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Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema.

He ZH, Chen P, Chen Y, He SD, Ye JR, Zhang HL, Cao J - Tob Induc Dis (2015)

Bottom Line: Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured.Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01).There was no significant difference between CS group and CSE group in any of the parameters described above.

View Article: PubMed Central - PubMed

Affiliation: Intensive Care Unit, the Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011 China.

ABSTRACT

Background and objective: Emphysema is the main pathological feature of COPD and also is the focus of the related research. Although several emphysema animal models have been established, exact comparison of findings is seldom. The present study aimed to compare cigarette smoke (CS) exposure-induced emphysema model and intraperitoneal injection of cigarette smoke extract (CSE)-induced emphysema model to evaluate the effectiveness of the two different modeling methods.

Methods: Six-week-old male C57BL/6 J mice were used and randomly divided into two groups: CS exposure and intraperitoneal injection of CSE. Each group was subdivided into two subgroups: control and CS or CSE. Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured.

Results: Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01). There was no significant difference between CS group and CSE group in any of the parameters described above.

Conclusions: Both CS exposure and intraperitoneal injection of CSE could induce emphysema and the effectiveness of the two different modeling methods were equal.

No MeSH data available.


Related in: MedlinePlus

SOD and IL-6 concentrations in serum of C57BL/6 J mice induced by CS exposure (A) and that induced by intraperitoneal injection of CSE (B). SOD, superoxide dismutase; IL-6, interleukin-6; CS, cigarette smoke; CSE, cigarette smoke extract. ★P < 0.01 compared with controls.
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Fig4: SOD and IL-6 concentrations in serum of C57BL/6 J mice induced by CS exposure (A) and that induced by intraperitoneal injection of CSE (B). SOD, superoxide dismutase; IL-6, interleukin-6; CS, cigarette smoke; CSE, cigarette smoke extract. ★P < 0.01 compared with controls.

Mentions: The SOD concentration in the serum of mice induced by CS (215.91 ± 39.44 U/mL, P < 0.01) or CSE (200.93 ± 45.86 U/mL, P < 0.01) were lower than those in their respective controls (272.33 ± 31.26 U/mL and 284.08 ± 36.09 U/mL, respectively). There was no significant difference in SOD concentration (P > 0.05) between CS group and CSE group. The IL-6 concentration in the serum of mice induced by CS (73.54 ± 16.02 pg/mL, P < 0.01) or CSE (70.74 ± 13.43 pg/mL, P < 0.01) were higher than those in their respective controls (8.88 ± 1.56 pg/mL and 10.03 ± 1.87 pg/mL, respectively). There was no significant difference in IL-6 concentration (P > 0.05) between CS group and CSE group (Figure 4).Figure 4


Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema.

He ZH, Chen P, Chen Y, He SD, Ye JR, Zhang HL, Cao J - Tob Induc Dis (2015)

SOD and IL-6 concentrations in serum of C57BL/6 J mice induced by CS exposure (A) and that induced by intraperitoneal injection of CSE (B). SOD, superoxide dismutase; IL-6, interleukin-6; CS, cigarette smoke; CSE, cigarette smoke extract. ★P < 0.01 compared with controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4374374&req=5

Fig4: SOD and IL-6 concentrations in serum of C57BL/6 J mice induced by CS exposure (A) and that induced by intraperitoneal injection of CSE (B). SOD, superoxide dismutase; IL-6, interleukin-6; CS, cigarette smoke; CSE, cigarette smoke extract. ★P < 0.01 compared with controls.
Mentions: The SOD concentration in the serum of mice induced by CS (215.91 ± 39.44 U/mL, P < 0.01) or CSE (200.93 ± 45.86 U/mL, P < 0.01) were lower than those in their respective controls (272.33 ± 31.26 U/mL and 284.08 ± 36.09 U/mL, respectively). There was no significant difference in SOD concentration (P > 0.05) between CS group and CSE group. The IL-6 concentration in the serum of mice induced by CS (73.54 ± 16.02 pg/mL, P < 0.01) or CSE (70.74 ± 13.43 pg/mL, P < 0.01) were higher than those in their respective controls (8.88 ± 1.56 pg/mL and 10.03 ± 1.87 pg/mL, respectively). There was no significant difference in IL-6 concentration (P > 0.05) between CS group and CSE group (Figure 4).Figure 4

Bottom Line: Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured.Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01).There was no significant difference between CS group and CSE group in any of the parameters described above.

View Article: PubMed Central - PubMed

Affiliation: Intensive Care Unit, the Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011 China.

ABSTRACT

Background and objective: Emphysema is the main pathological feature of COPD and also is the focus of the related research. Although several emphysema animal models have been established, exact comparison of findings is seldom. The present study aimed to compare cigarette smoke (CS) exposure-induced emphysema model and intraperitoneal injection of cigarette smoke extract (CSE)-induced emphysema model to evaluate the effectiveness of the two different modeling methods.

Methods: Six-week-old male C57BL/6 J mice were used and randomly divided into two groups: CS exposure and intraperitoneal injection of CSE. Each group was subdivided into two subgroups: control and CS or CSE. Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured.

Results: Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01). There was no significant difference between CS group and CSE group in any of the parameters described above.

Conclusions: Both CS exposure and intraperitoneal injection of CSE could induce emphysema and the effectiveness of the two different modeling methods were equal.

No MeSH data available.


Related in: MedlinePlus