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Epstein-barr virus in gastro-esophageal adenocarcinomas - single center experiences in the context of current literature.

Genitsch V, Novotny A, Seiler CA, Kröll D, Walch A, Langer R - Front Oncol (2015)

Bottom Line: No association between EBV and pT, pN, or tumor grading was found, neither was there a correlation with clinical outcome.In conclusion, EBV does not seem to play a role in the carcinogenesis of EAC.Recent reports, however, have identified specific epigenetic and genetic alterations in EBV-associated GC, which might lead to a distinct treatment approach for this specific subtype of GC in the future.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University of Bern , Bern , Switzerland.

ABSTRACT
Epstein-Barr virus (EBV)-associated gastric carcinomas (GC) represent a distinct and well-recognized subtype of gastric cancer with a prevalence of around 10% of all GC. In contrast, EBV has not been reported to play a major role in esophageal adenocarcinomas (EAC) and adenocarcinomas of the gastro-esophageal junction (GEJ). We report our experiences on EBV in collections of gastro-esophageal adenocarcinomas from two surgical centers and discuss the current state of research in this field. Tumor samples from 465 primary resected gastro-esophageal adenocarcinomas (118 EAC, 73 GEJ, and 274 GC) were investigated. Presence of EBV was determined by EBV-encoded small RNAs (EBER) in situ hybridization. Results were correlated with pathologic parameters (UICC pTNM category, Her2 status, tumor grading) and survival. EBER positivity was observed in 14 cases. None of the EAC were positive for EBER. In contrast, we observed EBER positivity in 2/73 adenocarcinomas of the GEJ (2.7%) and 12/274 GC (4.4%). These were of intestinal type (seven cases) or unclassifiable (six cases), while only one case was of diffuse type according to the Lauren classification. No association between EBV and pT, pN, or tumor grading was found, neither was there a correlation with clinical outcome. None of the EBER positive cases were Her2 positive. In conclusion, EBV does not seem to play a role in the carcinogenesis of EAC. Moreover, adenocarcinomas of the GEJ show lower rates of EBV positivity compared to GC. Our data only partially correlate with previous reports from the literature. This highlights the need for further research on this distinct entity. Recent reports, however, have identified specific epigenetic and genetic alterations in EBV-associated GC, which might lead to a distinct treatment approach for this specific subtype of GC in the future.

No MeSH data available.


Related in: MedlinePlus

Examples of EBER staining patterns and morphology in gastric carcinomas. (A,B) “Lymphoepithelioma-like” morphology; EBER positive [(A) HE; (B) EBER-ISH], (C,D) “intestinal type” morphology, EBER positive [(C) HE; (D) EBER-ISH]; (E,F) “lymphoepithelioma-like” morphology; EBER negative in the tumor, but positive in accompanying lymphocytic infiltrate [(E) HE; (F) EBER-ISH]; (G,H) “intestinal type” morphology, EBER negative [(G) HE; (H) EBER-ISH] (EBER, EBV-encoded small RNAs; ISH, in situ hybridization).
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Figure 1: Examples of EBER staining patterns and morphology in gastric carcinomas. (A,B) “Lymphoepithelioma-like” morphology; EBER positive [(A) HE; (B) EBER-ISH], (C,D) “intestinal type” morphology, EBER positive [(C) HE; (D) EBER-ISH]; (E,F) “lymphoepithelioma-like” morphology; EBER negative in the tumor, but positive in accompanying lymphocytic infiltrate [(E) HE; (F) EBER-ISH]; (G,H) “intestinal type” morphology, EBER negative [(G) HE; (H) EBER-ISH] (EBER, EBV-encoded small RNAs; ISH, in situ hybridization).

Mentions: EBV-encoded small RNAs positive cases were of intestinal type (seven cases) according to the Lauren classification, while only one case was of diffuse type. Six cases were unclassifiable according to the Lauren classification, but these tumors showed the characteristic lymphoepithelioma-like carcinoma morphology. Interestingly, the one EBV negative case with the EBV positive lymphoid infiltrate showed this particular pattern as well. Selected examples of EBV positive GC are shown in Figure 1.


Epstein-barr virus in gastro-esophageal adenocarcinomas - single center experiences in the context of current literature.

Genitsch V, Novotny A, Seiler CA, Kröll D, Walch A, Langer R - Front Oncol (2015)

Examples of EBER staining patterns and morphology in gastric carcinomas. (A,B) “Lymphoepithelioma-like” morphology; EBER positive [(A) HE; (B) EBER-ISH], (C,D) “intestinal type” morphology, EBER positive [(C) HE; (D) EBER-ISH]; (E,F) “lymphoepithelioma-like” morphology; EBER negative in the tumor, but positive in accompanying lymphocytic infiltrate [(E) HE; (F) EBER-ISH]; (G,H) “intestinal type” morphology, EBER negative [(G) HE; (H) EBER-ISH] (EBER, EBV-encoded small RNAs; ISH, in situ hybridization).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374449&req=5

Figure 1: Examples of EBER staining patterns and morphology in gastric carcinomas. (A,B) “Lymphoepithelioma-like” morphology; EBER positive [(A) HE; (B) EBER-ISH], (C,D) “intestinal type” morphology, EBER positive [(C) HE; (D) EBER-ISH]; (E,F) “lymphoepithelioma-like” morphology; EBER negative in the tumor, but positive in accompanying lymphocytic infiltrate [(E) HE; (F) EBER-ISH]; (G,H) “intestinal type” morphology, EBER negative [(G) HE; (H) EBER-ISH] (EBER, EBV-encoded small RNAs; ISH, in situ hybridization).
Mentions: EBV-encoded small RNAs positive cases were of intestinal type (seven cases) according to the Lauren classification, while only one case was of diffuse type. Six cases were unclassifiable according to the Lauren classification, but these tumors showed the characteristic lymphoepithelioma-like carcinoma morphology. Interestingly, the one EBV negative case with the EBV positive lymphoid infiltrate showed this particular pattern as well. Selected examples of EBV positive GC are shown in Figure 1.

Bottom Line: No association between EBV and pT, pN, or tumor grading was found, neither was there a correlation with clinical outcome.In conclusion, EBV does not seem to play a role in the carcinogenesis of EAC.Recent reports, however, have identified specific epigenetic and genetic alterations in EBV-associated GC, which might lead to a distinct treatment approach for this specific subtype of GC in the future.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University of Bern , Bern , Switzerland.

ABSTRACT
Epstein-Barr virus (EBV)-associated gastric carcinomas (GC) represent a distinct and well-recognized subtype of gastric cancer with a prevalence of around 10% of all GC. In contrast, EBV has not been reported to play a major role in esophageal adenocarcinomas (EAC) and adenocarcinomas of the gastro-esophageal junction (GEJ). We report our experiences on EBV in collections of gastro-esophageal adenocarcinomas from two surgical centers and discuss the current state of research in this field. Tumor samples from 465 primary resected gastro-esophageal adenocarcinomas (118 EAC, 73 GEJ, and 274 GC) were investigated. Presence of EBV was determined by EBV-encoded small RNAs (EBER) in situ hybridization. Results were correlated with pathologic parameters (UICC pTNM category, Her2 status, tumor grading) and survival. EBER positivity was observed in 14 cases. None of the EAC were positive for EBER. In contrast, we observed EBER positivity in 2/73 adenocarcinomas of the GEJ (2.7%) and 12/274 GC (4.4%). These were of intestinal type (seven cases) or unclassifiable (six cases), while only one case was of diffuse type according to the Lauren classification. No association between EBV and pT, pN, or tumor grading was found, neither was there a correlation with clinical outcome. None of the EBER positive cases were Her2 positive. In conclusion, EBV does not seem to play a role in the carcinogenesis of EAC. Moreover, adenocarcinomas of the GEJ show lower rates of EBV positivity compared to GC. Our data only partially correlate with previous reports from the literature. This highlights the need for further research on this distinct entity. Recent reports, however, have identified specific epigenetic and genetic alterations in EBV-associated GC, which might lead to a distinct treatment approach for this specific subtype of GC in the future.

No MeSH data available.


Related in: MedlinePlus