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Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes).

Mendoza-Palmero CA, Blasco-Costa I, Scholz T - Parasit Vectors (2015)

Bottom Line: The position of the Ancylodiscoidinae within a larger clade of dactylogyrids (ancyrocephalines) indicates that this subfamily does not represent a natural group.Members of the three clades do not seem to share obvious morphological synapomorphies nor clear patterns in host-parasite associations, zoogeographical distribution or ecology.Future attempts to propose a new classification of the subfamilies in the Dactylogyridae should include the phylogenetically diverse Neotropical dactylogyrids.

View Article: PubMed Central - PubMed

Affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences and Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budĕjovice, Czech Republic. cmpamtheus@yahoo.es.

ABSTRACT

Background: The phylogenetic relationships of dactylogyrids (Monogenea: Dactylogyridae) parasitising catfishes (Siluriformes) from the Neotropical region were investigated for the first time.

Methods: Partial sequences of the 28S rRNA gene of 40 specimens representing 25 dactylogyrid species were analysed together with sequences from GenBank using Bayesian inference, Maximum likelihood and Parsimony methods. Monophyly of dactylogyrids infecting catfishes and the Ancyrocephalinae was evaluated using the Approximately Unbiased test.

Results: The Ancyrocephalinae is a paraphyletic group of species clustering in three main clades as follows: (i) clade A comprising freshwater dactylogyrids from the Holarctic parasitising perciforms clustering together with species (Ameloblastella, Unibarra and Vancleaveus) parasitising Neotropical catfishes; (ii) clade B including species of Dactylogyrus (Dactylogyrinae) and Pseudodactylogyrus (Pseudodactylogyrinae) along with Ancyrocephalus mogurndae, and marine dactylogyrids with cosmopolitan distribution, parasites of scorpaeniforms and perciforms, along with the freshwater Cichlidogyrus and Scutogyrus (infecting African cichlids [Cichlidae]) and (iii) clade C containing exclusively dactylogyrids of siluriforms, freshwater and marine, with Palaearctic, Ethiopian, Oriental and Neotropical distributions; species of Aphanoblastella and Dactylogyridae gen. sp. 4 from the Neotropical region clustering together with species allocated in the Ancylodiscoidinae, along with species of Cosmetocleithrum, Demidospermus and Dactylogyridae gen. spp.

Conclusions: The position of the Ancylodiscoidinae within a larger clade of dactylogyrids (ancyrocephalines) indicates that this subfamily does not represent a natural group. Instead, species allocated to this clade (dactylogyrids of siluriforms along with species of the Ancylodiscoidinae) should be considered as a separate subfamily within the Dactylogyridae. The erection of this taxon requires the search for morphological diagnostic characters in addition to phylogenetic information. A similar strategy should be considered for a new classification of the paraphyletic Ancyrocephalinae. Members of the three clades do not seem to share obvious morphological synapomorphies nor clear patterns in host-parasite associations, zoogeographical distribution or ecology. Clade A should be considered as the Ancyrocephalinae sensu stricto since it includes the type species Ancyrocephalus paradoxus Creplin, 1839. A new subfamily should be proposed to accommodate species currently allocated to Ancyrocephalinae clustering within clade B. Future attempts to propose a new classification of the subfamilies in the Dactylogyridae should include the phylogenetically diverse Neotropical dactylogyrids.

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Molecular phylogeny of the Dactylogyridae estimated by Bayesian inference using partial sequences of the 28S rRNA gene (695 nt long). Species newly sequenced for this study are in bold. Species belonging to Monocotylidae, Tetraonchidae, Pseudomurraytrematidae and Diplectanidae were used as outgroups. GenBank sequence ID precedes species name. Posterior probabilities and Maximum Likelihood followed by Maximum Parsimony bootstrap support values are given above the branches (posterior probabilities <0.90 and bootstrap values <60 not reported).
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Fig1: Molecular phylogeny of the Dactylogyridae estimated by Bayesian inference using partial sequences of the 28S rRNA gene (695 nt long). Species newly sequenced for this study are in bold. Species belonging to Monocotylidae, Tetraonchidae, Pseudomurraytrematidae and Diplectanidae were used as outgroups. GenBank sequence ID precedes species name. Posterior probabilities and Maximum Likelihood followed by Maximum Parsimony bootstrap support values are given above the branches (posterior probabilities <0.90 and bootstrap values <60 not reported).

Mentions: Forty newly generated sequences (900–1,500 bp long) of the partial 28S rDNA fragment of 25 monogenean species, all parasites of catfishes, 23 from the Neotropical region, one from Africa (Schilbetrema sp.) and one from Asia (Thaparocleidus sp.) (see Table 1) were aligned together with 42 published sequences of species belonging to the subfamilies Ancylodiscoidinae, Ancyrocephalinae, Dactylogyrinae and Pseudodactylogyrinae of the Dactylogyridae retrieved from GenBank (see accession numbers in Figure 1 and Table 1). Three sequences of species belonging to the family Monocotylidae of the order Monocotylidea, sister group of the order Dactylogyridea, were used as out groups, together with six sequences of species belonging to families sister to the Dactylogyridae, i.e. Diplectanidae, Pseudomurraytrematidae and Tetraonchidae. Sequences were aligned using default parameters in MUSCLE implemented in GUIDENCE [35]. Two datasets were created: one included a total of 695 nucleotide positions (full alignment) and another with a total of 489 nt positions, in which positions with a score lower that 0.8 were excluded [36].Figure 1


Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes).

Mendoza-Palmero CA, Blasco-Costa I, Scholz T - Parasit Vectors (2015)

Molecular phylogeny of the Dactylogyridae estimated by Bayesian inference using partial sequences of the 28S rRNA gene (695 nt long). Species newly sequenced for this study are in bold. Species belonging to Monocotylidae, Tetraonchidae, Pseudomurraytrematidae and Diplectanidae were used as outgroups. GenBank sequence ID precedes species name. Posterior probabilities and Maximum Likelihood followed by Maximum Parsimony bootstrap support values are given above the branches (posterior probabilities <0.90 and bootstrap values <60 not reported).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4374382&req=5

Fig1: Molecular phylogeny of the Dactylogyridae estimated by Bayesian inference using partial sequences of the 28S rRNA gene (695 nt long). Species newly sequenced for this study are in bold. Species belonging to Monocotylidae, Tetraonchidae, Pseudomurraytrematidae and Diplectanidae were used as outgroups. GenBank sequence ID precedes species name. Posterior probabilities and Maximum Likelihood followed by Maximum Parsimony bootstrap support values are given above the branches (posterior probabilities <0.90 and bootstrap values <60 not reported).
Mentions: Forty newly generated sequences (900–1,500 bp long) of the partial 28S rDNA fragment of 25 monogenean species, all parasites of catfishes, 23 from the Neotropical region, one from Africa (Schilbetrema sp.) and one from Asia (Thaparocleidus sp.) (see Table 1) were aligned together with 42 published sequences of species belonging to the subfamilies Ancylodiscoidinae, Ancyrocephalinae, Dactylogyrinae and Pseudodactylogyrinae of the Dactylogyridae retrieved from GenBank (see accession numbers in Figure 1 and Table 1). Three sequences of species belonging to the family Monocotylidae of the order Monocotylidea, sister group of the order Dactylogyridea, were used as out groups, together with six sequences of species belonging to families sister to the Dactylogyridae, i.e. Diplectanidae, Pseudomurraytrematidae and Tetraonchidae. Sequences were aligned using default parameters in MUSCLE implemented in GUIDENCE [35]. Two datasets were created: one included a total of 695 nucleotide positions (full alignment) and another with a total of 489 nt positions, in which positions with a score lower that 0.8 were excluded [36].Figure 1

Bottom Line: The position of the Ancylodiscoidinae within a larger clade of dactylogyrids (ancyrocephalines) indicates that this subfamily does not represent a natural group.Members of the three clades do not seem to share obvious morphological synapomorphies nor clear patterns in host-parasite associations, zoogeographical distribution or ecology.Future attempts to propose a new classification of the subfamilies in the Dactylogyridae should include the phylogenetically diverse Neotropical dactylogyrids.

View Article: PubMed Central - PubMed

Affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences and Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budĕjovice, Czech Republic. cmpamtheus@yahoo.es.

ABSTRACT

Background: The phylogenetic relationships of dactylogyrids (Monogenea: Dactylogyridae) parasitising catfishes (Siluriformes) from the Neotropical region were investigated for the first time.

Methods: Partial sequences of the 28S rRNA gene of 40 specimens representing 25 dactylogyrid species were analysed together with sequences from GenBank using Bayesian inference, Maximum likelihood and Parsimony methods. Monophyly of dactylogyrids infecting catfishes and the Ancyrocephalinae was evaluated using the Approximately Unbiased test.

Results: The Ancyrocephalinae is a paraphyletic group of species clustering in three main clades as follows: (i) clade A comprising freshwater dactylogyrids from the Holarctic parasitising perciforms clustering together with species (Ameloblastella, Unibarra and Vancleaveus) parasitising Neotropical catfishes; (ii) clade B including species of Dactylogyrus (Dactylogyrinae) and Pseudodactylogyrus (Pseudodactylogyrinae) along with Ancyrocephalus mogurndae, and marine dactylogyrids with cosmopolitan distribution, parasites of scorpaeniforms and perciforms, along with the freshwater Cichlidogyrus and Scutogyrus (infecting African cichlids [Cichlidae]) and (iii) clade C containing exclusively dactylogyrids of siluriforms, freshwater and marine, with Palaearctic, Ethiopian, Oriental and Neotropical distributions; species of Aphanoblastella and Dactylogyridae gen. sp. 4 from the Neotropical region clustering together with species allocated in the Ancylodiscoidinae, along with species of Cosmetocleithrum, Demidospermus and Dactylogyridae gen. spp.

Conclusions: The position of the Ancylodiscoidinae within a larger clade of dactylogyrids (ancyrocephalines) indicates that this subfamily does not represent a natural group. Instead, species allocated to this clade (dactylogyrids of siluriforms along with species of the Ancylodiscoidinae) should be considered as a separate subfamily within the Dactylogyridae. The erection of this taxon requires the search for morphological diagnostic characters in addition to phylogenetic information. A similar strategy should be considered for a new classification of the paraphyletic Ancyrocephalinae. Members of the three clades do not seem to share obvious morphological synapomorphies nor clear patterns in host-parasite associations, zoogeographical distribution or ecology. Clade A should be considered as the Ancyrocephalinae sensu stricto since it includes the type species Ancyrocephalus paradoxus Creplin, 1839. A new subfamily should be proposed to accommodate species currently allocated to Ancyrocephalinae clustering within clade B. Future attempts to propose a new classification of the subfamilies in the Dactylogyridae should include the phylogenetically diverse Neotropical dactylogyrids.

Show MeSH
Related in: MedlinePlus