

Bottom Line: By comparing gene expression between subplate and layer 6, we found that several genes encoding secreted proteins are highly expressed in subplate neurons.One of these secreted proteins, neuroserpin, encoded by the serpini1 gene, is localized to the ER in subplate cells.We propose that subplate might influence cortical circuit formation through a transient secretory function.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford Oxford, UK.

ABSTRACT
Subplate cells are among the first generated neurons in the mammalian cerebral cortex and have been implicated in the establishment of cortical wiring. In rodents some subplate neurons persist into adulthood. Here we would like to highlight several converging findings which suggest a novel secretory function of subplate neurons during cortical development. Throughout the postnatal period in rodents, subplate neurons have highly developed rough endoplasmic reticulum (ER) and are under an ER stress condition. By comparing gene expression between subplate and layer 6, we found that several genes encoding secreted proteins are highly expressed in subplate neurons. One of these secreted proteins, neuroserpin, encoded by the serpini1 gene, is localized to the ER in subplate cells. We propose that subplate might influence cortical circuit formation through a transient secretory function.

No MeSH data available.


Subplate neurons have large cytoplasm with large amounts of Nissl substance and are under ER stress condition at P8. Nissl staining in coronal section of P8 (A,B) and adult (E,F) mouse brain. Note, subplate neurons (and some layer 5 and 2–3 neurons) have voluminous cytoplasm with large amounts of Nissl substance (arrow and inset, B) at P8. Subplate neurons in adult mouse have relatively small cytoplasm (arrow and inset, F). Immunohistochemistry for anti-KDEL antibody, which recognizes BiP/GRP78 (Okiyoneda et al., 2004), in coronal section of P8 mouse (C,D). Layer 5, layer 2–3, some layer 6, and subplate neurons express strong BiP immunoreactivity. Immunohistochemistry for anti-KDEL in coronal section of adult mouse (G,H). Scale bars: 200 μm (A,C,E,G), 50 μm (B,D,F,H), 10 μm (inset in B and F).
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Figure 1: Subplate neurons have large cytoplasm with large amounts of Nissl substance and are under ER stress condition at P8. Nissl staining in coronal section of P8 (A,B) and adult (E,F) mouse brain. Note, subplate neurons (and some layer 5 and 2–3 neurons) have voluminous cytoplasm with large amounts of Nissl substance (arrow and inset, B) at P8. Subplate neurons in adult mouse have relatively small cytoplasm (arrow and inset, F). Immunohistochemistry for anti-KDEL antibody, which recognizes BiP/GRP78 (Okiyoneda et al., 2004), in coronal section of P8 mouse (C,D). Layer 5, layer 2–3, some layer 6, and subplate neurons express strong BiP immunoreactivity. Immunohistochemistry for anti-KDEL in coronal section of adult mouse (G,H). Scale bars: 200 μm (A,C,E,G), 50 μm (B,D,F,H), 10 μm (inset in B and F).

Mentions: To characterize the morphology and the functional state of subplate neurons in postnatal and adult rodents, we analyzed Nissl stained coronal sections of P8 mouse brains (Figures 1A,B) and adult mouse brains (Figures 1E,F). At P8, extensive Nissl substance was detected in the large pyramidal cells of layer 5. Plentiful Nissl substance was also observed in layer 2/3 and subplate neurons (Figure 1A), suggesting that these neurons produce a large amount of proteins at P8. The morphological features of subplate neurons (Figure 1B) are surprisingly similar to those of plasma cells, which also have a large, ovoid cell body with non-central distribution of nucleus and basophilic cytoplasm due to their richness in rough ER (Bloom and Fawcett, 1968).


Secretory function in subplate neurons during cortical development.

Kondo S, Al-Hasani H, Hoerder-Suabedissen A, Wang WZ, Molnár Z - Front Neurosci (2015)

Subplate neurons have large cytoplasm with large amounts of Nissl substance and are under ER stress condition at P8. Nissl staining in coronal section of P8 (A,B) and adult (E,F) mouse brain. Note, subplate neurons (and some layer 5 and 2–3 neurons) have voluminous cytoplasm with large amounts of Nissl substance (arrow and inset, B) at P8. Subplate neurons in adult mouse have relatively small cytoplasm (arrow and inset, F). Immunohistochemistry for anti-KDEL antibody, which recognizes BiP/GRP78 (Okiyoneda et al., 2004), in coronal section of P8 mouse (C,D). Layer 5, layer 2–3, some layer 6, and subplate neurons express strong BiP immunoreactivity. Immunohistochemistry for anti-KDEL in coronal section of adult mouse (G,H). Scale bars: 200 μm (A,C,E,G), 50 μm (B,D,F,H), 10 μm (inset in B and F).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 1: Subplate neurons have large cytoplasm with large amounts of Nissl substance and are under ER stress condition at P8. Nissl staining in coronal section of P8 (A,B) and adult (E,F) mouse brain. Note, subplate neurons (and some layer 5 and 2–3 neurons) have voluminous cytoplasm with large amounts of Nissl substance (arrow and inset, B) at P8. Subplate neurons in adult mouse have relatively small cytoplasm (arrow and inset, F). Immunohistochemistry for anti-KDEL antibody, which recognizes BiP/GRP78 (Okiyoneda et al., 2004), in coronal section of P8 mouse (C,D). Layer 5, layer 2–3, some layer 6, and subplate neurons express strong BiP immunoreactivity. Immunohistochemistry for anti-KDEL in coronal section of adult mouse (G,H). Scale bars: 200 μm (A,C,E,G), 50 μm (B,D,F,H), 10 μm (inset in B and F).
Mentions: To characterize the morphology and the functional state of subplate neurons in postnatal and adult rodents, we analyzed Nissl stained coronal sections of P8 mouse brains (Figures 1A,B) and adult mouse brains (Figures 1E,F). At P8, extensive Nissl substance was detected in the large pyramidal cells of layer 5. Plentiful Nissl substance was also observed in layer 2/3 and subplate neurons (Figure 1A), suggesting that these neurons produce a large amount of proteins at P8. The morphological features of subplate neurons (Figure 1B) are surprisingly similar to those of plasma cells, which also have a large, ovoid cell body with non-central distribution of nucleus and basophilic cytoplasm due to their richness in rough ER (Bloom and Fawcett, 1968).

Bottom Line: By comparing gene expression between subplate and layer 6, we found that several genes encoding secreted proteins are highly expressed in subplate neurons.One of these secreted proteins, neuroserpin, encoded by the serpini1 gene, is localized to the ER in subplate cells.We propose that subplate might influence cortical circuit formation through a transient secretory function.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford Oxford, UK.

ABSTRACT
Subplate cells are among the first generated neurons in the mammalian cerebral cortex and have been implicated in the establishment of cortical wiring. In rodents some subplate neurons persist into adulthood. Here we would like to highlight several converging findings which suggest a novel secretory function of subplate neurons during cortical development. Throughout the postnatal period in rodents, subplate neurons have highly developed rough endoplasmic reticulum (ER) and are under an ER stress condition. By comparing gene expression between subplate and layer 6, we found that several genes encoding secreted proteins are highly expressed in subplate neurons. One of these secreted proteins, neuroserpin, encoded by the serpini1 gene, is localized to the ER in subplate cells. We propose that subplate might influence cortical circuit formation through a transient secretory function.

No MeSH data available.